Autism spectrum disorder (ASD) encompasses a broad range of complex polygenic and multifactorial neurodevelopmental diseases affecting social interaction, communication, interests, and behavior. The major limit to ASD research remains a lack of relevant models which can faithfully re-capitulate key features of the pathology. Recent advances in induced pluripotent stem cells (iPSCs), reprogrammed from somatic cells of patients, have provided a promising cellular tool for disease modelling and development of novel drug treatments. Moreover, iPSCs can be differentiated and organized into 3D organoids, providing a model which mimics the complexity of the brain’s architecture.