Are Leukaemic Stem Cells Restricted to a Single Cell Lineage?
Hematopoietic stem cells ‘choose’ a cell lineage from all options to develop towards a mature blood cell type and they are a mixture of cells with different lineage signatures. However, hematopoietic stem cells and their progeny retain enough versatility to ‘step sideways’ into a different pathway. Most, if not all cancers, arise from tissue-specific stem cells that have accumulated oncogenic insults to their genome and epigenome. We believe that the first oncogenic insult to a normal hematopoietic stem cell restricts the resulting leukaemia stem cell and its progeny to a single pathway. We hypothesized this from studies of transgenic mice in which we restricted oncogene expression to hematopoietic stem cells, via the Sca-1 promotor, and this led to lineage-restricted leukaemias.
The precise cellular origin of a cancer, and the ability of the underlying cell to differentiate dictate the course of overt disease. This, in turn, influences how well patients respond to their treatment. The specific type of cell in which a cancer originates, the cancer-initiating cell (CIC), has been of longstanding interest to scientists and clinicians. Even now, the identity of this cell is unclear in some cancers. In the late 1990s, John Dick and his colleagues proposed cancer-stem-cell theory on the basis of evidence from studies of acute myeloid leukaemia (AML). They stated that most, if not all, cancers arise from genetic lesions involving a tissue-specific stem cell []. In the case of AML, the leukaemia-initiating cell (LIC) was a pluripotent haematopoietic stem cell (HSC). John Dick’s proposal was keeping with the view that HSCs and their progeny (haematopoietic progenitor cells, HPCs) were prime targets for malignant transformation due to their inherent properties of self-renewal, long life, and proliferative potential. To this day, investigators also view mature B and T lymphocytes as targets for malignancy by virtue of their proliferative potential [].
There are two key cells with regard to the development of cancer, CICs and cancer stem cells (CSCs). These cells are often confused. Some 100 years ago, Boveri observed chromosomal abnormalities in cancer cells []. Investigators identified further lesions in the 1950s and 1960s, leading to the view that the underlying driver of the development of cancer was an insult to the genome. Around this time, cancer modelling also began to include the concept of cancer initiation (preneoplasia) in addition to overt disease (malignancy). Subsequently, elegant studies of identical twins who had both developed acute lymphoblastic leukaemia (ALL) in childhood revealed that at least two insults to the genome were required for the development of leukaemia []. This was in part due to the intraplacental sharing of a preleukaemic clone containing an identical chromosomal translocation. However, disease occurrence is asynchronous, and therefore requires one prenatal and one (or more) postnatal genomic ‘hits’. The first ‘hit’ converts the LIC, thought to be a B-lymphocyte-committed progenitor in the case of ALL (see below), into a dormant preleukaemic cell. A further ‘hit’ then converts this cell into a leukaemic stem cell (LSC) that gives rise to and sustains the disease.
Nature of Cancer Stems Cells and Their Progeny
A clear picture of the behaviour of LICs/CICs and LSCs/CSCs is important to finding better treatments for malignancies. This is because LSCs are mostly responsible for untreatable disease relapses in AML, and CSCs underlie carcinoma relapse and metastasis. The elimination of CSCs might provide a means to find a cure for carcinomas that are difficult to treat. However, we do not currently have a clear picture of how two or more ‘hits’ transform a normal stem cell into a cancer cell. For example, we do not know if there is a difference between LIC and normal HSC behaviour in childhood ALL. Analysis of leukaemic cells at the time of patient presentation does not provide direct insight into the nature of LICs because the disease process begins many years prior, and the mutational burden is then too high.
CSCs constitute a small fraction of a patient’s cancer cells. The percentage ranges from very small to up to 25% for different types of cancers [], meaning that more mature progeny form the bulk of a patient’s cancer cells. Investigations of HSCs and haematopoiesis have provided the current paradigm of normal stem-cell development and the nature of their progeny. Many longstanding and classical models of haematopoiesis depict a binary cell-fate tree in which the progeny of HSCs and HPCs progressively restrict their developmental options to give rise to end-cell types (see below). Investigators also use this type of model to describe the development of other tissue-specific stem cells. Human AML is composed of a hierarchy of cells that originate from a transformed HSC [] that has differentiated to variable degrees to make up the bulk of a patient’s leukaemic cells.
