Soil Biology in the Ecuadorian Amazon

For many decades the Ecuadorian Amazon has been used as source of resources for cities both at national and international level. These facts had important consequences and environmental impacts, affecting from the smallest living organisms of the soil to the indigenous communities and peoples that inhabit the Amazon rainforest, as well as the flora and fauna biodiversity. With the change in land use, the Amazonian territory has been progressively affected and it is gradually decreasing, leaving behind poor soils.  Production conditions result modified by the implementation of large monocultures and livestock systems, a situation that directly affects soil and soil fauna. For this reason, we considered interesting to study, understand and compare the behavior of building organisms in natural and intervened areas, through sampling, inventories and laboratory analysis with the aim of developing and implementing production systems (chakras, agroforestry or silvopastoral systems), which benefit both the small producer and the ecosystem and life that inhabits it. Since there are no easily available compiled papers regarding the "Soil Biology in the Ecuadorian Amazon" in this work we collect information that allows us to offer a framework on the topics of changes in land use, typology of Amazonian soils and its main inhabitats organisms. All these date let to be considered as evidences of the degree of the health/disturbance of the corresponding soils.

All of them constitute the soil biota that is summarized in Table 1, which shows a classification according to the size of the main groups and the main functions that they are known to carry out in the soil.

Group Organism Function
For many decades the Ecuadorian Amazon has been used as source of resources for cities both at national and international level. These facts had important consequences and environmental impacts, affecting from the smallest living organisms of the soil to the indigenous communities and peoples that inhabit the Amazon rainforest, as well as the flora and fauna biodiversity. With the change in land use, the Amazonian territory has been progressively affected and it is gradually decreasing, leaving behind poor soils.
Production conditions result modified by the implementation of large monocultures and livestock systems, a situation that directly affects soil and soil fauna. For this reason, we considered interesting to study, understand and compare the behavior of building organisms in natural and intervened areas, through sampling, inventories and laboratory analysis with the aim of developing and implementing production systems (chakras, agroforestry or silvopastoral systems), which benefit both the small producer and the ecosystem and life that inhabits it. Since there are no easily available compiled papers regarding the "Soil Biology in the Ecuadorian Amazon" in this work we collect information that allows us to offer a framework on the topics of changes in land use, typology of Amazonian soils and its main inhabitats organisms. All these date let to be considered as evidences of the degree of the health/disturbance of the corresponding soils.

Earthworms
They form a network of tunnels, mixing and digging the soil, producing excreta below and above the ground, modifying the water and chemical properties . They transform the physical properties of the soil, benefiting the formation of aggregates, the movement and retention of water, as well as the gas exchange . They act as ecosystem engineers in pore formation, water infiltration and OM humification and mineralization .

Beetles
They participate in litter fractionation and in OM decomposition and mineralization processes . The conservation of the coleoptera family can be a support for possible evaluations of the environmental quality .

Ants
Their mounds are rich in nutrients such as N, P, K, Ca, Mg, and Fe, favoring the proliferation of microflora and micromesofauna . They affect the soil structure, mixing the horizons of the profile and recycling part of the elements that leach from the surface . They modify the physical and chemical properties of the soil , and contribute to the formation of aggregates, water filtration, and aeration .

Termites
They contribute to bioturbation processes , and intervene in the facilitating the transport of excavators, and they can also act as predators .

Mites
Their role is to fragment leaves and dead wood, disperse microbial and fungal spores in the soil. Some species are predators of other

<2 mm Protozoa
They are considered the most important predators of bacteria and fungi. Moreover, they regulate microbial communities, and as pathogenic insects, represent an important biological control .

Fungi
They are involved in processes of decomposition, mineralization, and cycling of nutrients . By forming symbiotic associations, they increase the efficiency of plants to absorb nutrients , increase soil aggregation and participate in the carbon cycle , and allow plants to survive and efficiently absorb phosphorus from the soil . They improve the soil health and plant species growth, provide greater absorption of nutrients, uptake of immobile ions, tolerance to toxic metals, root pathogens, and unfavorable conditions for plants in tropical ecosystems .

