A recombinant expression system comprises a biological host and a set of engineered genetic elements that together direct the synthesis of a specified gene product. Its functional architecture integrates a coding sequence with transcriptional and translational control elements—including promoters, ribosome binding sites, untranslated regions, and terminators—that operate within the host's molecular machinery to govern when and to what extent the target polypeptide is produced [1][2]. The expression construct may be maintained episomally on a plasmid or integrated into the host genome, and its regulatory configuration, together with host physiology, determines the efficiency of transcript production, translation, folding, and any post-translational processing the host can perform [3]. The system may further incorporate auxiliary components such as signal peptides for secretion, molecular chaperones for folding support, or genetic modifications that enhance protein maturation [4]. The operational integrity of a recombinant expression system depends on the functional compatibility between the engineered construct and the chosen host, and on the coordinated sequence of molecular events that convert the introduced genetic information into the molecular state of the final product [5].
- expression host
- expression construct
- gene regulation
- protein folding
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References
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- Kangsan Kim; Donghui Choe; Dae-Hee Lee; Byung-Kwan Cho; Engineering Biology to Construct Microbial Chassis for the Production of Difficult-to-Express Proteins. Int. J. Mol. Sci. 2020, 21, 990. [CrossRef]