The behaviour of the bulk of a patient’s cancer cells might elucidate the difference between normal stem cells and CSCs. An early view on the nature of cancer cells arose from the first descriptions of patients with leukaemia by Bennet and Virkow. They reported a high density of white cells in blood and concluded that leukaemic cells divide extensively and/or quickly, and that the abundance of cells might be a response to infection. Rapid cell division at diagnosis is rare because many cancers are indolent, meaning they take time to become evident. The combined ‘hits’, therefore, do not necessarily confer a growth advantage to CICs/CSCs. Though cancers originate from a single transformed cell, the progeny are dynamic and undergo clonal evolution that has major implications for curing advanced cancers. Despite their diversity, cancer cells do not appear to be better equipped to survive. This was shown in a study where cancer subclones seldom demonstrated survival advantages over one another at the time of patient diagnosis []. The differentiation of cancer cells is often perturbed, which has led to the view that there is a skewing of cellular controls to favour proliferation over maturation. However, given that there are numerous factors controlling proliferation and maturation within cells [], a proliferation increase at the expense of maturation is perhaps not a hallmark feature of cancer. Strikingly, leukaemic and carcinoma cells, including those arising from a stem cell, generally belong to one cell lineage. The following review examines whether this is a cardinal feature of cancer.
Leukaemic Cells Belong to One Lineage
The nature of a patient’s cells in chronic myeloid leukaemia (CML) clearly illustrates that an LSC can ‘dump’ cells down one lineage pathway. CML was shown to be a disease of HSCs by Fialkow, who used cells from female patients and X-linked glucose-6-phosphate to reveal that a wide spectrum of blood cell types belong to a leukaemic clone []. Despite this finding, patients’ cells were substantially restricted to the neutrophil pathway. Similarly, acute erythroid leukaemia is an HSC disease, and ≥80% of bone marrow cells are erythroid precursors [].
Cell surface markers, particularly those identified by monoclonal antibodies, have provided a vital tool to determine the precise nature of leukaemic cells. Owing to these markers, an understanding of cell-lineage affiliation has advanced far more in leukaemia than in some other cancers. However, for some human leukaemia types, the common belief about a cell of origin is at odds with experiment evidence that supports earlier HSC origin. As mentioned above, ALL arises from a B-lymphocyte-committed progenitor []. In contrast, HSC-like cells that express marker CD34+ and lack B-lineage markers recreate the disease in mice []. We view acute promyelocytic leukaemia (APL) as a myeloid progenitor malignancy [], with its hallmark feature being a t(15;17) chromosomal translocation giving rise to the PML-RARα oncoprotein. The translocation and oncoprotein are both present in CD34+CD38– HSC-like cells in patients with APL []. It has been a longstanding belief that chronic lymphocytic leukaemia (CLL) is a malignancy of small B-lymphocytes. However, abnormal expression of lymphoid genes in patients’ HSCs, and the fact that HSCs purified from patients with CLL produce a high number of polyclonal B-cell progenitors, indicate an HSC origin [].
Coupled to the assertion that leukaemic cells belong to one cell lineage, as defined by histological features and immunological markers, is that unique chromosomal abnormalities are also present. As stated above, the bulk of CML patients’ cells are relatively mature neutrophils. Hungerford and Novell described a specific chromosomal abnormality that is associated with CML—a small version of chromosome 21, termed the Philadelphia chromosome []. Since this discovery, the development of a high-resolution technique for chromosomal analysis and fluorescence in situ hybridisation (FISH) has led to the discovery of many other chromosomal abnormalities that result in hybrid genes consisting of portions of two different genes. In some cases, multiple different types of cancer express the same hybrid gene, indicating that these genes can play a role in the cancers of different tissue types. Like CML, some chromosomal abnormalities are specifically associated with the histological subtype of a malignancy, such as those seen in non-Hodgkin’s lymphoma. Specifically, a translocation between chromosomes 18 and 14 is seen in patients with follicular lymphoma, and a translocation between chromosomes 8 and 14 is seen in patients with small-noncleaved-cell and large immunoblastic lymphoma []. Chromosomal translocations between chromosomes 11 and 14, 11 and 13, and 7 and 11 activate the LMO2 gene, the product of which is a specific marker of T-cell ALL (T-ALL) []. A translocation between chromosomes 12 and 21, creating the ETV6-RUNX1 fusion gene, is associated with B-cell ALL (B-ALL) [].
