Enzyme inhibition is the reduction of an enzyme-catalyzed reaction rate through an interaction that restricts substrate binding, catalytic turnover, or the availability of active enzyme. Reversible inhibitors associate with particular states of the catalytic cycle, producing kinetic patterns related to their binding relationships with enzyme and substrate [1]. Covalent inhibitors form a chemical bond with the target protein and can produce persistent inhibition after free inhibitor has been removed [2]. Inhibition can be examined by measuring product formation or by testing competition with a probe that labels an active enzyme. Such competition measurements also reveal inhibition across enzyme populations in a biological sample [3][4]. The chemical reactivity and recognition properties of a covalent inhibitor govern which residues and target proteins it modifies [5].
- enzyme kinetics
- reversible inhibition
- irreversible inhibition
- enzyme–inhibitor complex
Enzyme function and inhibition·Molecular Biology·Biochemistry, Genetics and Molecular Biology·Life Sciences
References
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