Eukaryotic Cell | Encyclopedia MDPI

Eukaryotic Cell: Comparison

Please note this is a comparison between Version 2 by Dean Liu and Version 4 by Dean Liu.

Eukaryotic cells, are complex cells that evolved through endosymbiosis when one cell (typically bacterial, forming mitochondria and plastids) is incorporated by a host cell. It might well be that other cellular organelles are also of endosymbiotic nature.

actin
cell
cell biology
cytoskeleton
consciousness
eukaryotes
excitability
membranes
sentience
symbiosis

1. Chimeric Nature of the Eukaryotic Cell

In addition to prokaryotic bacteria and archaea, eukaryotic protozoa are considered to represent unicellular organisms. However, protozoa, as well as all other eukaryotic cells, are complex cells that evolved through endosymbiosis when one cell (typically bacterial, forming mitochondria and plastids) is incorporated by a host cell. It might well be that other cellular organelles are also of endosymbiotic nature. The difficulty is that over geological time, large amounts of DNA can be lost, as in the case of the highly reduced nuclei known as nucleomorphs, in which almost all of the DNA is transferred to the host cell nuclei

^[1][2]

. Similar processes reduced the genome complexity of plastids and mitochondria during their endosymbiont-to-organelle transition for plastids see

^[3]

. Recently, we

^[4][5][6]

discussed the endosymbiotic origin of the eukaryotic nucleus that occurred when a host cell enclosed and endogenized a guest cell of apparent archaeal origin. In this proposal, all the host cell DNA is transferred to the guest cell, which is transformed into the eukaryotic nucleus

^[6]

. In fact, one cannot exclude a putative endosymbiotic origin of several other organelles, such as endoplasmic reticulum, peroxisomes, centrosomes/centrioles and cilia/flagella

^{[7][8][9][10]}

. In cellular evolution, cell–cell merging and endosymbiosis is an ancient and successful strategy, representing a fundamental feature and can also be seen in instances of the secondary and tertiary endosymbiotic events in algae

^{[1][2][3][11][12]}

. In endosymbiosis, tinkering rather than whole-scale re-engineering is obvious

^[13]

when large structures are continuously rearranged and recombined after cellular mergings of the formerly independent unicellular organisms

^[14][15]

23. Structures and Processes Behind Cellular Consciousness—Evolution of Chimeric Consciousness of Eukaryotic Cell

Lynn Margulis was one of the first scientists to seriously discuss the evolutionary origin of cellular consciousness and argued that prokaryotic cells that merged to form chimeric eukaryotic cells had their own prokaryotic-specific sentience

^[8]

. In her view, the original prokaryotic cells had a “protoconsciousess”, and the two merged cells generated a supracellular consciousness. We develop this below from the perspective of the actin- and tubulin-based cytoskeletal elements where the host cell is proposed as a large archaea cell based on the actin cytoskeleton, while the small motile guest cell is based on the tubulin cytoskeleton supported by the centrosome and basal bodies/centrioles that animate eukaryotic flagella

^{[16][4][5][6]}

We recently discussed the biological foundations of cellular consciousness based on how an excitable plasma membrane, densely populated with so-called biological Maxwell demons, such as sensors, receptors, ion channels, transporters, and ATPases, can generate a senomic cellular field

^{[17][18][19][20][21]}

. In the evolutionary origins of the eukaryotic cell (

Box 1

), both the large, actin-based host cell and the smaller guest cell, which relied on the tubulin-based cytoskeleton, were proposed to be ancient archaea

^[4][5][6]

. This may allow the merging of their fields to generate the new stronger and senomic field of an emergent eukaryotic cell. In addition to the excitable plasma membrane and membranes of recycling vesicles, other cellular structures that are capable of contributing to the cellular fields are the large, bundled, vibrating elements of the cytoskeleton (

Box 2

), such as F-actin

^{[22][23][24][25]}

and microtubules

^{[26][27][28][29]}

. Both excitable plasma membrane and cytoskeletal elements have been proposed to generate proto-consciousness of individual eukaryotic cells

^[17][30]

Box 1.

History of Cellular Evolution: From the Prokaryotic Proto-Consciousness to the Eukaryotic Chimeric Consciousness.

Box 2.

Subcellular Structures Supporting Cellular Conscioussness.

