Inhibitors of Cyclin-Dependent Kinases: Comparison
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Protein phosphorylation is a necessary mechanism to drive numerous cellular processes such as cell division, migration, differentiation and programmed cell death. This process is regulated by many enzymes, including cyclin-dependent kinases (CDKs) which phosphorylate proteins on their serine and threonine amino acid residues. The 20 members of CDK family known to this day regulate the cell cycle, transcription and splicing.

  • cyclin-dependent kinase inhibitors
  • cancer
  • cell cycle
  • CDKs
  • CDK inhibitors

1. Cyclin-Dependent Kinases (CDKs)

Protein phosphorylation is a necessary mechanism to drive numerous cellular processes such as cell division, migration, differentiation and programmed cell death. This process is regulated by many enzymes, including cyclin-dependent kinases (CDKs) which phosphorylate proteins on their serine and threonine amino acid residues. The 20 members of CDK family known to this day regulate the cell cycle, transcription and splicing [1]. A number of kinase inhibitors are emerging every day as potential small molecule drugs, with some of them already being approved by the United States Food and Drug Administration (FDA). Moreover, these already approved kinase targeting drugs now account for more than a quarter of all available drugs [2]. In relation to CDK inhibitors, drugs such as Palbociclib

1

, Ribociclib

2

and Abemaciclib

3

, have been approved for ER+/HER2- advanced breast cancer treatment [3]. Until recently, the focus of the research was aimed at the highly conserved ATP binding sites of each CDK kinase. Hence, the development of CDK inhibitors has been extremely challenging due to the difficulty of obtaining sufficient selectivity with typical ATP-mimetic compounds. The greatest number of reported compounds has been identified to target the ATP binding pocket. Most recent studies suggest that inhibitors targeting hydrophobic pockets outside the ATP binding site may provide an opportunity for rational target selectivity [4].

Figure 1

illustrates the typical protein structure of the CDK enzyme. The diagram depicts the structural features of a typical kinase domain. Specifically highlighted are the binding pockets of different types of inhibitors, as well as the activation loop.

Ijms 22 02806 g001 550

Figure 1.

Schematic representation of different types of binding pockets. The protein kinase is shown in blue, with the Asp-Phe-Gly (DFG) motif in green. Red color denotes the aspartate amino acid residue of the DFG motif. The particular regions where different types of inhibitors bind are described below, the allosteric pocket is only a visualization and its place can be anywhere outside the ATP binding site.

2. Cyclin-Dependent Kinase (CDK) Inhibitors in Drug Development

CDK family is known to regulating the cell cycle, transcription and splicing. Deregulation of any of the stages of the cell cycle or transcription leads to apoptosis but, if uncorrected, it can result in a series of diseases such as cancer or neurodegenerative diseases [1,5,6,7].

CDK family is known to regulating the cell cycle, transcription and splicing. Deregulation of any of the stages of the cell cycle or transcription leads to apoptosis but, if uncorrected, it can result in a series of diseases such as cancer or neurodegenerative diseases [1][5][6][7].

Within the last 20 years important advances have been achieved in the development of effective strategies to inhibit CDK kinases. Access of the substrate to the active site of CDK kinase is regulated by the activation loop (A-loop) which is very flexible. The A-loop contains between 20–30 amino acids marked by the conserved Asp-Phe-Gly (DFG) tripeptide motif at the proximal end. Phosphorylation of the activation loop activates the kinase. In this state, the DFG sequence fits snugly into a hydrophobic back pocket adjacent to the ATP binding site. Conversely, in the inactive state the DFG motif swings outwards by partially blocking both the ATP and substrate binding pockets [8].

To date, six types of small molecule kinase inhibitors have been defined by the pharmaceutical industry based on their biochemical mechanisms of action (

Figure 2

). Type I inhibitors interact directly with the ATP binding site and react with the active form of the kinase which is in the DFG-in state and with a phosphorylated activation loop (activation segment). These inhibitors mimic the hydrogen bonds created between the adenine ring of the ATP and the hinge region of the enzyme. Type II inhibitors interact with a DFG-out catalytically inactive conformation of the enzyme and, like type I inhibitors, explore the hinge region and the adenine binding pocket. Type III inhibitors are non-competitive with ATP as they bind to the hydrophobic pocket next to the ATP-binding site, while type IV inhibitors bind away from the ATP binding pocket. Both, type III and IV inhibitors are allosteric in nature [8]. Type V inhibitors interact with two separate regions of the protein kinase domain. This group of inhibitors has been classified as bi-substrate inhibitors. These five classes of inhibitors interact reversibly, while type VI inhibitors form a covalent bond with their target kinase (

Figure 2

) [9].

