- Please check and comment entries here.
Platelet concentrates (PCs) typically refer to a group of materials produced from autologous blood designed to improve tissue regeneration.
1. Overview of Lyophilization for Platelet Concentrates
Lyophilization, or freeze-drying, has been developed as a method for transforming solutions containing labile substances into more stable solids to enable their distribution as well as preserving bioactivity for different applications. This process is widely used to enhance the stability and long-term storage of proteins in the pharmaceutical, biotechnology and food industries. Indeed, freeze-drying offers storage and processing benefits over conventional methods, including (1) longer storage times at room temperature; (2) rapid transformation by rehydration, which enables practical application in emergency medicine; and (3) increased stability to enable transport and application at distant sites.
The idea of platelet lyophilization was originally proposed by Wolkers, who initially indicated that freeze-dried platelets functioned biologically similarly to endogenous platelets. Indeed, these lyophilized platelets not only exhibit increased storage stability but are also able to rapidly release bioactive growth factors, such as platelet-derived growth factor-BB (PDGF-BB), TGF-β1, and VEGF at the surgical site. It does not have a detrimental effect on PCs’ ability to facilitate tissue regeneration, suggesting that a range of cytokines and fibrin networks in the PCs are preserved and have the capacity to promote chemotaxis and cell proliferation. LPCs express their growth factors more slowly than PCs. This was discovered in a study by Zheng when they attempted to combine nano-hydroxyapatite (nHA), L-lactic acid-co-glycolic acid (PLGA), and the hydrogel (nHA/PLGA/Gel) scaffold with LPCs. The in vitro release experiments showed that the composite scaffold allowed for gradual and continuous release of PRF-derived growth factors. They examine the pattern of growth factors released from the composite scaffolds over 12 weeks and illustrated that the concentration of IGF-I, TGF-β1, and PDGF-AB in the four weeks reached 66%, 67%, and 65%, respectively. It was demonstrated that there was a higher release rate in the first 4 weeks followed by a relatively steady rate over the following weeks. Figure 1 provides a schematic diagram of the method used for producing lyophilized PCs, and Figure 2 provides a physical comparison of PCs and lyophilized PCs.
Figure 1. Schematic diagram illustrating the processing and development of lyophilized platelet concentrate (LPC) in the form of platelet-rich fibrin (PRF).
Figure 2. Physical comparison of traditionally prepared platelet concentrates (a) versus lyophilized platelet concentrates (b).
The advantages of lyophilized platelet concentrates are further highlighted in a study that recently documented lyophilized PCs exhibiting enhanced osteogenic capability and improved tissue compatibility at the injury site, compared with freshly isolated PCs.
2. Lyophilized Platelet Concentrates in Craniofacial Tissue Regeneration
The use of PCs to deliver growth factors to defective areas to stimulate tissue regeneration in both the medical and dental fields has gained considerable interest over recent years. However, despite the considerable advantages offered by LPCs, only a limited number of studies, summarized below, have been reported. This review reports on the current application and potential of LPCs in craniofacial tissue regeneration. Table 1 provides details of studies reporting the different preparation methods of LPCs for potential use as craniofacial scaffolds for tissue regeneration.
