Locked nucleic acids (LNAs) are widely used in RNA therapeutics as LNA-modified antisense oligonucleotides. From the structural point of view, LNA is an RNA derivative in which the ribose ring is constrained by a methylene linkage between the 2′-oxygen and the 4′-carbon. This conformational restriction causes an increased binding affinity to complementary DNA and RNA sequences, but the susceptibility to RNase H should be optimized for individual applications.

Locked nucleic acids (LNAs) are widely used in RNA therapeutics as LNA-modified antisense oligonucleotides . From the structural point of view, LNA is an RNA derivative in which the ribose ring is constrained by a methylene linkage between the 2′-oxygen and the 4′-carbon . This conformational restriction causes an increased binding affinity to complementary DNA and RNA sequences, but the susceptibility to RNase H should be optimized for individual applications.