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Figure 2.

Graphical illustration of different types of kinase inhibitors and their mode of action. Dark red hexagon represents an inhibitor. The protein kinase is shown in blue, the DFG motif in green, the aspartate amino acid residue of the DFG motif in red. In 2015 Wu demonstrated that co-crystal structure of 3-phosphoinositide-dependent protein kinase 1 (PDPK1, PDK1) with ATP showed that type I inhibitors interact with the active conformation of the enzyme where the aspartate residue of the DFG motif points into the ATP binding pocket, while type II inhibitors stabilize the inactive conformation of the enzyme where the aspartate residue faces outward of the binding site (PDB entry: 4RRV). Type III inhibitors interact with the allosteric site within the ATP binding pocket. Type IV inhibitors interact with the allosteric site. However, the allosteric pocket is only a visualization and its place can be anywhere outside the ATP binding site. Type V inhibitors interact with both the allosteric and ATP binding pockets. Type VI inhibitors form covalent bonds with either the ATP binding pocket or the allosteric pocket.

3. Type I Inhibitors

Many heterocyclic compounds can mimic the hydrogen binding motif of adenine, therefore many type I inhibitors have been discovered. As mentioned above, these compounds interact with the ATP-site of the kinase in its active (DFG-in) conformation and with phosphorylated polypeptide region (activation segment) which lies outside the active site pocket. First generation of structurally diverse ATP competitive small molecule type I CDK inhibitors, produced in the late 1990s and early 2000s, have entered clinical trials to treat numerous solid tumors and hematopoietic malignances. Among the list of compounds that have been synthesized as CDK inhibitors, Flavopiridol (Alvocidib)

4

(

Figure 3

), a flavonoid derived from an indigenous plant from India, is active against CDK1, CDK2, CDK4, CDK6, and CDK9 with IC

50

values in the 20–100 nM range (

Table 1) [10,11,12,13]. Flavopiridol can inhibit cell cycle progression in G1 as well as G2 phase due to inhibition of CDK2/4 and CDK1 activity, respectively. Early clinical trials proved ineffective because of unsatisfactory efficacy and high toxicity [14,15]. However, later studies confirmed its clinical efficacy in hematological malignancies, and it was granted orphan drug designation for the treatment of patients with acute myeloid leukemia (

) [10][11][12][13]. Flavopiridol can inhibit cell cycle progression in G1 as well as G2 phase due to inhibition of CDK2/4 and CDK1 activity, respectively. Early clinical trials proved ineffective because of unsatisfactory efficacy and high toxicity [14][15]. However, later studies confirmed its clinical efficacy in hematological malignancies, and it was granted orphan drug designation for the treatment of patients with acute myeloid leukemia (

Table 2

) [16].

Ijms 22 02806 g003 550
Figure 3.

Chemical structures of some of the most studied type I cyclin-dependent kinase (CDK) inhibitors.

Roscovitine (Seliciclib)

5

(

Figure 3

), one of the best known CDK inhibitors, is active against CDK2, CDK5, CDK7 and CDK9 (

Table 1

). This compound is, by far, the most effective inhibitor of CDK5/p25 (IC

50

= 160 nM [17]), as shown by numerous studies using this compound as a potential drug against cancer, neurodegenerative or viral diseases, inflammation, polycystic kidney disease (PKD) and glomerulonephritis (

Table 2) [18,19,20,21,22]. However, despite many successful preclinical studies, results from several clinical trials are not that promising [23].

) [18][19][20][21][22]. However, despite many successful preclinical studies, results from several clinical trials are not that promising [23].

Another compound is Dinaciclib

6

(

Figure 3

), which proved to be a very effective small molecule inhibitor against CDK5 (IC

50

= 1 nM [24]) (

Table 1

). Preclinical studies have shown that Dinaciclib is effective against solid tumors and chronic lymphocytic leukemia (CLL), without adversely affecting T-lymphocytes and their numbers (

Table 2

) [25].

