Commercial Applications of Chlorella sp. and Spirulina sp.: Comparison
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Microalgae are a large and polyphyletic group of O2-evolving photosynthetic microorganisms, mostly aquatic, comprising prokaryotic cyanobacteria and eukaryotic members. Estimates of the world microalgal production are around 50.000 t/year, Chlorella sp. and Spirulina sp. accounting for more than 90% of the total microalgal biomass production.  These microorganisms have been widely recognized for their nutritional and therapeutic properties; therefore, a significant growth of their market is expected, especially in the nutraceutical, food, and beverage segments.

  • microalgae
  • cyanobacteria
  • Chlorella sp.
  • Spirulina (Arthrospira) sp.
  • nutritional value
  • aquaculture
  • cosmetics
  • skin care

1. Introduction

The Chlorella sp. market is expected to grow at a Compound Annual Growth Rate (CAGR) of 6.3% from 2021 to 2028, reaching USD 412.3 million by 2028 [4][1]. In terms of value, the Spirulina market is much larger and is expected to reach USD 1.1 billion by 2030, at a CAGR of 9.4% from 2023 to 2030 [5][2]. For both markets, the main growth drivers include: (1) the consumers’ greater tendency toward a protein-rich diet; (2) increasing awareness for health and wellness; (3) the growth of the nutraceutical industry; (4) an increase in vegetarianism; (5) a growing demand for natural food colors and other microalgal sourced products, such as omega-3 fatty acids; and (6) the development of innovative Chlorella food and beverage products, and products that include Spirulina as an ingredient.
Europe held the largest share of the overall Chlorella market in 2021 [4][1], where it is cultivated in closed production systems (fermenters or photobioreactors) [6,7,8,9,10,11][3][4][5][6][7][8]. Light can support the growth of Chlorella sp. in the photobioreactors (autotrophic conditions), while sugar-based growth is done in the dark inside fermenters (heterotrophic nutrition). The heterotrophic mode for the cultivation of Chlorella sp. is expected to grow significantly in the next few years due to higher productivity and a lower risk of contamination, water consumption, and use of space [4][1]. As photosynthetic organisms, microalgae have the ability to convert light energy into chemical energy. However, many species, such as Chlorella sp., Spirulina (Arthrospira) sp., can also assimilate and oxidize organic carbon molecules, extracting energy from them [1][9]. This process can occur both in the absence of light, a condition known as heterotrophy, or in the presence of light, referred to as mixotrophy and photoheterotrophy. Although North America is expected to hold the largest share of the Spirulina market in 2030, the market in Europe is expected to register the fastest growth until 2030 [5][2]. Spirulina is cultivated in raceways, in autotrophic conditions, normally inside greenhouses in Europe and outdoor pounds elsewhere in the world [11,12,13][8][10][11].
Chlorella vulgaris held the largest share of the overall market in 2021, but the Chlorella pyrenoidosa or Chlorella sorokiniana segment is expected to grow significantly in the next few years [4][1]. Presently, Chlorella sp. is a genus from the class Trebouxiophyceae, phylum Chlorophyta, and kingdom Plantae [14][12]. The taxonomy of Chlorella sp. has been evolving for decades and currently represents a group of morphologically similar species of polyphyletic origin rather than a natural genus [15][13]. A total of 14 species are now assigned to the genus Chlorella, including both C. vulgaris and C. sorokiniana. Conversely, C. pyrenoidosa is no longer a valid name and most strains formerly identified as C. pyrenoidosa have been renamed, although C. pyrenoidosa strains in the literature and listed in culture collections can be found; in this case, C. pyrenoidosa refers to strains of uncertain taxonomic status, which have not been examined for reassignment yet [15][13]. In this research, researchers will consider the name C. pyrenoidosa as it appears in the literature or web sources.
Regarding Spirulina sp., a clarification is necessary. Both Spirulina sp. and Arthrospira sp. include cyanobacterial species very similar to each other, but the two genera are taxonomically distinct [16][14]. Many species listed in the past as Spirulina sp. have more recently been included in the genera Arthrospira sp., comprising all those grown commercially and sold as Spirulina [16,17][14][15]. Therefore, the trade name continues as Spirulina with no italics. The most important species of Arthrospira sp. exploited for commercial mass cultivation include Arthrospira maxima, Arthrospira fusiformis, and Arthrospira platensis.

