1. Introduction

Tuberculosis (TB) is still the world’s leading infectious cause of death, according to the World Health Organization (WHO) ^[1][2]. Its etiological agent,

Mycobacterium tuberculosis

Mtb

Mtb establishes a dynamic equilibrium with the host immune system that lasts for lifetime, with no signs or symptoms of disease ^[3][4]. It is assumed that during latency,

Mtb persists in host tissues mostly in a dormant state. Resuscitation from dormancy, which is orchestrated by a set of cell wall hydrolases^{[5][6][7][8][9]}, is a regular event in the homeostasis of 

Mtb infection that continuously replenishes the bulk of replicating bacilli after their elimination by the host immune response^[10][11]. TB reactivation occurs when the equilibrium between

Mtb

Active TB disease is curable with long-lasting multidrug therapeutic regimens, but the emergence of drug-resistant TB represents a major obstacle to future TB care, with important economic and social consequences^[12]. Ambitious goals for better controlling the global TB epidemic can be met with the development of new drug treatments, improved diagnostics, and most importantly the availability of a new and more effective vaccine^[1][2]. At present, and 100 years after its introduction,

Mycobacterium bovis Bacille Calmette and Guérin (BCG) is the only vaccine available for TB control^[13][14], though its protective activity in preventing TB in adults is variable, incomplete, and overall insufficient ^[15][16][17]. There is an urgent need for a new and more effective vaccine, yet poor understanding of the complex relationship between

Mtb and the human immune system, paired with the lack of immunological correlates of protection, makes this endeavor challenging^[18]. 

2. Structural Features of PE_PGRS33, the PE_PGRS Prototype

Knowing the three-dimensional structure of an antigen provides important insights into the understanding of the molecular nature of host–pathogen interactions and of the key epitopes that may serve as a target for the host antibody response. Although structural data on PE_PGRS proteins are not available, insightful information can be obtained by modeling techniques, learning from homologous proteins. Among PE_PGRS proteins, PE_PGRS33 is one of the best studied for its interaction with the immune system and has been considered a model for PE_PGRSs. As such, its putative role as a vaccine candidate is worth being investigated^[19]. PE_PGRS33 is a large protein of 498 residues, with a modular architecture. A search in the PFAM database only identifies a PE domain at the N-terminal region of the protein, a small domain (residues 1–93) that is distributed nearly exclusively in actinobacteria, with only one exception for

Tulasnella calospora

Tulasnellaceae

Figure 1

Figure 1.

_II sandwich motifs are colored green, purple, blue, and cyan.

2.1. The PE Domain

The PE domain takes its name from the conserved Pro–Glu (PE) amino acids at its N-terminus (residues 7–8) ^[20]

Figure 1). This domain is responsible for PE_PGRS33 translocation via ESX5 and cell wall localization, with a significant role of 30 amino acids on its N-terminus^[21]. A search in the PFAM database shows that the PE family is a member of clan EsxAB (CL0352), which also includes the more common PPE family and the WXG100 family, including the well-known antigen ESAT-6 (6 kDa early secreted antigenic target) and CFP-10 (10 kDa culture filtrate protein) in

Mtb

Staphylococcus aureus

Table 2

Figure 2). The crystal structure of the ESX-5-secreted PE25–PPE41 heterodimer in complex with the ESX-5-encoded cytoplasmic chaperone EspG5 shows that EspG5 binds to a highly conserved hydrophobic chaperone-binding sequence on PPE, named as the hh motif ^[22] (

Figure 2).

By binding PPE, EspG5 protects the aggregation-prone hh motif on PPE proteins and keeps the dimers in a secretion-competent state. Consistently, point mutations of this conserved hh motif affect protein secretion ^[22]. Both in the cases of PE25–PPE41 and PE8–PPE15, the binding of EspG5 chaperone does not cause conformational changes in the heterodimers. The two ternary complexes present highly similar structures. A superposition of their structures using DALI produces root mean square deviations (rmsd), computed on the backbone atoms of PE, PPE, and EspG5 chains, of 1.1 Å, 2.1 Å, and 0.5 Å, respectively. Importantly, EspG5 binds the PE–PPE dimers at a location that does not interfere with the signature ESX secretory motif YxxxD/E at the C-terminal side of PE proteins^[26] (

Figure 2

Table 2. Here, we fill this structural gap by adopting homology modeling. The best template was identified by HHPRED as the PE domain the ESX-5-secreted PE8 (PDB code 5xsf, sequence identity 45.8%) and the homology model built using MODELLER^{[27][28][29][30]}. As a result, the homology model of the PE domain of PE_PGRS33 shows that all hydrophobic/aromatic residues are located on one side of the molecule (

Figure 3). This feature, also observed for the PE domains of PE/PPE complexes, suggests that either the PE domain of PE_PGRS33 forms homodimers or it is prone to interact with another protein to form a heterodimer. It is hitherto not clear whether PE_PGRS proteins require a protein partner^[31][25], as in the case of PE/PPE proteins. Indeed, pe_pgrs genes are expressed as single operons. Also, the PE-unique LipY protein does not require a partner to be secreted^[32]. These findings suggest that PE_PGRSs can be stable on their own, albeit being endowed with prone-to-interact PE domains for their functions.

Figure 3.

Mycobacterium tuberculosis

Mtb) as a template (PDB core 4w4k, sequence identity 37%). Hydrophobic and aromatic residues are drawn in stick representation.

