Basophils and Mast Cells in COVID-19 Pathogenesis: Comparison
Please note this is a comparison between Version 1 by Giuseppe Murdaca and Version 2 by Peter Tang.

Basophils and mast cells are among the principal inducers of Th2 responses and have a crucial role in allergic and anti-parasitic protective immunity. Basophils can function as antigen-presenting cells that bind antigens on their surface and boost humoral immune responses, inducing Th2 cell differentiation. Their depletion results in lower humoral memory activation and greater infection susceptibility. Basophils seem to have an active role upon immune response to SARS-CoV-2. In fact, a coordinate adaptive immune response to SARS-CoV-2 is magnified by basophils. It has been observed that basophil amount is lower during acute disease with respect to the recovery phase and that the grade of this depletion is an important determinant of the antibody response to the virus. Moreover, mast cells, present in a great quantity in the nasal epithelial and lung cells, participate in the first immune response to SARS-CoV-2. Their activation results in a hyperinflammatory syndrome through the release of inflammatory molecules, participating to the “cytokine storm” and, in a longer period, inducing pulmonary fibrosis. 

  • basophils
  • mast cells
  • COVID-19
  • innate immune response
  • adaptive immune response

1. Introduction

The outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which was first reported in Wuhan in December 2019 and is responsible for coronavirus disease 2019 (COVID-19), rapidly spread throughout the world, causing an ongoing, highly contagious, pandemic emergency [1][2][1,2]. The Worldometer coronavirus (www.worldometers.info/coronavirus/ accessed date on 11 October 2021) recorded more than 238,760,310 COVID-19 cases, with 4860 million deaths in 222 countries and territories. Since the beginning, several attempts have been made to defeat the virus, starting from social behavioral strategies, such as social distancing and mask wearing, to pharmacological discoveries (i.e., monoclonal antibodies against S protein) and immunization protocols [3][4][3,4]. Indeed, several vaccine platforms have been studied, with some of them already having received regulatory agencies approval, thus including inactivated, live attenuated, and protein/adjuvant approaches and viral vectors and nucleic acids [5].
SARS-CoV-2 belongs to the beta-coronavirus genus of the family Coronaviridae, consisting of four different genera: alpha-coronavirus, beta-coronavirus, gamma-coronavirus, and delta-coronavirus (ICTV Virus Taxonomy: 2019 Release) [6]. Coronaviruses are characterized by the presence of an envelope and a single-stranded, positive-sense RNA genome 29–30 kb in size. They infect numerous animal species including humans and present high interspecies transfers thus being important zoonotic pathogens. In particular, bats and birds are considered the “natural reservoirs” for human coronavirus zoonotic infections, and all human coronavirus represents the result of a zoonotic transfer (“spillover”) from the animal reservoir, either directly or through an intermediate animal host [7]. Like other human coronaviruses, SARS-COV-2 is an enveloped single-stranded positive RNA (ssRNA) virus. The genome is packed by viral nucleocapsid (N) proteins as a large ribonucleoprotein (RNP) complex and further enclosed by an envelope membrane made of lipids and viral proteins S (surface or spike), M (membrane), and E (envelope) [6], thus forming a sort of “corona”.
The virus enters the target host cells through the S protein, which binds to the angiotensin-converting enzyme 2 (ACE2) receptor on the cell surface through the action of transmembrane protease serine (TMPRSS2) [8][9][8,9], which mediates the cleavage of the S protein itself and initiates the fusion of viral and host membranes. Both ACE2 and TMPRSS2 are expressed in many cell types, with particularly high prevalence in lungs and intestine epithelia and endothelial cells, allowing SARS-CoV-2 to target numerous vital organs. For the same reason, SARS-CoV-2 may thus induce a wide spectrum of symptoms [10]. As an RNA virus, SARS-CoV-2, after entering the host cell, replicates exclusively in the cytoplasm of infected cells, where the viral genome is first unpacked from bound viral N proteins by cellular proteases [6].
When inhaled, SARS-CoV-2 is recognized by pattern recognition receptors (PRRs), thus activating the recruitment of innate and adaptive immune system. Notably, IFNs type I amplify the inflammatory signal, thus promoting inflammatory cytokines, chemokines, and anti-viral enzyme production. At this stage, SARS-CoV-2 locally replicates, and patients are often asymptomatic, but still highly infectious [11]. As viral spread goes on, further cells are infected and viral peptides are presented on MHC I to CD8+ T cells [12][13][12,13], leading to clonal expansion of specific memory cells. Cytotoxic T cells are now activated to clear the virus thanks to perforin and granzyme production [14]. This scenario also includes innate cells co-adjuvate T cells such as macrophages, monocytes, and neutrophils. When the combined action of these players overwhelms the system balance, cytokine storm is induced, often leading to acute respiratory distress syndrome (ARDS) and multi-organ failure [15][16][17][15,16,17].