2. Human Food and Nutrition

In 2021, the nutraceutical sector dominated the Chlorella market, due to the distinct properties and benefits of Chlorella as a “healthy food” that contributes to a healthy immune system and body [4][1]. The increasing consciousness regarding health and well-being, as well as the expansion of the nutraceutical industry, highly contribute to maintaining the nutraceutical segment atop the Chlorella global market. Regarding Spirulina, the nutraceutical segment accounted for the largest share of the market, but the food and beverages segment is expected to grow significantly up to 2030, mainly due to the increasing demand for phycocyanin [5][2]. The current dominance of the nutraceutical sector in both Chlorella and Spirulina markets is justified by their high nutritional value. Although their microalgal composition varies with culture age and cultivation conditions [18[16][17][18],19,20], Chlorella sp. and Spirulina (Arthrospira) sp. are characterized by their high protein content, low fat, suitable amino-acid profile, high concentration of vitamins (including B12), omega-3 and omega-6 fatty acids, minerals (potassium, calcium, magnesium, selenium, zinc, and others), and bioactive compounds (Table 1).
Table 1. Nutrient composition of Chlorella and Spirulina [17,21,22,23,24,25,26,27,28].
Nutrient composition of Chlorella and Spirulina [15][19][20][21][22][23][24][25][26].
Nutrient Composition Chlorella Spirulina
Macronutrient (% dry weight)    
  Protein 42–65.5 52–72
  Carbohydrate 8.1–65 9–25
  Lipid/fat 1.6–40 1–8
  Fiber 1.6–6 2–18
  Minerals/Ash 6.3–27.3 3–13
Essential amino acids (mg/g protein)    
  Leucine 40–95 56–84
  Phenylalanine 20–96 29–48
  Lysine 35–82 35–51
  Valine 28–78 29–54
  Isoleucine 1.0–44 1.2–41
  Threonine 40–62 30–62
  Histidine
Table 3). Bioactive compounds from Chlorella sp. and Spirulina (Arthrospira) sp., with their antioxidant and free radical scavenging abilities, are valuable in cosmetics and skincare products for their anti-aging and wrinkle-reducing potential [59,60][57][58]. Carotenoids and peptides offer excellent UV protection in creams and sunscreens, while polysaccharides are ideal for moisturizing purposes, helping to maintain the skin’s water barrier and oil balance.
Table 3.
Bioactive compounds found in
Chlorella
sp. and
Spirulina
(
Arthrospira) sp. that are most relevant for cosmetic applications, and their corresponding activities [57,58].
) sp. that are most relevant for cosmetic applications, and their corresponding activities [55][56].
Bioactive Compound Biological Activity
Carotenoids Scavenges free radicals, fights wrinkles, delays aging, soothes eye skin.

Antioxidant, anti-inflammatory.

Provides blue light and UV protection.

β-Carotene serves as a natural colorant in cosmetics.

Lutein promotes regeneration of normal retinal blood vessels.
Vitamin C Prevents melanin deposits, whitens the skin.

Repairs the skin barrier, capillaries, and photo-aging skin, reduces erythema and telangiectasia, and lightens skin wrinkles.

Stimulates collagen synthesis in the skin.
Vitamin E Antioxidant.

Repairs the skin barrier, treats some skin diseases.
10–35
6.0–28
  Methionine
6.0–58
Polysaccharides
16–28
Antioxidant, antibacterial.

Good film-forming properties, reduces water evaporation on the skin surface and provides a moisturizing effect.		   Tryptophan 1.0–24 10–20
Other amino acids (mg/g protein)    
  Aspartic acid 38–109 54–118
  Serine
Peptides Anti-inflammatory. 13–95 23–68
  Glutamic acid 76–137 70–105


Protects skin , reduces UVB and UVC-effects.
Flavonoids

Phenols		 Antioxidant activity.

