Enzyme biosynthesis is the cellular production and maturation of enzyme molecules. Protein-enzyme synthesis begins with translation of an encoding messenger RNA, followed by formation of the molecular features needed for catalysis. For iron–sulfur enzymes, specialized machinery assembles the cluster on a scaffold before transfer to the protein [1][2]. Cofactor insertion and protein maturation are coordinated processes involving multiple accessory components [3]. Enzymes entering the secretory pathway undergo folding, assembly, and modifications in the endoplasmic reticulum, including disulfide-bond formation or glycosylation where appropriate [4]. Organellar enzymes can also undergo proteolytic removal of targeting sequences or other maturation segments after transport [5]. These reactions convert the newly synthesized polypeptide into its functional molecular form and establish the catalytic groups, subunit contacts, and cellular location required for activity [2].
- enzyme production
- translation
- protein maturation
- cofactor assembly
Enzyme Production and Characterization·Biotechnology·Biochemistry, Genetics and Molecular Biology·Life Sciences
References
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