2. Basophils and COVID-19 Disease

Indeed, according to previous studies on viral infections, basophil depletion might impair the efficacy of IgG-responses to SARS-CoV-2. Considering a comprehensive point of view, basophils could enhance a coordinate adaptive immune response to SARS-CoV-2 that could be suppressed by the hyperinflammatory reaction during the acute phase of COVID-19. Further investigation will be required to understand the mechanisms of basophils in modulating humoral responses to SARS-CoV-2. Interestingly, as the expression of these cells seems to be lower during acute disease, it could be worth determining the grade of this depletion as an important determinant of the antibody response to the virus [18][42]. In the study from Wuhan No. 1 Hospital [19][43], involving 59 male and 68 female Chinese patients, a reduction in basophil count was present in 13.39% of enrolled patients. Moreover, authors noted that the reduction in basophils was present in the first three days of hospitalization and was restored to normal shortly. Fátima Conceição-Silva et al. [20][44] have shown that neutrophils, macrophages, lymphocytes, eosinophils, basophils, and mast cells can produce extracellular traps (ET), even if the modalities are still not completely known. Patients with severe cases of COVID-19 are predisposed to thrombosis in which ETs produced by neutrophils may participate. Contrary to neutrophils, ETs produced by basophils have a protective role against some infections with bactericidal and antifungal activity. A similar activity can also be hypothesized during COVID-19. A multidimensional analysis performed on laboratory parameters and diagnostic test of 178.887 Brazilian individuals, of whom 33.266 resulted in being positive for SARS-CoV 2 [21][45]. A case–control study [22][46] involving 74 COVID-19 patients and 228 non-COVID-19 patients, showed a significant reduction of basophil levels in COVID-19 patients, a result more evident in men older than 25 years of age in the Brazilian study. A similar result was also found in a retrospective study on 120 COVID-19 patients, 100 influenza patients, and 61 healthy controls: basophils were lower both in the COVID-19 group and in the influenza group as compared to controls [23][47]. To further support these findings, researchers conducted an observational, multicentric study [24][48] that compared levels of complete blood count and granulocytes subsets with cytofluorimetric analysis in COVID-19 patients and healthy blood donors as controls. The authors showed a significant decrease in basophil levels in COVID-19 patients when compared to controls. Similar findings came from a retrospective study [25][49] on 548 patients diagnosed with COVID-19 disease performed by Chen et al., in which a difference between on admission and end-hospitalization levels of basophils greater than 0.02 × 109/L (HR, 2.73; 95% CI, 1.5–6.47) represented a risk factor for fatal outcome, thus suggesting that the less is the basophil count on admission the poorer is the outcome of the patient. Analyzing severe cases of COVID-19 disease, in contrast with other low respiratory tract infections and excluding potentially confounding factors such as atopy and use of antihistamine drugs, Laing et al. [26][50] showed a dramatic depletion of plasmacytoid dendritic cells and basophils. A similar lower percentage of basophils in the white blood cell count was found by Qin et al. [27][51] in an observational study involving 452 patients in severe patients compared to non-severe cases (0.1 vs. 0.2%; p = 0.015). Further strength to these results is carried by the study of Sun Y. et al. [28][52] that evaluated the causal association between the different white blood cells and the COVID-19 susceptibility and severity by performing two-sample bidirectional Mendelian randomization analyses from the largest and most recent genome-wide association studies. Considering both severe COVID-19 disease and hospitalization due to COVID-19 disease as outcomes, the authors found an inverse association with low basophil count and low basophil percentage on white blood cell count ((OR = 0.75, CI: 0.60–0.95, p = 0.015), (OR = 0.70, CI: 0.54–0.92, p = 0.011)) and ((OR = 0.83, CI: 0.71–0.97, p = 0.020), (OR = 0.78, CI: 0.65–0.93, p = 0.005), respectively). The authors suggested a possible causal role of the reduced basophil count in increasing the risk of severe COVID-19 disease, potentially due to an insufficient innate immune response to SARS-CoV-2. No associations were found with COVID-19 susceptibility among white blood cells. Contrary to what was observed for basophils, an histopathological study conducted on 6 SARS-CoV-2-confirmed patients compared to 10 H1N1-infected patients and a control group of 10 patients who died for neoplastic or cardiovascular diseases [29][53], showed a striking increase in the number of mast cells in lung of COVID-19 patients. Specifically, mast cells, even in the degranulated form, were more frequently localized in the perivascular spaces between the alveolar sacs and terminal bronchioles and in the alveolar septa, close to the alveolar capillaries. Authors suggested that mast cells may play an important role in triggering the systemic cytokine storm associated with severe COVID-19 because of their production of various mediators, including IL-4 and IL-6, two cytokines involved in COVID-19 disease pathogenesis [30][54]. As previously described, when SARS-CoV-2 infects the host, the host is firstly attacked by innate immune cells, including mast cells. The latter are well expressed by nasal epithelial and lung cells and their activation may be responsible of hyperinflammatory syndrome [31][32][55,56]. Mast cells are ubiquitous in the body, and they are involved in several conditions including viral infections, systemic inflammatory diseases, asthma, neuroinflammatory diseases, traumatic brain injury, stroke, and several stress disorders [20][32][44,56]. Hence, these cells proved to be very heterogeneous, as they may differ in ultrastructure, morphology, mediator content, receptor expression, and responses to various stimuli, thus explaining the different effect they may cause, protective or damaging. In fact, mast cell cytoplasm is enriched of histamine, proteases, heparin, chondroitin sulfate, and proinflammatory and anti-inflammatory cytokines/chemokines filled into granules or de novo produced upon activation, which are released in response to stimuli [33][34][35][57,58,59]. While physiologically these molecules are useful to defeat viral and microbial threats, they can also mediate the inflammatory damage typical of asthma and allergic reactions. Through the same mechanism, SARS-CoV-2 may exploit the same effect of mast cells, inducing an uncontrolled inflammation activation mediated by the release of histamines, proteases, cytokines, chemokines, and arachidonic acid compounds such as prostaglandin D2 and leukotrienes [36][37][38][60,61,62]. Indeed, current reports highlighted that COVID-19 can activate mast cells through TLRs and contribute to pulmonary inflammation and fibrosis [39][40][63,64]. It also emerged, both in basophils and eosinophils, a reduction in the expression of surface CRTH2, a receptor for prostaglandin D2, and a significant increase in programmed cell death ligand 1 (PD-L1) in the severe form of the disease in contrast with mild group. Interestingly, both WHO and SOFA scores correlated with these two findings: positively with PD-L1 and negatively with CRTH2, respectively (Figure 1). The authors suggested that one possible mechanism, shared by other viruses as an escape mechanism, may be the increase of immune checkpoint levels, which usually prevent immune-driven diseases after immune response in tissue in order to avoid the clearance of viral particles during the infection [41][42][65,66]. Moreover, the downregulation of basophil and eosinophil CRTH2, an important activator of T helper 2 polarized response, induces speculation about a possible inhibition of this cell subset by SARS-CoV-2. The authors concluded that this latter mechanism, which is present in allergy and hyper-eosinophilic asthma, can possibly be responsible for the protective effect conferred against SARS-CoV-2 infection and severity by these conditions [43][44][67,68].
Figure 1.
Basophils in COVID-19 pathogenesis.
Interestingly and in line with previous mentioned results, the study performed by Rodriguez et al. [18][42] showed a progressive increase in basophil and eosinophil levels from acute to recovery phase of the disease. They further demonstrated that basophil count rise was correlated to the immunoglobulin G response against SARS-CoV-2. Because of basophils are known to be able to bind antigens on their surface and improve humoral response through the production of IL-4 and/or IL-6, although levels of the latter were found to be inversely associated with specific antibodies, both authors concluded the presence of another mechanism, maybe related to IL-4 production, that may coordinate adaptive and humoral immune response to SARS-CoV-2. In fact, previous studies in viral infection showed similar results, with basophil level associated with humoral response [45][39] and with IL-4 known to enhance B cell activity against infection [46][40]. Moreover, basophil count was inversely correlated with IP-10, an IFN-γ-stimulated chemokine known to present chemo-attractive properties for monocytes, macrophages, T cells, NK cells, and dendritic cells [47][48][69,70]. The authors suggested that the CD68-positive infiltrate revealed by COVID-19 patient postmortem analysis [49][71] might explain the circulating loss of these cell populations, with basophils involved in tissue repair [50][72] and regulation of the coagulation process [51][52][73,74].