Stimulates collagen synthesis in the skin, reduces wrinkle formation.		   Glycine 60–105 39–78
  Alanine 82–159
Microalgal lipids play a crucial role in cosmetics, serving various functions [58,61][56][59]. They are used as moisturizing agents, emollients, surfactants, emulsifiers, texturizers, color and fragrance carriers, preservative carriers, and bioactive ingredients. The types of microalgal lipids commonly used in cosmetics include triacylglycerides, waxes, ceramides, phospholipids, sterols, as well as hydrogenated, esterified, and oxidized lipids. Each of these lipids bring unique properties to cosmetic formulations, making them versatile and valuable ingredients in the industry. With the increasing demand for safe and eco-friendly cosmetics and skin care products, ingredients derived from Chlorella sp. and Spirulina (Arthrospira) sp. are set to take on a significant role in the industry. Their potential to provide efficient and sustainable alternatives makes them an appealing option for both cosmetic manufacturers and consumers in search of innovative and conscientious solutions. In this regard, major companies in the cosmetic industry have been incorporating Chlorella sp. and Spirulina (Arthrospira) sp. into their products, especially creams and serums (Table 4).
Table 4.
Examples of cosmetics marketed by major cosmetic companies.
Manufacturer Product Ingredient Ref.
Estée Lauder Perfectionist Pro Chlorella vulgaris extract [62][60]
Thalgo Activ Refining Blocker C. vulgaris extract [63][61]
51–108
  Cysteine
2.0–35
2.0–6.0
  Tyrosine
13–84
  Spiruline Boost collection (booster concentrate, antipollution gel-cream, detoxifying serum, and booster shot mask) Spirulina platensis extract [64,65,66,67][62][63][64][65]
Institut Esthederm Intensive Spiruline collection (serum and crème) Spirulina maxima extract [68,69][66][67]
Nuxe Merveillance LIFT collection (night cream, firming cream, lift eye cream, and firming-activating serum) C. vulgaris oil [70][68]
Algenist Blue Algae Vitamin C™ Dark Spot Blue vitamin C, phycocyanin extracted from S. platensis extract [71][69]
30–48
  Arginine 47–74 4.0–77
  Ornithine 1.2–1.3 nr
  Proline 27–85 20–41
Fatty acids (FA)    
  Saturated 25–33 1 45–56 1
    63–66 2
  Unsaturated 60–70 1 41–52 1
    33.8–37.1 2
   PUFA 36–65 1 30–42 1
    23.1–24.5 2
   ω-3   0.1–0.22
    Alpha-linolenic acid (essential FA) 14–19.3 1 nr
   ω-6   23.1–24.5 2
    Linoleic acid (essential FA) 11–21 1 16–17 1
Vitamins (mg/100 g)    
  B1 (Thiamine) 1.5–2.4 3.5
  B2 (Riboflavin) 4.8–6.0 3.2
  B3 (Niacin) 23.8 12.1
  B5 (Pantothenic acid) 1.3 0.4–25
  B6 (Pyridoxine) 1.0–1.7 0.78
  B7 (Biotin) 191.6 64
  B9 (Folic acid) 0.61–26.9 0.033
  B12 (Cobalamin) 0.1–125.9 0.012–0.24
  C (Ascorbic acid) 15.6–100.0 nr
  E (Tocopherol) 6.0–2787.0 2.8–75
  A (Retinol) 13.2 nr
  K 0.033 2
1 % of the total fatty acids; 2 % of the total lipids; nr—not referenced.
Currently, Chlorella and Spirulina are mainly sold as powder, as well as tablets, extracts, capsules, or flakes [4,5][1][2]. In these forms of commercialization, both Chlorella and Spirulina are sold without any further processing of the biomass, which is only collected and dried. Although both Chlorella and Spirulina are traded as highly valuated dietary supplements, they have been incorporated in ice creams, snacks, muffins, crackers, bars, cookies, pastry cream, bread, smoothies and other beverages, pasta and