Vibrations of excitable polymers contribute to the intracellular electromagnetic fields and can be expected to interact with the field emanating from the excitable plasma membrane. As microtubules act as memristors, as combinations of memory and electromagnetic resistance ^[31], they are well suited to faithfully decode the cellular senomic fields and to act accordingly. Furthermore, microtubules are structurally linked to both the actin filaments as well as the plasma membrane; they are perfectly suited to generate subcellular bioelectric circuits ^[31][32].

34. Structures and Processes behind Cellular Consciousness—Two Types of Nanobrains Generating Consciousness of Eukaryotic Cell

Two ancient cells merging into one resulted in the generation of supracellular chimeric consciousness having four different excitable sources: two plasma membranes, F-actin, and microtubules. The plasma membrane of the host cells, associated with the actin cytoskeleton, produced the senomic fields of contemporary chimeric eukaryotic cells. The guest cell transformed into the eukaryotic nucleus with the centrosome associated with centriole and organizing perinuclear microtubules ^[33]. The plasma membrane and the nuclear envelope/centrosome/microtubules complex can be viewed as two different cellular nanobrains, the origin, of which can be traced back to the two ancient cells, which merged together, forming the first eukaryotic cell ^[4][5][6]. Vibrations of F-actin and microtubules contribute significantly to the cellular electromagnetic field ^[27][28]. As microtubules act both as intracellular nanowires and memristors, they are perfectly suited for the nanobrain roles of the centrosomes/nuclear envelopes, complementing the principle nanobrain of the eukaryotic cell represented by the excitable plasma membrane (Figure 1, Box 1 and Box 2) inherently linked to the actin cytoskeleton.

Figure 1. Plasma membrane and endosomal recycling vesicles-based nanobrain. Schematic depiction of the senome and the N-space episenome in two contrasting cells of multicellular organisms. (A) In the neurons and neuron-like cells, highly active endocytosis and endocytic vesicle recycling results in hypertrophied senome (lilac circle) and N-space episenome (red circle). Such cells are well-informed about their environment and are active in cell–cell communication via their plasma membrane-based nanobrains. (B) In the example of mature red blood cells, there are only minimal activities of endocytosis and endosomal vesicle recycling. Such cells have shrunk their senomes (lilac circle) and N-space episenomes (red circle) based nanobrains. They are socially isolated, with minimal cell–cell communication and highly reduced cellular sensory apparatus.