3. Mast Cell and COVID-19 Disease

The local inflammatory response in the lung observed in SARS-CoV-2-infected patients is characterized by a complex network of activated inflammatory innate immune cells, fibroblasts, endothelial cells, and bronchial epithelial cells. Bronchial epithelial cells and fibroblasts activated by SARS-CoV-2 cause upregulation of pro-inflammatory cytokines and induction of differentiation of mast cells that release histamine, proteases, cytokines, chemokines, and arachidonic acid compounds, such as prostaglandin D2 and leukotrienes, all of which are involved in the inflammatory network. Histamine is and is released into the vessels after cell stimulation. Histamine, stored endogenously within the secretory granules of mast cells, is involved in the increased expression of chemokine IL-8 and cytokine IL-6, thus favoring the hyperinflammation in the lung. Therefore, in the context of COVID-19 cytokine storm and severe disease, mast cells may act negative factors (Figure 2) as productors of histamine that induce microvascular leakage, proteases, and IL-6 that can degrade matrix, thus favoring intra-alveolar formation of the hyaline membrane and perpetuating inflammation, angiogenic factors and pro-coagulative factors, respectively, that may trigger immune thrombosis [53][54][75,76].
Figure 2.
Role of mast cells in COVID-19.
Interestingly, in this researchtudy, an increased number of IL-4-expressing cells were found in the alveolar septa of severe COVID-19 patients. This finding supports the above-mentioned results of Chen et al. [25][49] and Rodriguez et al. [18][42] regarding a possible mechanism that involves IL-4 in the pathogenesis and recovery phase of the disease. IL-4 is released by Th2 lymphocytes, mast cells, basophils, eosinophils, and innate lymphoid cells, thus representing a possible link between basophils and mast cells in the disease.
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