noodles, yoghurts, jelly gums, and others [25,29,30][23][27][28]. Additionally, it is noteworthy that some companies have actively engaged in the development of food supplements and nutraceutical ingredients with very high added value. For example, AlgoSource has successfully registered Spirulysat® and Spirugrass® [31,32][29][30]. The first is an extract renowned for its elevated phycocyanin content, supplemented with polysaccharides, amino acids, and more. On the other hand, Spirugrass® is a biorefining byproduct of Spirulina characterized by its abundance of amino acids, iron, vitamin K, and beta-carotenes. Chlorophyll is the predominant pigment in Chlorella sp., accounting for 1–2% of its dry weight (Table 2). Thus, the incorporation of Chlorella sp. into certain processed foods, providing them with functional and nutritional value, imparts an intense green color that may not be suitable for the specific food product. Consequently, some companies have been actively involved in the development of honey/gold and white Chlorella as alternative solutions to meet and enhance the emerging consumption demands [33,34,35,36][31][32][33][34]. On the contrary, Spirulina contains phycocyanin, a highly valued pigment known for its natural blue coloration in food (Table 2). Phycocyanin is a blue pigment synthesized by cyanobacteria, including Arthrospira sp. and Spirulina sp. In the food industry, it is mainly used as a natural coloring agent in confectionery products, frostings, ice cream and frozen desserts, gelatin, dessert coatings and toppings, beverage mixes and powders, custards, yogurts, puddings, cottage cheese, breadcrumbs, and ready-to-eat cereals [5][2]. Additionally, it displays antioxidant activity. The phycocyanin market is projected to reach USD 279.6 million by 2030, growing at a CAGR of 28.1% from 2023 to 2030 [37][35]. This market growth is driven by the increasing adoption of phycocyanin for nutraceuticals and the rising demand for natural blue colorants. Phycocyanin is one of the few natural blue colorants approved in the U.S., Europe, and Asia.
Table 2.
Pigment content of
Chlorella
sp. and
Spirulina
(
Arthrospira
) sp. (mg g
−1 dw) [25,30,38,39,40,41,42,43,44].
dw) [23][28][36][37][38][39][40][41][42].
Pigment Chlorella sp. Spirulina sp.
Chlorophylls 1.16–24.0 3.01–17.0
  Chlorophyll-a 0.25–18.3 2.7–10.8
  Chlorophyll-b 0.07–6.81 0.21–0.42
Pheophytins 9.73–26.93 8.24–13.49
  Pheophytin-a 2.31–5.64 nr
Carotenoids 0.24–8.21 0.23–6.5
  Carotenes nr nr
   β-carotene 0.007–7.18 0.02–2.5
  Xanthophylls nr 2.5–4.7
   Astaxanthin 0.25–6.8 0.095–0.72
   Canthaxanthin 0.67–1.17 0.44–0.65
   Lutein 0.052–13.8 0.12–1.03
   Violaxanthin 0.010–0.037 nr
   Zeaxanthin 0.074–7.00 0.028–2.0
Phycobiliproteins    
  Phycocyanin Absent 95–251
nr—not referenced.
Carotenoids are lipophilic compounds found in Chlorella sp. and Spirulina sp., which have seen an increased growth in market potential through their uses in foods, pharmaceuticals, cosmetics, animal feed, and as dietary supplements [45][43]. For instance, carotenoids (β-Carotene) derived from Chlorella sp. are sold at USD 300–700 per kg in the open market [46][44]. Carotenoids such as lutein have also shown great health-promoting properties such as anti-inflammatory properties, and it has been shown that this bioactive pigment can promote or prevent age-related macular disease which is the leading cause of blindness and vision impairment [47][45].