|No.||Type||Platelet Concentrate Preparation Protocol||Fabrication of Composite Scaffold with Lyophilized Platelet Concentrates||Scaffold||Cell Type||Animal Model||In Vitro/In Vivo Analysis Method||Main Findings||Author, Year, References|
|1.||PC||10 mL of plasma centrifuged at 4450 rpm for 10 min.||The PC solutions were frozen for one hour in a −20 °C freezer and then for 2 h in a −80 °C until 24 h freezing. Then, the fabricated FDPC powder was combined with the chitosan mixture and β-GP.||Thermo-sensitive chitosan/β-glycerol phosphate (β-GP) hydrogel.||PDLSCs||NA||In vitro||FDPC-loaded hydrogel groups show two weeks of continuous release of TGF-β1 and PDGF-BB. The growth factor release profiles exhibited a similar pattern.||Ammar 2018 |
|2.||A-PRF||10 mL of blood centrifuged at 1500 rpm for 14 min.||The solutions for collagen and chitosan were blended and cross-linking before being agitated for 24 h. At −80 °C, the PC was frozen and dried at −40 °C for 24 h. The lyophilized PC was supplemented to the solution, immediately cast, frozen, and freeze-dried.||Collagen–chitosan membrane with Lyophilized A-PRF.||MSCs||NA||In vitro||A-PRF lowered the rate of degradation and Young’s modulus of the scaffold. A-PRF induced better cell viability and osteogenic differentiation compared to the control group.||Ansarizadeh 2019 |
|3.||PRP||Blood centrifuged at 2400 and 3600 rpm for 10 and 15 min.||For 5 min, PCL scaffolds were submerged in PRP at RT and then stored at −80 °C for 30 min. The frozen samples were immediately freeze-dried. Pending use, the FD-PRP-PCL scaffold was stored at 4 °C.||Traditional
PRP–PCL scaffolds, bare PCL scaffolds and, the freeze-dried PRP–PCL scaffolds.
|DPSCs||Rats||In vitro In vivo||FD-PRP stimulated ALP, RUNX2, OCN and OPN mRNA expression. Scaffolds of the FD-PRP-PCL caused more significant bone formation.||Li 2017 |
|4.||PRP||NA||A collagen sponge was dipped in PRP. The PRP-absorbed collagen sponge was frozen for 60 min at −75 °C and freeze-dried later. It was then kept at 4 °C until used.||FD-PRP-coated collagen sponge with a non-FD-PRP coated collagen sponge.||hAPCs||Mice||In vitro In vivo||PRP-coated sponge failed to induce hAPC proliferation. PRP-coated sponge rapidly caused angiogenesis and the invasion of the connective tissue around it.||Horimizu 2013 |
|5.||PRP||8.5 mL of blood centrifuged at 2400 rpm (103 g) and 3600 rpm (230 g) for 10 and 15 min.||GEL scaffold: PRP was added to chitosan gel and then freeze-dried. SPONGE scaffold: PRP was implanted to freeze-dried chitosan scaffolds using a micropipette.||GEL and SPONGE chitosan scaffold.||NA||NA||In vitro||In the GEL group, a continuous release of GFs was achieved, while a rapid burst release was detected in the SPONGE groups. GEL scaffolds had their porous structure preserved. The GEL scaffold is superior to the SPONGE scaffold because of the morphological architecture of the scaffold.||Kutlu 2013 |
PC: platelet concentrates; FDPC: freeze-dried platelet concentrate; β-GP: β-glycerol phosphate; PDLSCs: periodontal ligament stem cells; TGF-β1: transforming growth factor β-1; PDGF-BB: platelet-derived growth factor-BB; A-PRF: advanced platelet-rich fibrin; MSCs: mesenchymal stem cells; PRP: platelet-rich plasma; PCL: polycaprolactone; FD-PRP: freeze-dried platelet-rich plasma; FD-PRP-PCL: freeze-dried platelet-rich plasma and polycaprolactone; DPSCs: dental pulp stem cells; ALP: alkaline phosphatase; RUNX2: runt-related gene-2; OCN: osteocalcin; OPN: osteopontin; PDLSCs: periodontal ligament stem cells; hAPCs: human alveolar periosteal cells; GFs: growth factors; NA: not available.
Periodontitis is an important disease in dentistry that can result in both hard and soft tissue loss. Periodontal regeneration and healing can be enhanced by utilizing growth factors that modulate cellular responses, and, therefore, LPCs have been incorporated into chitosan hydrogel scaffolds containing encapsulated periodontal ligament stem cells (PDLSCs). PDLSC proliferation and migration rate increased dramatically with LPC inclusion as compared with fresh PC-containing hydrogel controls. This outcome was reportedly potentially due to both the increased pore diameter in the LPC containing scaffolds as well as enhanced growth factor release.
