1. Introduction

Over the last decades, conventional manufacturing techniques, such as photo-patterning, self-assembly, and soft lithography, have been used for the manufacturing of microfluidic devices and also OoC. In particular, lithography has been the most common technique performed. Nevertheless, this is a highly expensive process with complicated and time-consuming manual procedures performed in a clean-room environment ^{[1][2][3][4][5][6]}[10,15,16,17,18,19]. In this sense, recent advances in microfabrication techniques, cell biology, microfluidics, and 3D printing enabled the rapid manufacturing of OoC along with biomimetic tissue micro-architectures, which can provide the basis for preclinical assays with greater predictive power ^[7][5]. Compared to conventional manufacturing techniques, 3D printing includes the advantages of unlimited design space, freedom of complex geometries, and reduction of waste products ^[8][9][10][20,21,22]. In particular, 3D bioprinting is an innovative and promising biofabrication strategy that has played an important role since it allows the deposition of biomaterial-encapsulated living cells in the manufacturing of complex 3D structures with high precision, high accuracy, and high throughput ^{[11][12][13][14][15][16]}[23,24,25,26,27,28]. Considering those characteristics, bioprinters are expected to establish systems that mimic the microenvironment of the human body in a more appropriate way than the animal models and current 2D cell culture environments, enhancing the accuracy of the results and the clinical usage of OoC ^[13][17][18][25,29,30].

The aforementioned preclinical models used in biomedical investigation are outlined in Figure 1, highlighting the evolution of cell-culture models from simple two-dimensional to complex OoC platforms with 3D bioprinted models.

TFigure 1. Schematic aforementioneddiagram showing preclinical models used in biomedical research.

2. 3D Printing Techniques and Their Applications to Organ-on-a-Chip Platforms

2.1. 3D Printing Techniques

3D prinvestigation are outlineng has become a growing field in Figure 1,different areas and it hias ghlighting the evolution of cell-culture models from simple two-dimensional ained great interest, because of the ability to build complex structures through a layer-by-layer process with different materials in an affordable way [30]. For these reaso complex OoC platforms with 3D bioprintedns, some authors have been using 3D printing techniques to rapidly fabricate the microfluidic models and holders for OoC devices (Table 1).

Figure 1. Schematic diagram showing preclinical models used in biomedical research.

2. 3D Printing Techniques and Their Applications to Organ-on-a-Chip Platforms

2.1. 3D Printing Techniques

3D printing has become a growing field in different areas and it has gained great interest, because of the ability to build complex structures through a layer-by-layer process with different materials in an affordable way ^[18]. For these reasons, some authors have been using 3D printing techniques to rapidly fabricate the microfluidic models and holders for OoC devices (Table 1).

Table 1.

3D (Bio)Printing Technology	Schematic Representation	Ref.
Nervous System-on-a-Chip	Micro-extrusion 3D printing strategies	Reprinted with permission from ref. [26]. Copyright 2001 Royal Society of Chemistry.	Reprinted with permission from ref. [40]. Copyright 2001 Royal Society of Chemistry.	Schwann cells, superior cervical ganglia and hippocampal neurons and epithelial cells	-
Embedded extrusion bioprinting	Reprinted from ref. [42].	Reprinted from ref. [61].	[42]	[61	[26]	[40]
]	Central nervous system-on-a-chip	Magnetic bioprinting
Embedded extrusion bioprinting			Spinal cord cells	[43]	[62]Neural spheroids	[27]	[41]
Multi-tissue OoC with liver, heart and lung organoids	Microextrusion bioprinting
Embedded extrusion bioprinting	Reprinted with permission from ref. [44]. Copyright 2019 Elsevier.	Reprinted with permission from ref. [	Reprinted from ref. [28].	Reprinted from ref. [42].	Hepatocyte; stellate; Kupffer iPS; lung fibroblasts, epithelial, and endothelial cells.	Spherical organoids with HA-gelatin hydrogel (liver) and fibrin-gelatin bioink (cardiac).	[28]	[42]
3D vascularized tissue-on-a-chip	Microextrusion bioprinting	Reprinted from ref. [29].	Reprinted from ref. [43].	hMSCs; hNDFs; HUVECs	Vascular ink (pluronic and thrombin) and cell-laden ink (gelatin–fibrin)	[29]	[43]
Liver-on-a-chip	Direct write bioprinter			HepG2/C3A cells	Hepatic spheroids and GelMA	[30]	[44]
60	]. Copyright 2019 Elsevier.	[44]	[60]
DLW and DLIP	Reprinted from ref. [45].	Reprinted from ref. [63].	[45]	[63]
LIFT printing	Reprinted with permission from ref. [46]. Copyright 2019 Royal Society of Chemistry.	Reprinted with permission from ref. [64]. Copyright 2019 Royal Society of Chemistry.	[46]	[64]	Liver-on-a-chip	Microextrusion bioprinting	Reprinted from ref. [1].	Reprinted from ref. [10].	HepG2; HUVECs.	Gelatin and liver dECM bioinks (collagen type 1)	[1]	[	[31]	[45]
SLA and Bioprinting (BioScaffolder)		[47]	[65]	10	]
Liver-on-a-chip	Microextrusion bioprinting			HepaRG and HUVECs	Gelatin and liver dECM bioinks (collagen type 1)	Liver Fibrosis-on-a-Chip	Microextrusion bioprinting	Reprinted with permission from ref.		[32]. Copyright 2020 American Chemical Society.	Reprinted with permission from ref. [46]. Copyright 2020 American Chemical Society.	HepaRG, HUVECs and hepatic stellate cells	Gelatin and liver dECM bioinks (collagen type 1)	[32]	[46]
Convoluted 3D renal proximal tubules-on-a-chip	Extrusion custom-designed, multi-material 3D bioprinter	Reprinted from ref. [33].	Reprinted from ref. [47].	PTECs-TERT1	Two-part silicone elastomer; Pluronic and thrombin.	[33]	[47]
Vessel-like structures-on-a-chip	Coaxial nozzle-assisted extrusion-based bioprinting	Reprinted with permission from ref. [34]. Copyright 2017 American Chemical Society	Reprinted with permission from ref. [48]. Copyright 2017 American Chemical Society	L929 fibroblasts; endothelial cells and smooth muscle cells	Cell-laden alginate filaments	[34]	[48]
Vessel-on-a-chip	-			HAECs; HASMC and NIH/3 T3 fibroblast cell lines	GelMA	[35]	[49]
Heart-on-a-Chip	Direct write bioprinter with a customized coaxial nozzle	Reprinted with permission from ref.		[36]. Copyright 2016 Elsevier	Reprinted with permission from ref. [50]. Copyright 2016 Elsevier	HUVECs	Alginate-GelMA	[36]	[50]
Myocardium-on-a-chip	Extrusion-based 3D bioprinting	Reprinted with permission from ref. [37]. Copyright 2020 John Wiley and Sons.	Reprinted with permission from ref. [51]. Copyright 2020 John Wiley and Sons.	hiPSC-CSs	Non-mulberry silk-based ink GelMA and PEGDMA	[37]	[51]
Gut-on-a-chip	Dual cell-printing system supplemented with a core-shell nozzle	Reprinted with permission from ref. [38]. Copyright 2018 American Chemical Society.	Reprinted with permission from ref. [52]. Copyright 2018 American Chemical Society.	Caco-2 cells and HUVECs	Cell-laden collagen bioinks	[38]	[52]
Thrombosis-on-a-chip	Embedded extrusion bioprinting	Reprinted with permission from ref. [39]. Copyright 2016 Royal Society of Chemistry.	Reprinted with permission from ref. [53]. Copyright 2016 Royal Society of Chemistry.	HUVECs	GelMA	[39]	[53]
Tumor array-on-a-chip	On-demand array printing			MDA-MB-231 breast tumor cells showed	GelMA	[40]	[54]
Placenta-on-a-chip	Extrusion-based 3D bioprinting	Reprinted with permission from ref. [41]. Copyright 2016 American Chemical Society.	Reprinted with permission from ref. [55]. Copyright 2016 American Chemical Society.	Human placental cell line and hMSCs	GelMA	[41]	[55]