3. Aquaculture and Aquarists

In 2020, the total aquaculture animal production reached 87.5 million tonnes, worth USD 264.8 billion, and it is expected to reach 106 million tonnes in 2030 [48][46]. Sustainable and innovative aquaculture development remains critical to supply the growing demand for aquatic animal foods. To support this trend, the exploitation of alternative, non-traditional protein and oil sources, such as microalgae, for aquafeeds and feeding is necessary. In fact, the nutritional composition and profile of both Chlorella sp. and Spirulina (Arthrospira) sp., as outlined in the preceding, offer advantages when used as a supplement in fish and other aquatic animals. In aquaculture, microalgae can be introduced in two distinct stages: as food for zooplankton, which then serves as nourishment for fish and their larvae; and incorporated into feed for the nutrition of adult fish and as a substitute for fishmeal or fish oil. When it comes to feeding zooplankton, aquaculture companies typically have their own microalgae production systems. Regarding the incorporation of microalgae in fish feed, feed companies are the main driving force behind change. However, there is not much information available on this topic. The potential of introducing Chlorella sp. and Spirulina (Arthrospira) sp. into fish feed is recognized due to their known benefits. In a similar area, some companies are already producing food for aquarium fish with Chlorella or Spirulina [49,50][47][48]. Microalgae species such as Chlorella sp. are typically used in finfish hatcheries as a food source for rotifers or Artemia sp. [51][49]. This method results in the bioaccumulation of essential nutrients such as fatty acids, amino acids, carotenoids, vitamins, and minerals from the microalgae, thereby boosting the nutritional value of zooplankton. Zooplankton that has been fortified with microalgae has shown potential as an effective enrichment strategy for promoting the growth and survival of fish and shellfish larvae. For instance, the continuous feeding of loach larvae (Misgurnus anguillicaudatus) with live Moina sp. and Daphnia sp., fortified with Chlorella sp., has led to improved growth and survival rates. Similarly, African sharptooth catfish (Clarius gariepinus) exhibited enhanced growth and survival rates when fed a combination of microalgae and zooplankton (including Chlorella sp. and Moina micrura). The survival and growth performance of freshwater carp (Catla catla) significantly improved when fed Cyclops sp. enriched with A. platensis. Fish that were fed M. micrura fortified with Chlorella sp. showed a significant improvement in specific growth rate and survival. Furthermore, the highest growth and survival rates of Betta splendens were observed in fish that were fed copepod fortified with Chlorella sp. Chlorella sp. and Spirulina (Arthrospira) sp. have the potential to replace fish oil and fish meal in diets, enhancing growth and meat quality, stimulating immunity, and improving the pigmentation in various fish species [26,51,52,53,54,55][24][49][50][51][52][53]. They promote growth in a variety of species, including carp, tilapia, Asian seabass, and Nile tilapia. In addition to growth enhancement, these microalgae have been proven to stimulate the immune systems of various fish species [26,51,52,53][24][49][50][51]. More precisely, Chlorella sp. and Spirulina (Arthrospira) sp. stimulate phagocytosis, increase white blood cell count, and enhance the expression of cytokine genes, playing a crucial role in immune response. Spirulina (Arthrospira) sp., rich in carotenoids, enhances pigmentation in fish and neutralizes the toxicity of heavy metals, particularly copper and arsenic [51,52,55][49][50][53]. It also improves reproductive performance by increasing egg production and hatching rates [52][50]. Moreover, in the realm of aquaculture, there exists a widely accepted understanding that the survival rate of fish larvae experiences a remarkable surge when nurtured using live feeds rich in carotenoids, such as rotifers, Artemia sp., and copepods [56][54].

4. Cosmetics and Skin Care

Bioactive compounds and lipids derived from microalgae are increasingly being recognized as potential alternatives to conventional synthetic ingredients in the cosmetic and skin care sectors [57,58][55][56]. As such, extracts from microalgae or specific bioactive compounds can be integrated into the production of a wide range of cosmetic products, including eyeliners, lipsticks, eye shadows, moisturizers, facial cleansers, shampoos, sunscreens, and beauty masks. The bioactive compounds most commonly utilized in cosmetics include carotenoids, polysaccharides, peptides, and vitamins (

Furthermore, it is worth noting that certain companies have been actively involved in the creation of exclusive or even patented ingredients for use in cosmetics. For instance, Algenist created the Blue Algae Vitamin C™, an active L-Ascorbic acid with a blue color, from Spirulina [72][70]. AlgoSource formulated Spiruderm®, a liquid Spirulina extract highly concentrated in phycocyanin, used as an active ingredient for skin moisturizing and re-densifying, and fine lines smoothing [73][71].

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