Algae
They are photosynthesizing organisms involved in primary production, organic carbon (OC) compounds, and soil structure , and are colonizers. In association with fungi, they form lichens and contribute to soil formation, degrading minerals or rocks by excreting organic acids . From the production of carbohydrates, they form soil aggregates and stop their erosion . Given the variable and morphologically similar nature of the majority, today, they are identified using molecular techniques . [10] [7] [14] [10] [7] [15] [

Contextualization of Ecuadorian Amazon Soil Types, Management, Uses, and Problems
The Ecuadorian Amazon constitutes 45% of the National territory, occupying 115,613 km , extending from the Andean mountain foothills, with the appearance of transitional forests at 1300 m.a.s.l., to the east of the Amazonian plain, forming less than 2% of the Amazon River basin. The average rainfall fluctuates between 2000 and 5000 mm annually, with a temperature of 24 °C during the year, which constitutes a warm damp climate .
The Ecuadorian Amazon has been referred to as "the most important source of fresh water and biodiversity" for its global climate regulatory function as a greenhouse gas sink . It is made up of six provinces: Sucumbíos, Orellana, Napo, Pastaza, Morona Santiago, and Zamora Chinchipe (Figure 1), which have a variety of soil types, from the Andes to soil derived from volcanic ash with specific characteristics . Due to the geological and influence of meteorological factors, poorly developed soils appear with acidic pH, that are susceptible to erosion due to high rainfall, low fertility and nutrient availability, the presence of toxic aluminum for plants, and high moisture retention . According to the American Soil Taxonomy (USDA) system, the soils present in the Ecuadorian Amazon belong to the typological units that are collected in those of order inceptisol, entisol, histosol, and mollisol .

Order Suborder
Alfisol: Mineral soils developed in stable reliefs of erosive tectonic origin, with clays in the surface horizon, and with more than 35% base saturation; because of the natural reserve of nutrients, they are considered good for short-cycle farms and forages.
Entisol: Soils with the scarce formation of horizons, either due to susceptibility to erosion and flooding, or due to the short evolution time; of variable fertility, due to the original material dragged by alluviums or rivers; present agricultural vocation, but the excess of humidity is their main limitation.

Typic Udifluvents
Histosol: Organic soils that are formed when the OM exceeds its mineralization, that are without Indian origins, and are limited in swampy areas that are poorly drained under conditions of continuous saturation that prevents the circulation of oxygen. In this type of soil, sustainable use is limited to forested formations, grasslands, and in some cases, intensive crops. Their ecological importance is because they act as carbon sinks due to the speed of OM contributions they receive.

Aquept Fibrist
Inceptisol: Soils with a lack of edaphological maturity, whose genesis is of rapid formation.
They occupy areas of irregular topography, with both humid and subhumid climates, and have variable chemical and physical properties, from acid to alkaline, sandy to clay. They have-more or less-a base saturation of 60%, and have been used by the agricultural sector for key crops for the economy. They are represented by a forest cover, pastures, and representative crops. Ultisol: Acidic soils, which are products of chemical weathering, from eastern and western Andean hills, present in any humidity regime, with a base saturation of less than 35% providing acidity. These soils are characteristic of humid forests that are part of conservation areas.
Due to the biological richness and endemism of the Ecuadorian Amazon, it is one of the largest ecological reserves, with a high potential to provide ecosystem services to local populations. Being subject to high rates of deforestation and changes in use affects the biodiversity, soil, and water, as basic components of an ecosystem, causing the decrease or loss of possible ecosystem goods and services .
In tropical regions, the change from forests to pastures and agricultural production, on the one hand, leads to losses in terms of fertility, quality, and biodiversity in the soils, and on the other hand, implies the addition of nutrients and amendments to meet the demand for crops; hence, to promote a change in the productive matrix with a sustainable vision, the best strategy is the implementation of agroforestry or silvopastoral systems . This confirms that the Ecuadorian Amazon is not suitable for intensive agricultural activity, but for productive systems similar to the forest . The most common systems are described below.
Silvopastoral systems: Due to the dependence of part of the Amazonian population on livestock activity, it has been necessary to generate production models similar to the forest (silvopastoral systems) . This consists of combining the use of trees, pastures, and animals to increase production, which at the same time brings other benefits, such as wood, biodiversity conservation, carbon fixation, and the protection of water basins and soil . These systems represent a sustainable alternative, which responds satisfactorily to the socio-economic reality of tropical countries, mainly with regard to food demand . In the Ecuadorian Amazon, with colonization, the forests were intervened and used for timber resources, and the establishment of pastures and small orchards . Livestock has turned soils into degraded lands, dependent on mechanization and agrochemicals . Despite this problem, there is an interest in understanding the state of those soils, and implementing strategies or systems compatible with the environment, soil, edaphic life, and livestock farmers . The adoption of silvopastoral systems has increased the biomass in the soil surface, the level of OM in the surface horizon, and thereby the availability and recycling of nutrients, hence improving the soil structural index . On the other hand, the grass influences the system; for example, long-cycle gramalote grass (Axonopus scoparius) does not affect the soil, but forms associations with short-cycle pastures, such as honey grass (Setaria plendida), Amazonian kikuyo (Brachiaria humidicola), dallis (Brachiaria decumbens), or elephant grass (Pennisetum purpureum), in which they can produce important erosion and soil degradation processes .