For several decades, barrier membranes have been used in oral and maxillofacial surgery. The most popular materials for the manufacture of these membranes are collagen, fresh PCs, and chitosan compounds. Recently, Ansarizadeh reported the integration of chitosan and LPCs into a composite collagen membrane, and the results revealed that both the chitosan/collagen weight percentage and the concentration of LPCs enhanced mechanical strength and membrane biodegradability. Furthermore, increased cell proliferation, higher cell viability, and osteogenic differentiation were enabled by the incorporation of LPCs.
LPCs have also been assessed clinically to determine their applicability as a scaffolding material for other craniofacial tissue regeneration applications. The data demonstrated that constructs exhibited a consistent fibrin structure with a significant leukocyte infiltrate. Transmission electron microscopy (TEM) studies verified the preservation of their potency in promoting tissue healing, even at 1-week following lyophilization at −196 °C. For the assessment of general healing at the surgical site, color, swelling, bleeding, and post-operative pain were assessed to determine the effect of the LPCs. No apparent differences were identified compared with the control group, which was composed of freshly isolated PCs. Consequently, the authors concluded that the LPC constructs promoted bone regeneration and that they also promoted chemotaxis and proliferation of neighboring osteoblast progenitors similarly to the fresh PC isolate control.
Thus far, there have been only a relatively small number of studies analyzing the effects of PCs for pulp regenerative therapies. Two recent reports have, however, aimed at determining the potential application of LPCs in regenerative endodontic procedures. The authors reported that the lyophilization approach still enabled comprehensive local release of cytokines. Xu, conducting a study on beagle dogs, showed that LPC application was capable of inhibiting the release of pro-inflammatory cytokines while promoting proliferation, migration, and odonto-/osteo-genic differentiation of human dental stem pulp cells (hDPSCs). Furthermore, LPCs also induced dentine–pulp complex regeneration as well as enabling continued root growth in the immature teeth treated.
In contrast, a recent study reported that both LPCs and fresh PCs inhibited the mineralization potential during the first week of application. The authors speculated that the stem cells from the apical papilla (SCAP) used in the study were unique compared to other types of mesenchymal stem cells. This is because SCAPs have been certified to regenerate into vascularized dentine–pulp complex in vivo, and it is a stem cell niche that is known to be a source of odontoblasts during dental development. Notably, however, this study still highlighted the potential for application of LPCs in dental tissue engineering, particularly for the treatment of pulp necrosis and periapical periodontitis in immature teeth.
2.1. Craniofacial Wound Healing
The interest in the application of tissue engineering and PC strategies for craniofacial wound repair has increased in interest in recent years. Nakajima published a report on a LPC hybrid scaffold preparation using a polyglactin mesh for use as a wound dressing. One of its drawbacks, however, was the increased host inflammatory response that occurred due to the polyglactin degradation. Consequently, the use of a collagen sponge as a carrier for the LPCs has been explored. It was found that the LPC coating improved the collagen sponge’s ability to attract and become infiltrated by fibroblasts, as well as promoting neo-angiogenesis in the surrounding tissue. The data indicated that the LPC-coated collagen sponge exhibited enhanced wound healing and regenerative capacity by stimulation of angiogenesis and infiltration of cells from surrounding tissue without causing a substantial inflammatory response. Furthermore, in the LPC-coated collagen sponge group, they identified considerably thicker capillary blood vessels compared to the noncoated sponge group at 4 weeks and 12 weeks post-implantation. This result is in agreement with Pietramaggiori, who noted that LPCs facilitated wound healing in a chronic wound model developed in diabetic mice. Interestingly, in contrast to the nontreated group, this study revealed a significant (p < 0.01) fivefold increase in blood vessel density in the LPC group. Recently, Xu et al.suggested that the vascularization process was accelerated in their skin wound study in mice after using a scaffold composed of polyvinyl alcohol (PVA) hydrogel and LPCs as a dressing within 9 days post-surgery. Hence, they believe the neo-angiogenesis could provide oxygen and nutrient to the surgical site, thus promoting protein synthesis.