Moreover, Milciclib

7

(

Figure 3

), an orally bioavailable inhibitor of cyclin-dependent kinases (CDKs) and several other protein kinases responsible for controlling cell growth and replication, has recently obtained the orphan drug designation for thymic carcinoma. It is currently under investigation as a potential drug target for treatment of glioma and hepatocellular carcinoma (HCC) (

Table 2) [26,27]. It inhibits CDK2 with IC

) [26][27]. It inhibits CDK2 with IC

50

of 45 nM and exhibits submicromolar activity against other CDKs including CDK1, CDK4 and CDK5 resulting in a block in the G1 (gap) phase of the cell cycle (

Table 1

) [28]. Furthermore, Milciclib was found to reduce levels of microRNAs, miR-221 and miR-222, which promote the formation of blood supply (angiogenesis) in cancer tumors [29].

And finally, Palbociclib

1

and Ribociclib

2

(

Figure 3

), novel CDK4/6 inhibitors, were approved as effective drugs against HR+/HER2- metastatic breast cancer (

Table 2) [30,31]. They selectively inhibit CDK4/6 (

) [30][31]. They selectively inhibit CDK4/6 (

Table 1

), thereby inhibiting retinoblastoma (Rb) protein phosphorylation early in the G1 phase leading to cell cycle arrest, causing defects in DNA replication and efficiently suppress cancer cell proliferation. Most recent data show that both drugs demonstrate a synergistic effect when combined with other drugs, for example Palbociclib and aromatase inhibitor Letrozole [32], Ribociclib and either anaplastic lymphoma kinase (ALK) inhibitor or the mitogen-activated protein kinase kinase (MAP2K, MEK) inhibitor Trametinib [33]. Moreover, utilizing this approach leads to a significant reduction in the development of resistance during prolonged treatment courses [31].

In addition, Tamoxifen

8

has been found to be effective against breast cancer. It reduces CDK5 activity by interacting with p25 and p35, thus preventing CDK5 activation. Tamoxifen can also lower Tau protein phosphorylation, which may suggest that tamoxifen could be used against Alzheimer’s disease [34].

Yet another inhibitor, 5,6-dichlorobenzimidazone-1-β-D-ribofuranoside (DRB)

9

(

Figure 3

) possesses high selectivity against CDK9, with nearly 25-fold difference in potency over CDK2 and CDK7 (

Table 1

) [35]. In HeLa cells, DRB (75 μM) inhibited 60-75% of nuclear heterogeneous RNA (hnRNA) synthesis. DRB inhibited a HeLa protein kinase which phosphorylated an RNA polymerase II-derived peptide [36]. DRB can also inhibit HIV transcription (IC

50

= ~4 μM) by targeting elongation enhanced by the HIV-encoded transactivator Tat (

Table 2

) [37].

Table 1.

Kinase inhibitory activities of type I CDK inhibitors.

Inhibitor Kinase IC50 [nM]

Type I CDK inhibitors at different phases of clinical and pre-clinical studies. Trial information obtained from

ClinicalTrials.gov

as of January 2021.

Inhibitor Main Targets Condition or Disease Phase Status Identifier
CDK1/B CDK2/A CDK2/E CDK4/D CDK5/p25 CDK6/D CDK7/H CDK8/C CDK9/T1
Flavopiridol 4 [38,39] [38][39] 30 100 100 20–40 - 60 110 - 20
Flavopiridol 4 CDK1, CDK2, CDK4, CDK6, CDK9 Acute Myeloid Leukemia (AML) on the market “orphan drug” -
Roscovitine 5 [40] Roscovitine 650 700 700 >100,000 160 >100,000 460 5>100,000 600
CDK2, CDK7, CDK9 Pituitary Cushing Disease II active NCT02160730