Device	Printing Method	Application		Main Observations	Ref.
Vessel-on-a-chip	-	Produce molds with diverse forms of channels.	Reprinted with permission from ref. [19]. Copyright 2018 John Wiley and Sons.	Reprinted with permission from ref. [32]. Copyright 2018 John Wiley and Sons.	A simple and cytocompatible approach was developed for fabricating hydrogel-based user-defined chips, suitable for the growth of organ or vascularized tissue models.	[19]	[32]
Lung cancer-on-a-chip	Inkjet	3D-printed chip holder and elastomeric microfluidic channels and microfluidic connectors for cell culture media routing on the higher part of the glass.	Reprinted with permission from ref.		[20]. Copyright 2020 Elsevier.	Reprinted with permission from ref. [33]. Copyright 2020 Elsevier.	This lung cancer-on-chip system, includes integrated biosensors for real-time monitoring of physiological events, can be used with any organ tissue or monolayer micro-tumor models for on-chip toxicity studies.	[20]	[33]
Metastasis-on-a-Chip	Plaster-based 3D printing	3D-printed inverted chamber/channel structures as molds.	Reprinted with permission from ref. [21]. Copyright 2016 John Wiley and Sons.	Reprinted with permission from ref. [4]. Copyright 2016 John Wiley and Sons.	This system supports some aspects of the phenomena of metastasis, allowing to study the translocation of metastatic tumor cells from the primary tissue site to the downstream tissue site.	[21]	[4]
Vessel-on-a-chip	Extrusion-based 3D printing	3D printing of channel prototypes with carbopol gel			It is presented a highly affordable and practical approach in the manufacture of PDMS devices with closed fluid channels, which have great potential to reconstitute a human endothelium-on-a-chip	[2]	[15]
Kidney-on-a-chip	FDM	3D-printed template for conventional soft lithography fabrication of PDMS-based OoC	Reprinted with permission from ref. [22]. Copyright 2016 Elsevier.	Reprinted with permission from ref. [34]. Copyright 2016 Elsevier.	It is demonstrated the application of a 3D-printed template and a common cutter machine to provide a simple and affordable fabrication of OoC.	[22]	[34]
Multi-Organ-On-a-Chip	Laser SLA with epoxy resin	Produce master models for the chambers and channels of the fluidic device.	Reprinted from ref. [23].	Reprinted from ref. [35].	This technology allows the design and rapid mass production of OoC devices.	[23]	[35]
Lung-on-a-chip	DLP	3D-printed molds to manufacture a chip model with an open well design and with lower and upper layers to mimic the human lung.	Reprinted from ref. [24].	Reprinted from ref. [36].	The fabrication technique allows the chip to be fabricated in less than a day, and the molds can also be utilized for repeated PDMS casting. Therefore, the technique is robust, cost-effective, and simple.	[24]	[36]