2.2. Lyophilized Platelet Concentrates as a Craniofacial Bioactive Scaffold
Guided tissue repair approaches are dependent upon growth factor activity. Incorporating PCs into a carrier system, such as a 3D scaffold, can enable controlled release of these molecules as well as enhancing their bioavailability. Kutlu  demonstrated that a chitosan scaffold loaded with PCs provided an excellent tool for multiple concurrent releases of PC-derived growth factors, and hence recommended its use in tissue repair purposes. Further research has assessed the applicability of LPCs as a scaffold for regeneration of craniofacial tissue and compared their biological effects with fresh PCs. Dental follicle cells, periodontal progenitor cells, and alveolar bone cells were used in comparisons of their cellular activity in response to fresh and lyophilized PCs. The data indicated that LPCs exhibited superior effects compared with fresh PCs as a scaffolding material in terms of induction of proliferation, osteogenic differentiation, and tissue integration. The authors concluded that the LPC preparations not only increased the capacity of cells to migrate and proliferate within the scaffold due to an increased pore size, but the construct also enabled a gradual release of growth factors, such as TGF-β1, PDGF, and VEGF from the biomaterial surface. Similarly, Liumore recently reported that the lyophilization process enhanced the fibrin and platelet structures, enabling improved bioactivity.
As LPCs reportedly continuously release bone regenerating growth factors, Li and colleagues compared the utility of 3D-printed polycaprolactone (PCL) scaffolds containing fresh PCs and LPCs for bone repair. The LPC–PCL scaffold exhibited superior stimulation of bone growth as compared with the conventional PC–PCL scaffold. Furthermore, their results indicated that in vivo, mineralization and osteogenesis could be promoted by coating 3D-printed PCL scaffolds with LPCs. The authors postulated that this outcome could be directly linked to the sustained release of PC-derived growth factors, including VEGF, PDGF, basic fibroblast growth factor (bFGF), TGF-β1, EGF, and IGF-1. Table 2 summarizes the in vitro, in vivo and one randomized clinical trial application of LPCs to enhance tissue regeneration. Table 3 summarises the strengths and limitations of LPC.
|No.||Type||Platelet Concentrate Preparation Protocol||Lyophilization Method||Comparison Group||Cell Type||Animal Model||Type of Study||Main Finding||Author, Year, References|
|1.||PRF||8 mL blood centrifuged at 1700 rcf for 5 min.||The PRF membrane was frozen for 30 min at −80 °C and freeze-dried overnight (−54 °C, 12 Pa).||Fresh PRF and frozen PRF||MSCs, HGFs||NA||In vitro||In FD-PRF, the proliferation of MSCs was greater. Frozen PRF and FD-PRF were more compact and had a rough texture. Frozen PRF had lower activity in plasmin.||Kardos 2018 |
|2.||PRF||10mL blood centrifuged at 2100 rpm (400 g) for 12 min.||The frozen PRF membranes were kept at −80 °C. The frozen PRF was then freeze-dried at −51 °C overnight.||Fresh PRF||DFs, Abs, PDLs||Rats||In vitro In vivo||L-PRF caused the proliferation and migration of the PDL cells.