Importantly, the PE domain of PE_PGRS33 is required for the protein translocation through the mycobacterial cell wall^{[33][21][28][29][30]}. Once exerted this role, the PE domain is cleaved from the rest of the molecule, leaving the functional PGRS domain floating on the mycomembrane^[31]. Therefore, it is tempting to surmise that some hydrolases may recognize PE_PGRS33 through its PE domain.

Mtb encodes for a number of PE- and PPE-containing serine α/β hydrolases, which are possible candidates as PE_PGRS33 hydrolases ^[34](

Figure 4

M. marinum

Mtb, is known to cleave the lipase LipY^[32]. More investigations are needed to verify which hydrolase is responsible for PE cleavage of PE_PGRS33 and if more hydrolases cleave specific PE_PGRS proteins.

Figure 4.

Mtb proteins (strain H37Rv), which are predicted to embed a serine hydrolase domain^[34]. The last hydrolase, PE_PGRS35, is the

Mtb

M. marinum PecA^[32].

2.2. PGRS Domain Contains Multiple PG

_II Modules

In PE_PGRS proteins, the PGRS domain can vary in size from tens to almost 1800 amino acid residues. Its main feature is the presence of multiple repeats containing the GGA-GGX motif interspersed with unique sequences^[35]. It has been shown that PGRS domains are available on the mycobacterial surface and can directly interact with host components, as TLR2 receptors ^[36][37][38]. To date, the structure of the PGRS domain remains unknown and should be implemented in experimental data.

The lack of structural data on PGRS domains makes the understanding of the role of these domains a hard task. However, a high sequence identity of the C-terminal part of the PGRS domain of PE_PGRS33 exists with the PG

_II

Hypogastrura harveyi

_II

Figure S1

_II

_II

_II

_II

_II

_II

ψ angle^[39]. Typical of PG

_II

_II) helices found in collagen^[40][41]. In the PG

_II

_II

_II

Figure 5

_II

Figure 5).

Figure 5.

_II

_II

A

_II

B

_II helices.

A comparison with the structure of the PE_PGRS33 PG

_II

_II

Figure 6

_II

Figure 5

Figure 6

_II

Table 3) results in weaker immunostimulatory activity, in terms of reduced TNF-α ^[42][37]. By contrast, hydrophobic residues are mostly located on one side of the PG

_II

Figure 6B)^[43]. The accumulation of hydrophobic residues on one side of the sandwich builds a module with one hydrophilic face and one hydrophobic face, and the flat hydrophobic face is supposed to interact tightly with the highly ordered water molecules found at the surface of an ice crystal^[43]. In this respect, the flatness that characterizes this domain is functional for its tight association with the ice surface^[43]. Interestingly, a PG

_II

Salmonella

_II

_II

_II

Figure 6C).

Figure 6.

_II

A

_II

B

C

Salmonella

_II

_II residues is red for negative, blue for positive, green for hydrophobic, and light blue for polar residues.

In contrast to the antifreeze protein sfAFP, it is likely that in the case of both PE_PGRS33 and the bacteriophage S16 long tail fiber, the flatness of the PG

_II sandwich is useful to allow recognition loops to be closely spaced. In both cases, these loops evolve rapidly, as confirmed by their hypervariable nature, in a similar manner as observed for the three hypervariable complementarity-determining regions (CDRs) of immunoglobulins^[44][45]. As in the case of S16 tail fiber, the PG

_II loops of PE_PGRS33 are likely exposed to the host and, as such, the principal targets of antibodies.

3. PE_PGRS33 as a Promising Target of the Humoral Response

Mycobacterium smegmatis (Ms), in a process mainly governed by its PGRS domain^[46][47][48]. Being localized on the outer surface of the 

Mtb

milieu and in the capacity to interact with the host (

Figure 7A). Consistently, PE_PGRS33 was shown to specifically interact with TLR-2^[49][46], though it remains to be determined whether TLR2 requires heterodimerization with TLR1 or TLR6 to properly bind PE_PGRS33, and the role of coreceptors as CD14 or CD36 as well.

Figure 7.

A

B) immune responses to PE_PGRS33 by the host.

Mtb inside the host^[46] (

Figure 7

Mtb internalization in host cells while dampening macrophage antimicrobial responses^[49]. Interestingly, a polyclonal antiserum raised against native PE_PGRS33 was shown to inhibit

Mtb

Mtb Δpe_pgrs33 strain^[50]. This same Δpe_pgrs33

Mtb strain has impaired capacity to enter macrophages, likely due to the lack of interaction with TLR-2^[49][46]. Mice immunized with recombinant PE_PGRS33 were able to combat recombinant

M. smegmatis overexpressing PE_PGRS33 in vivo^[50]. Hence, PE_PGRS33/TLR2 interaction promotes inflammation and tissue damage while favoring

Mtb

Mtb virulence factor^[44]. This experimental evidence provides functional clues to the hypothesis that PE_PGRS33 may serve as a vaccine antigen candidate against TB^[19][51][52]. Antibodies binding PE_PGRS33 on the 

Mtb

Mtb

Mtb-infected and BCG-vaccinated subjects make antibodies against PE_PGRS33, with the key epitopes located mainly in the PGRS domain^[52]. Most of the experimental evidence gathered so far has relied on the use of denatured recombinant PE_PGRS33^[50] making it difficult to identify the relevant epitopes responsible for the interaction with host receptors and potential targets of a protective humoral response.

_II

Figure 7). The identification of these epitopes and the fine characterization at the atomic level of the interaction between TLR2 and PE_PGRS33 would be very useful to tailor effective immunization strategies. Altogether, these studies highlight the potential use of PE_PGRS33 as a target of a neutralizing humoral response against TB^[50].