Crops:
In the Ecuadorian Amazon, many agricultural projects have been promoted with African palm, coffee, cacao, rice, and grassland, from which extensive (monoculture) crops have originated that involve the deforestation of large-scale virgin forest. Projects that expanded the agricultural frontier for state support (credits) . Incentives for planting African palm and palmito, mainly, have caused significant losses in biological biodiversity and vegetation, altering the multifunctionality that ecosystems play .
The management techniques used in monocultures affect the soil community and make them dependent on external inputs , without neglecting the deterioration and degradation of soils that are of little interest to large producers, whose only interest lies in the profitability and economic benefits that they can obtain.
On [7] [37] Agroforestry systems: In Amazonian ecosystems, they fulfil important functions. These systems improve the relationships between the soil, water, and air components of the ecosystem ,39]. When linking crops, such as cocoa or coffee, the damage caused to the soil is minimal. The constant addition of litter and root development that increase the soil's OM produces conditions similar to those of a natural forest . The similarity of conditions of these systems with forests allows the development of processes, such as recycling, fixation, and mineralization of nutrients . They prevent the implantation of monocultures and the impacts of deforestation. At the same time, they generate other economic inputs for producers, such as wood production, firewood, wild fruits; together with environmental services, such as the reduction of greenhouse gases, carbon sequestration, and protection of natural resources (soil, air, water) . They serve as habitats for plant and animal species, from microorganisms, vertebrates, and amphibians of the soil, to large mammals and birds .
In Ecuador and tropical regions in general, the conversion of forests to agricultural and livestock systems is the most important reason for land-use change . These changes lead to the alteration and loss of biodiversity, and mainly to deterioration of the soil .
Forests: The Amazon is characterized by its lush vegetation, and for having soils with a superficial horizon rich in OM. These data led us to mistakenly think that they were fertile lands. After several investigations in deforested areas, it has been proven that Amazonian lands are not very fertile. They become impoverished quickly in the absence of organic remains provided by the vegetation cover . In Amazonian soils, despite the low availability of nutrients, forests have adapted to weathering and washing conditions, capturing the nutrients generated by the decomposition of the OM directly with their surface roots .
In Ecuador, oil activity is indirectly responsible for the current state of Amazonian forests . With the appearance of oil companies, agricultural and livestock activities have positioned themselves as being the main and majority use of the Amazon region . Currently, institutions and research centers are developing projects aimed at the sustainable use of natural resources . These projects are associated with environmentally friendly management systems and practices that benefit the Amazonian community.
In the Ecuadorian Amazon, most forests that are not part of the National System of Protected Areas of Ecuador (SNAP) or some protective forest are degraded either by the selective extraction of species or because they are secondary forests .