In AB cells, L-PRF stimulated RUNX2. L-PRF protected 97% of bone defects compared to 84% in the case of fresh PRF.
|Li 2014 |
|3.||CGF and PRF||10 mL of blood.||In a vacuum freeze dryer, the CGF and PRF membranes were frozen overnight.||FD-PRF and FD-CGF||SCAPs||NA||In vitro||Major growth rate and migratory cells in FD-CGF and FD-PRF groups. After 7 days and 14 days, substantial mineralized areas in FD-CGF and FD-PRF.||Hong 2018 |
|4.||CGF||10 mL blood centrifuged for 2 min at 2700 rpm (600 g), 4 min at 2400 rpm (400 g), 4 min at 2700 rpm (600 g), and 3 min at 3000 rpm.||The isolated CGF membranes were frozen in a vacuum freeze dryer overnight.||NA||hDPSCs||Beagle dogs||In vitro In vivo||CGF had a protective effect on the inflamed hDPSCs. CGF had a strong impact on hDPSC proliferation, migration. and differentiation. CGF facilitated complex regeneration of the dentine pulp in immature teeth.||Xu 2019 |
|5.||L-PRF||10 mL of blood centrifuged at 3000 rpm (400 g) for 10 min.||5% DMSO and PRF were freeze-dried for 24 h at −80 °C and cryopreserved for a week in the −196 °C. L-PRF was thawed for 3 min, then rinsed with PBS two to three times. Later, L-PRF was fixed in 4% paraformaldehyde.||Fresh PRF and L-PRF were implanted into the patient’s edentulous anterior maxillary region for GBR.||NA||NA||Clinical trial||Fresh PRF and L-PRF illustrated clinical and immunohistochemical similarities. L-PRF growth factors and fibrin networks were able to facilitate chemotaxis and the proliferation of adjacent osteoblasts.||Zhang 2017 |
|Strength||1. Preservation of biological properties;|
|2. Preservation of morphological architecture;|
|3. Sustained release of growth factors;|
|4. 100% natural and autologous;|
|5. Biocompatible with other biomaterials;|
|6. Multiple usages with single venipuncture;|
|7. Easy Transportation;|
|8. Better storage capabilities;|
|9. Enables use in emergency surgery;|
|10. Longer clinical shelf-life.|
|Limitation||1. Fabrication cost;|
|2. Possible risk of contamination;|
|3. Demands standardization protocol for lyophilization technique.|
2.3. Limitations of the Study
The dynamic biology of PCs and their role in tissue regeneration and inflammation have provided the basis for PC and LPC therapies for a wide variety of medical and dental treatments. However, scientific advancements remain hindered by the lack of standardization of PC and LPC products, doses, and fabrication protocols. The situation continues to be ambiguous as the different approaches and materials used do not seem to produce the same material as original PCs. Moreover, heterogeneous processing methods, unstandardized nomenclature, and vague classifications complicate comparisons among studies; thus, we recommend a comprehensive, detailed and step-by-step explanation of the preparation procedure for the LPC to allow for comparison between studies, ensuring reproducibility to prevent misunderstanding and misleading assumptions in the literature (Table 3).
This entry is adapted from 10.3390/molecules26030517
- Anitua, E.; Sánchez, M.; Orive, G.; Andía, I. The potential impact of the preparation rich in growth factors (PRGF) in different medical fields. Biomaterials 2007, 28, 4551–4560.
- Dohan, D.M.; Choukroun, J.; Diss, A.; Dohan, S.L.; Dohan, A.J.; Mouhyi, J.; Gogly, B. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part II: Platelet-related biologic features. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 2006, 101, e45–e50.
- Zhang, L.; Ai, H. Concentrated growth factor promotes proliferation, osteogenic differentiation, and angiogenic potential of rabbit periosteum-derived cells in vitro. J. Orthop. Surg. Res. 2019, 14, 1–10.
- Foster, T.E.; Puskas, B.L.; Mandelbaum, B.R.; Gerhardt, M.B.; Rodeo, S.A. Platelet-rich plasma: From basic science to clinical ap-plications. Am. J. Sports Med. 2009, 37, 2259–2272.
- Marx, R.E.; Carlson, E.R.; Eichstaedt, R.M.; Schimmele, S.R.; Strauss, J.E.; Georgeff, K.R. Platelet-rich plasma: Growth factor en-hancement for bone grafts. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 1998, 85, 638–646.