NCT03774446		 RO-3306 17 [41] 35 - 340
Cystic Fibrosis II>2000 - - - - -
terminated NCT02649751 Dinaciclib 6 [42] 3 1 1 100 1 - - -
Advanced Solid Tumors4
I terminated NCT00999401 Milciclib 7 [28] 398 45 363 160 265 - 150 - -
Lung Cancer Palbociclib 1 [43] >10,000 >10,000 >10,000 11 >10,000 15 - - -
Ribociclib 2 [44] 113,000 76,000 76,000 10 43,900 39 - - -
II terminated NCT00372073
RO-3306 17 [41] CDK1 Acute Myeloid Leukemia (AML) pre-clinical - -
Dinaciclib 6 CDK1, CDK2, CDK5, CDK9 Chronic Lymphocytic Leukemia (CLL) on the market “orphan drug” - Abemaciclib 3 [45] 1627 - 504 2 355 10 3910 - Breast and Lung Cancers57
II terminated NCT00732810 BS-181 18 [46] 8100 730 880 33,000 3000 47,000 21 - 4200
Milciclib 7 CDK1, CDK2, CDK4, CDK7 Hepatocellular Carcinoma (HCC) II active NCT03109886 DRB 9 [47] 17,000 - >10,000
Thymic Carcinoma II terminated NCT01301391

NCT01011439		>10,000 Meriolin 3 12 [48] 170 11 - >100,000
Palbociclib 1 CDK4, CDK6 HR+/HER2- Breast Cancer on the market170 >100,000 >100,000 - 6
used in combination with Letrozole - Variolin B 10 [49] 60 80 - >10,000 90 >10,000 >1000 - 26
III active, to be used with other drugs like Fulvestrant NCT02692755 Meridianin E 11 [50] 180 800 1800 3000 150 - - - 18
Head and Neck, Brain, Colon, and other Solid Cancers II active, to be used alone and in combination with different drugs NCT02255461

NCT03446157

NCT02896335

NCT03965845		 Nortopsentins 13 [51] 310–900 - - -
Ribociclib 2 CDK4, CDK6 HR+/HER2- Breast Cancer on the market- - - - -
used in combination with Letrozole - AZD5438 15 [52] 16 5
- - >10,000 >10,000 340 45 6 449
III14 21 821 - active, to be used with other drugs like Fulvestrant20
NCT02422615

NCT03439046

NCT03294694		 Roniciclib 19 [53] 7 - 9 11 - - 25
Prostate, and other Solid Cancers II- active, to be used alone and in combination with different drugs NCT02555189

NCT01543698

NCT02934568		 SNS-032 16 [54] 480 38 48 925 340

(CDK5/p35)		 - 62 - 4

Table 2.

Abemaciclib
3
CDK4, CDK6
HR+/HER2- Breast Cancer
on the market
used in combination with Fulvestrant
-
III
active, to be used with other drugs like Letrozole NCT02763566
Lung, Brain, Colon, and other Solid Cancers II or III active, to be used alone and in combination with different drugs NCT04545710

NCT02152631

NCT03220646

NCT04616183

NCT03310879
BS-181 18 [46] CDK7 Breast, Lung, Prostate and Colorectal Cancers pre-clinical - -
DRB 9 [55] CDK7, CDK8, CDK9 HIV Transcription pre-clinical - -
Meriolin 3 12 [48] CDK1, CDK2, CDK5, CDK9 Neuroblastoma, Glioma, Myeloma, Colon Cancer pre-clinical - -
Variolin B 10 [56] CDK1, CDK2, CDK5, CDK9 Murine Leukemia pre-clinical - -
Meridianin E 11 [57] CDK1, CDK5, CDK9 Larynx Carcinoma, Myeloid Leukemia pre-clinical - -
Nortopsentins 13 [58] CDK1 Malignant Pleural Mesothelioma (MPM) pre-clinical - -
AZD5438 15 CDK1, CDK2, CDK5, CDK6, CDK9 Advanced Solid Malignancies I terminated NCT00088790
Roniciclib 19 CDK1, CDK2, CDK4, CDK7, CDK9 Lung and Advanced Solid Cancers II terminated NCT02161419

NCT01573338

NCT02656849
SNS-032 16 CDK2, CDK7, CDK9 Chronic Lymphocytic Leukemia and other Solid Cancers I terminated NCT00446342

NCT00292864

Novel alkaloids, acting as CDK inhibitors, were also found in some marine organisms. Variolins, 7-azaindole based alkaloids isolated from the antarctic sponge Kirkpatrickia variolosa [59,60], showed in vitro activity against a murine (P388) leukemia cell line with submicromolar potencies by preventing cell proliferation, and inducing apoptosis (