References

Sarai, C.; Tanifuji, G.; Nakayama, T.; Kamikawa, R.; Takahashi, K.; Yazaki, E.; Matsuo, E.; Miyashita, H.; Ishida, K.I.; Iwataki, M.; et al. Dinoflagellates with relic endosymbiont nuclei as models for elucidating organellogenesis. Proc. Natl. Acad. Sci. USA 2020, 117, 5364–5375.
Nakayama, T.; Takahashi, K.; Kamikawa, R.; Iwataki, M.; Inagaki, Y.; Tanifuji, G. Putative genome features of relic green alga-derived nuclei in dinoflagellates and future perspectives as model organisms. Commun. Integr. Biol. 2020, 13, 84–88.
Sibbald, S.J.; Archibald, J.M. Genomic insights into plastid evolution. Genome Biol. Evol. 2020, 12, 978–990.
Baluška, F.; Lyons, S. Energide-cell body as smallest unit of eukaryotic life. Ann. Bot. 2018, 122, 741–745.
Baluška, F.; Lyons, S. Symbiotic origin of eukaryotic nucleus—From cell body to Neo-Energide. In Concepts in Cell Biology—History and Evolution; Sahi, V., Baluška, F., Eds.; Plant Cell Monographs 23; Springer International Publishing: Cham, Switzerland, 2018; pp. 39–66.
Baluška, F.; Lyons, S. Archaeal origins of eukaryotic cell and nucleus. Biosystems 2021, 203, 104375.
Margulis, L. Serial endosymbiotic theory (SET) and composite individuality. Transition from bacterial to eukaryotic genomes. Microbiol. Today 2004, 31, 172–174.
Margulis, L. The conscious cell. Ann. N Y Acad. Sci. 2001, 929, 55–70.
De Duve, C. The origin of eukaryotes: A reappraisal. Nat. Rev. Genet. 2007, 8, 395–403.
Zachar, I.; Boza, G. Endosymbiosis before eukaryotes: Mitochondrial establishment in protoeukaryotes. Cell. Mol. Life Sci. 2020, 77, 3503–3523.
Patron, N.J.; Waller, R.F.; Keeling, P.J. A tertiary plastid uses genes from two endosymbionts. J. Mol. Biol. 2006, 357, 1373–1382.
Hehenberger, E.; Gast, R.J.; Keeling, P.J. A kleptoplastidic dinoflagellate and the tipping point between transient and fully integrated plastid endosymbiosis. Proc. Natl. Acad. Sci. USA 2019, 116, 17934–17942.
Jacob, F. Evolution and tinkering. Science 1977, 196, 1161–1166.
Agnati, L.F.; Baluška, F.; Barlow, P.W.; Guidolin, D. Mosaic, self-similarity logic, and biological attraction principles: Three explanatory instruments in biology. Commun. Integr. Biol. 2009, 2, 552–563.
Agnati, L.F.; Barlow, P.W.; Baldelli, E.; Baluška, F. Are maternal mitochondria the selfish entities that are masters of the cells of eukaryotic multicellular organisms? Commun. Integr. Biol. 2009, 2, 194–200.
Baluška, F.; Volkmann, D.; Barlow, P.W. Eukaryotic cells and their Cell Bodies: Cell Theory revisited. Ann. Bot. 2004, 94, 9–32.
Baluška, F.; Reber, A. Sentience and consciousness in single cells: How the first minds emerged in unicellular species. BioEssays 2019, 41, e1800229.
Reber, A.S.; Baluška, F. Cognition is some surprising places. Biochem. Biophys. Res. Commun. 2021, in press.
Baluška, F.; Miller, W.B., Jr. Senomic view of the cell: Senome versus Genome. Commun. Integr. Biol. 2018, 11, 1–9.
Miller, W.B., Jr.; Torday, J.S.; Baluška, F. The N-space Episenome unifies cellular information space-time within cognition-based evolution. Prog. Biophys. Mol. Biol. 2020, 150, 112–139.
Miller, W.B., Jr.; Baluška, F.; Torday, J.S. Cellular senomic measurements in cognition-based evolution. Prog. Biophys. Mol. Biol. 2020, 156, 20–33.
Tuszynski, J.A.; Portet, S.; Dixon, J.M.; Luxford, C.; Cantielo, H.F. Ionic wave propagation along actin filaments. Biophys. J. 2004, 86, 1890–1903.
Iglesias, P.A.; Devreotes, P.N. Biased excitable networks: How cells direct motion in response to gradients. Curr. Opin. Cell Biol. 2012, 24, 245–253.
Pal, D.S.; Li, X.; Banerjee, T.; Niao, Y.; Devreotes, P.N. The excitable signal transduction networks: Movers and shapers of eukaryotic cell migration. Int. J. Dev. Biol. 2019, 63, 407–416.
Beta, C.; Gov, N.S.; Yochelis, A. Why a large-scale mode can be essential for understanding intracellular actin waves. Cells 2020, 9, 1533.
Cifra, M.; Pokorný, J.; Havelka, D.; Kucera, O. Electric field generated by axial longitudinal vibration modes of microtubule. Biosystems 2010, 100, 122–131.
Havelka, D.; Cifra, M.; Kučera, O.; Pokorný, J.; Vrba, J. High-frequency electric field and radiation characteristics of cellular microtubule network. J. Theor. Biol. 2011, 286, 31–40.
Kučera, O.; Havelka, D. Mechano-electrical vibrations of microtubules—Link to subcellular morphology. Biosystems 2012, 109, 346–355.
Kalra, A.P.; Eakins, B.B.; Patel, S.D.; Ciniero, G.; Rezania, V.; Shankar, K.; Tuszynski, J.A. All wired up: An exploration of the electrical properties of microtubules and tubulin. ACS Nano 2020.
Baluška, F.; Reber, A. Senomic and ephaptic principles of cellular consciousness: The biomolecular basis for plant and animal sentience. J. Consc. Stud. 2021, 28, 31–49.
Tuszynski, J.A. The bioelectric circuitry of the cell. In Brain and Human Body Modeling: Computational Human Modeling at EMBC 2018; Makarov, S., Horner, M., Noetscher, G., Eds.; Springer International Publishing: Basel, Switzerland, 2018; pp. 195–208.
Tuszynski, J.A.; Friesen, D.; Freedman, H.; Sbitnev, V.I.; Kim, H.; Santelices, I.; Kalra, A.P.; Patel, S.D.; Shankar, K.; Chua, L.O. Microtubules as sub-cellular memristors. Sci. Rep. 2020, 10, 2108.
Baluška, F.; Volkmann, D.; Barlow, P.W. Nuclear components with microtubule-organizing properties in multicellular eukaryotes: Functional and evolutionary considerations. Int. Rev. Cytol. 1997, 175, 91–135.