- Choukroun, J.; Diss, A.; Simonpieri, A.; Girard, M.-O.; Schoeffler, C.; Dohan, S.L.; Dohan, A.J.; Mouhyi, J.; Dohan, D.M. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part IV: Clinical effects on tissue healing. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 2006, 101, e56–e60.
- Huber, S.C.; Cunha, J.L.; Montalvao, S.A.L.; da Silva, L.Q.; Paffaro, A.U.; da Silva, F.A.R.; Rodrigues, B.L.; Santos Duarte Lana, J.F.; Annichino-Bizzacchi, J.M. In vitro study of the role of thrombin in platelet rich plasma (PRP) preparation: Utility for gel formation and impact in growth factors release. J. Stem Cells Regen. Med. 2016, 12, 2–9.
- Miron, R.J.; Choukroun, J. Platelet Rich Fibrin in Regenerative Dentistry; John Wiley & Sons Ltd.: Hoboken, NJ, USA, 2017.
- Choukroun, J.; Diss, A.; Simonpieri, A.; Girard, M.-O.; Schoeffler, C.; Dohan, S.L.; Dohan, A.J.; Mouhyi, J.; Dohan, D.M. Platelet-rich fibrin (PRF): A second-generation platelet concentrate. Part V: Histologic evaluations of PRF effects on bone allograft maturation in sinus lift. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 2006, 101, 299–303.
- Jeon, Y.R.; Kim, M.J.; Kim, Y.O.; Roh, T.S.; Lee, W.J.; Kang, E.H.; Yun, I.S. Scaffold Free Bone Regeneration Using Platelet-Rich Fibrin in Calvarial Defect Model. J. Craniofac. Surg. 2018, 29, 251–254.
- Dohan Ehrenfest, D.M.; Diss, A.; Odin, G.; Doglioli, P.; Hippolyte, M.-P.; Charrier, J.-B. In vitro effects of Choukroun’s PRF (platelet-rich fibrin) on human gingival fibroblasts, dermal prekeratinocytes, preadipocytes, and maxillofacial osteoblasts in primary cultures. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 2009, 108, 341–352.
- Yung, Y.-L.; Fu, S.-C.; Cheuk, Y.-C.; Qin, L.; Ong, M.T.-Y.; Chan, K.-M.; Yung, P.S. Optimisation of platelet concentrates therapy: Composition, localisation, and duration of action. Asia Pac. J. Sports Med. Arthrosc. Rehabil. Technol. 2017, 7, 27–36.
- Zheng, L.; Wang, L.; Qin, J.; Sun, X.; Yang, T.; Ni, Y.; Zhou, Y. New Biodegradable Implant Material Containing Hydrogel with Growth Factors of Lyophilized PRF in Combination with an nHA/PLGA Scaffold. J. Hard Tissue Biol. 2015, 24, 54–60.
- Horimizu, M.; Kawase, T.; Nakajima, Y.; Okuda, K.; Nagata, M.; Wolff, L.F.; Yoshie, H. An improved freeze-dried PRP-coated biode-gradable material suitable for connective tissue regenerative therapy. Cryobiology 2013, 66, 223–232.
- Xu, F.; Zou, D.; Dai, T.; Xu, H.; An, R.; Liu, Y.; Liu, B. Effects of incorporation of granule-lyophilised platelet-rich fibrin into pol-yvinyl alcohol hydrogel on wound healing. Sci. Rep. 2018, 8, 14042.
- Nireesha, G.; Divya, L.; Sowmya, C.; Venkateshan, N.; Babu, M.; Lavakumar, V. Lyophilization/Freeze Drying—A Review. Int. J. Nov. Trends Pharm. Sci. 2013, 3, 87–98.