Novel alkaloids, acting as CDK inhibitors, were also found in some marine organisms. Variolins, 7-azaindole based alkaloids isolated from the antarctic sponge Kirkpatrickia variolosa [59][60], showed in vitro activity against a murine (P388) leukemia cell line with submicromolar potencies by preventing cell proliferation, and inducing apoptosis (

Table 2) [56,59]. Variolin B

) [56][59]. Variolin B

10

(

Figure 3

), in particular, was found to inhibit CDK1 and CDK2 kinases, in the micromolar concentration range (

Table 1

) [61]. Meridianins A-G, a family of 3-(2-aminopyrimidine)indoles, which originate from the ascidian Aplidium meridianum, were demonstrated to inhibit several protein kinases, especially Meridianin E

11

(

Figure 3

), which can selectively inhibit CDK1 and CDK5 in the low micromolar range (

Table 1

) [62]. Based on the latter two compounds, Meriolins

12

, a new class of inhibitors, have been designed. These new derivatives have been reported to strongly inhibit various protein kinases, especially CDK1, CDK2, CDK4 and CDK9 (

Table 1

) [48]. Most recent analysis provides a high potential of Meriolins in the treatment of cancer and noncancer pathologies such as polycystic kidney disease, neurodegenerative diseases, stroke, chronic inflammation, and bipolar disorders (

Table 2

) [48]. Nortopsentins A-C

13

(

Figure 3

), antifungal 1,4-bisindolylimidazole marine alkaloids, having an imidazole as a spacer between the two indole units, isolated from the Caribbean deep sea Spongosorites ruetzleri, displayed in vitro cytotoxicity against P388 leukemia cells (IC

50

4.5–20.7 µM). Analogues in which the imidazole ring of the alkaloid was replaced by other five or six membered heterocycles were able to inhibit the activity of the cyclin-dependent kinase 1 (CDK1) with submicromolar IC

50

values (in particular 3-[(2-indolyl)-5-phenyl]-pyridines, phenyl-thiazolyl-7-azaindoles, indolyl-thiazolyl-4-azaindole and indolyl-thiazolyl-7-azaindole derivatives) (

Table 1

) [51]. Preliminary results indicate, that Nortopsentins, and their analogues, were active against malignant pleural mesothelioma (MPM), a very aggressive human malignancy poorly responsive to currently available therapies (

Table 2

) [58].

Recent development has enabled combinatorial treatment regimens which can demonstrate synergistic anticancer mechanisms. For instance, THZ1

14

(Figure 7) a covalent CDK7 inhibitor, was found to selectively downregulate CDK7-mediated phosphorylation of RNA polymerase II, indicative of transcriptional inhibition. Further investigations revealed that the survival of triple negative breast cancer (TNBC) cells relied heavily on the B-cell lymphoma 2 (BCL-2)/B-cell lymphoma-extra large (BCL-XL) signaling axes in cells. Thus, combining the CDK7 inhibitor THZ1 with the BCL-2/BCL-XL inhibitors (ABT-263/ABT199) offer a preclinical proof to significantly improve the poor prognosis in TNBC [63].

However, the complexity of CDK biology and the undesired toxicity related to the off-target effects of the existing pan-CDK inhibitors, led to decisions by several pharmaceutical companies to discontinue the development of many potential anti-cancer agents, exampled with AZD5438

15

, Roniciclib, SNS-032

16

, RO-3306

17

, BS-181

18

and Roniciclib

19

(

Figure 3

) (

Table 1

and

Table 2) [64,65,66]. Therefore, new classes of more selective CDK inhibitors, with strong potential to deliver a meaningful therapeutic impact, were needed.

) [64][65][66]. Therefore, new classes of more selective CDK inhibitors, with strong potential to deliver a meaningful therapeutic impact, were needed.

One of those compounds is CDK5 inhibitory protein (CIP), a small protein which contribute to nerve cells’ degeneration. CIP specifically blocks the hyperactivated state of CDK5 only when it is linked to p25/p29, while allowing normal activation of CDK5 by p35/p39. The selective inhibition of p25/CDK5 hyperactivation in vivo, through overexpression of CIP, reduced neurodegeneration and improved cognitive function of transgenic mice, without affecting normal neurodevelopment [67]. These findings suggest that CIP could possibly be used to selectively inhibit the p25/CDK5 hyperactivation as a potential therapeutic target to treat certain cancers caused by aberrant CDK5 activation.

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