- Li, Q.; Reed, D.A.; Min, L.; Gopinathan, G.; Li, S.; Dangaria, S.J.; Li, L.; Geng, Y.; Galang-Boquiren, M.T.; Gajendrareddy, P.; et al. Lyophilized Platelet-Rich Fibrin (PRF) Promotes Craniofacial Bone Regeneration through Runx. Int. J. Mol. Sci. 2014, 15, 8509–8525.
- Pan, L.; Yong, Z.; Yuk, K.S.; Hoon, K.Y.; Yuedong, S.; Xu, J. Growth Factor Release from Lyophilized Porcine Platelet-Rich Plasma: Quantitative Analysis and Implications for Clinical Applications. Aesthetic Plast. Surg. 2015, 40, 157–163.
- Valeri, C.R.; Feingold, H.; Marchionni, L.D. A Simple Method for Freezing Human Platelets Using 6% Dimethylsulfoxide and Storage at −80 °C. Blood 1974, 43, 131–136.
- Read, M.S.; Reddick, R.L.; Bode, A.P.; Bellinger, D.A.; Nichols, T.C.; Taylor, K.; Smith, S.V.; McMahon, D.K.; Griggs, T.R.; Brinkhous, K.M. Preservation of hemostatic and structural properties of rehydrated lyophilized platelets: Potential for long-term storage of dried platelets for transfusion. Proc. Natl. Acad. Sci. USA 1995, 92, 397–401.
- Fan, J.-L.; Xu, X.-G.; Zhang, S.-Z.; Zhu, F.-M.; Chen, G.-M.; Yan, L.-X. Experimental study on rehydration conditions of freeze-dried platelets. J. Zhejiang Univ. Sci. A 2009, 10, 697–703.
- Muraglia, A.; Ottonello, C.; Spanò, R.; Dozin, B.; Strada, P.; Grandizio, M.; Cancedda, R.; Mastrogiacomo, M. Biological activity of a standardized freeze-dried platelet derivative to be used as cell culture medium supplement. Platelets 2014, 25, 211–220.
- Wolkers, W.F.; Walker, N.J.; Tablin, F.; Crowe, J.H. Human Platelets Loaded with Trehalose Survive Freeze-Drying. Cryobiology 2001, 42, 79–87.
- Nakatani, Y.; Agata, H.; Sumita, Y.; Koga, T.; Asahina, I. Efficacy of freeze-dried platelet-rich plasma in bone engineering. Arch. Oral Biol. 2017, 73, 172–178.
- Sum, R.; Hager, S.; Pietramaggiori, G.; Orgill, D.P.; Dee, J.; Rudolph, A.; Orser, C.; Fitzpatrick, G.M.; Ho, D. Wound-healing properties of trehalose-stabilized freeze-dried outdated platelets. Transfusion 2007, 47, 672–679.
- Vocetkova, K.; Buzgo, M.; Sovkova, V.; Rampichová, M.; Staffa, A.; Filova, E.; Lukasova, V.; Doupnik, M.; Fiori, F.; Amler, E. A comparison of high throughput core–shell 2D electrospinning and 3D centrifugal spinning techniques to produce platelet lyophilisate-loaded fibrous scaffolds and their effects on skin cells. RSC Adv. 2017, 7, 53706–53719.
- Zhang, J.; Qi, X.; Luo, X.; Li, D.; Wang, H.; Li, T. Clinical and immunohistochemical performance of lyophilized platelet-rich fibrin (Ly-PRF) on tissue regeneration. Clin. Implant. Dent. Relat. Res. 2017, 19, 466–477.
- Ammar, M.M.; Waly, G.H.; Saniour, S.H.; Moussa, T.A. Growth factor release and enhanced encapsulated periodontal stem cells viability by freeze-dried platelet concentrate loaded thermo-sensitive hydrogel for periodontal regeneration. Saudi Dent. J. 2018, 30, 355–364.
- Ansarizadeh, M.; Mashayekhan, S.; Saadatmand, M. Fabrication, modeling and optimization of lyophilized advanced platelet rich fibrin in combination with collagen-chitosan as a guided bone regeneration membrane. Int. J. Biol. Macromol. 2019, 125, 383–391.
- Li, J.; Chen, M.; Wei, X.; Hao, Y.; Wang, J. Evaluation of 3D-Printed Polycaprolactone Scaffolds Coated with Freeze-Dried Platelet-Rich Plasma for Bone Regeneration. Materials 2017, 10, 831.
- Kutlu, B.; Aydın, R.S.T.; Akman, A.C.; Gümüşderelioğlu, M.; Nohutcu, R.M. Platelet-rich plasma-loaded chitosan scaffolds: Preparation and growth factor release kinetics. J. Biomed. Mater. Res. Part B Appl. Biomater. 2012, 101, 28–35.
- Kardos, D.; Hornyák, I.; Simon, M.; Hinsenkamp, A.; Marschall, B.; Várdai, R.; Menyhárd, A.; Pinke, B.; Mészáros, L.; Kuten, O.; et al. Biological and Mechanical Properties of Platelet-Rich Fibrin Membranes after Thermal Manipulation and Preparation in a Single-Syringe Closed System. Int. J. Mol. Sci. 2018, 19, 3433.
- Hong, S.; Chen, W.; Jiang, B. A Comparative Evaluation of Concentrated Growth Factor and Platelet-rich Fibrin on the Proliferation, Migration, and Differentiation of Human Stem Cells of the Apical Papilla. J. Endod. 2018, 44, 977–983.
- Xu, F.; Qiao, L.; Zhao, Y.; Chen, W.; Hong, S.; Pan, J.; Jiang, B. The potential application of concentrated growth factor in pulp re-generation: An in vitro and in vivo study. Stem Cell Res. Ther. 2019, 10, 134.
- Nakajima, Y.; Kawase, T.; Kobayashi, M.; Okuda, K.; Wolff, L.F.; Yoshie, H. Bioactivity of freeze-dried platelet-rich plasma in an adsorbed form on a biodegradable polymer material. Platelets 2012, 23, 594–603.
- Pietramaggiori, G.; Kaipainen, A.; Czeczuga, J.M.; Christopher, T.W.; Orgill, D.P. Freeze-dried platelet-rich plasma shows beneficial healing properties in chronic wounds. Wound Repair Regen. 2006, 14, 573–580.
- Liu, Z.; Jin, H.; Xie, Q.; Jiang, Z.; Guo, S.; Li, Y.; Zhang, B. Controlled Release Strategies for the Combination of Fresh and Lyophilized Platelet-Rich Fibrin on Bone Tissue Regeneration. BioMed Res. Int. 2019, 2019, 4923767.
- Andia, I.; Perez-Valle, A.; Del Amo, C.; Maffulli, N. Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization. Int. J. Mol. Sci. 2020, 21, 6904.
- Dohan Ehrenfest, D.M.; Pinto, N.R.; Pereda, A.; Jiménez, P.; Corso, M.D.; Kang, B.-S.; Nally, M.; Lanata, N.; Wang, H.-L.; Quirynen, M. The impact of the centrifuge characteristics and centrifugation protocols on the cells, growth factors, and fibrin architecture of a leukocyte- and platelet-rich fibrin (L-PRF) clot and membrane. Platelets 2017, 29, 171–184.
- Miron, R.J.; Pinto, N.R.; Quirynen, M.; Ghanaati, S. Standardization of relative centrifugal forces in studies related to plate-let-rich fibrin. J. Periodontol. 2019, 90, 817–820.
- Chahla, J.; Cinque, M.E.; Piuzzi, N.S.; Mannava, S.; Geeslin, A.G.; Murray, I.R.; Dornan, G.J.; Muschler, G.F.; LaPrade, R.F. A Call for Standardization in Platelet-Rich Plasma Preparation Protocols and Composition Reporting: A Systematic Review of the Clinical Orthopaedic Literature. J. Bone Jt. Surg. Am. 2017, 99, 1769–1979.