Investigators have been able to reproduce in mice the genotype–phenotype associations found in some human leukaemias by restricting the expression of a specific oncogene to the stem-/progenitor-cell compartment. Though leukaemia arises in a primitive cell, leukaemic cells belong to a particular cell lineage. Examples of this association in haematopoietic malignancies include BCR-ABLp210 in CML [], MAFB in multiple myeloma [], and BCL6 in B-cell neoplasia []. Examples of this association in solid tumours include EWS-FL1-1 in Ewing sarcoma [5] and SYT-SSX2 in synovial sarcoma []. In these instances, a specific genetic insult to a stem/progenitor cell is associated with a particular cancer. The BCR-ABLp190, LMO2, and BCR-ABLp210 oncogenes play a role in human B-lymphocyte, acute T-lymphoblastic, and chronic myeloid leukaemia, respectively. In transgenic mice in which the expression of BCR-ABLp190, LMO2, and BCR-ABLp210 was restricted to HSCs via the Sca-1 promotor, carcinogenesis was initiated by the oncogenes, and the resultant cancer recapitulated lineage-restricted human disease []. In transgenic mice, the oncogene is solely active within LICs/LSCs and is therefore not essential for the survival and/or proliferation of more mature lineage-affiliated leukaemic cells. An interpretation of these findings is that the oncogene ‘hardwires’ lineage affiliation either throughout or at a particular stage of LSC development, thus restricting the leukaemic cells to that pathway (Figure 1) []. This may occur via the oncogene-mediated priming of the epigenome in cells to adopt a single cell lineage [,].
Figure 1. First oncogenic insult restricts leukaemic stem cells to a single differentiation pathway. BCR-ABLp190, LMO2, and BCR-ABLp210 oncogenes play a role in human B-lymphocyte, acute T-lymphoblastic, and chronic myeloid leukaemia, respectively. Studies of transgenic mice in which investigators used Sca-1 promotor to restrict oncogene expression to haematopoietic stem cells showed that oncogenes initiated leukaemia development and recapitulated lineage-restricted human disease.
While we argue that specific oncogenes/genomic insults to HSCs give rise to a particular lineage-restricted type of leukaemia, there are some caveats to extending this assertion to other types of cancer. Leukaemias could be unique in their specific genomic/epigenetic insults that serve to drive the transformed HSC along a particular developmental pathway. In addition, a specific insult/chromosomal abnormality is not seen in all cancers. This is especially true of solid tumours. We argue that some leukaemia types have an earlier HSC origin than traditionally thought, but that a lineage-committed progenitor cell may be the origin of some solid tumours. In this case, lineage affiliation is equated to the cell of origin, whereby the cell of origin dedifferentiates to regain stemness while retaining a close lineage affiliation. An argument against this view is that committed epithelial cells can give rise to malignant squamous cell carcinomas despite the absence of an oncogene to revert these cells to a stem-cell-like state []. However, it does seem that stem cells are usually the origin of ‘successful’ squamous malignancies.
There are malignancies in which the simultaneous expression of cell surface markers of different cell types confers a mixed lineage status. Coexpression of markers belonging to at least two lineages is seen in mixed-phenotype acute leukaemia (MPAL). This is a rare subgroup of acute leukaemia (2%–5%) in which cells express myeloid and B- or T-lymphoid markers, or myeloid, B-, and T-lymphoid markers together. MPAL might seem to contradict the oncogene-driven ‘hardwiring’ of HSCs to a cell lineage. However, our understanding of MPAL is still very limited because the causative cells are of ambiguous lineage and origin. It is not known whether it is more effective to treat MPAL patients with acute myeloid or acute lymphoid regimens. The surface expression of lineage markers might not reliably define the predominant cell type in MPAL. Indeed, clinicians consider some cases of MPAL to be acute myeloid leukaemia at diagnosis, with the expression of lymphoid markers being due to inappropriate gene expression []. As mentioned above, ETV6-RUNX1 is associated with B-ALL despite blast cells expressing myeloid markers []. The same applies to BCR-ABL190 in B-ALL []. We view both of these leukaemias as being primarily of a B-lineage restricted cell with aberrant gene expression. Other hybrid states include the epithelial–mesenchymal transition, whereby a polarised epithelial cell undergoes changes to assume a mesenchymal-cell phenotype. This is seen in cancer cells at the invasive front of a tumour, where they convert to a mesenchymal phenotype to spread to other organs. We consider this transition somewhat different to the malignant transformation of HSCs because the epithelial–mesenchymal transition occurs during development and adulthood as a means of generating more cells [].
There are leukaemias and solid cancers in which malignant cells are clearly a mixture of more than one cell type. A striking example is the transformation of embryonic stem cells to give rise to teratomas consisting of multiple cell types. This is also the case for the transformation of induced pluripotent cells (iPSCs). iPSC-derived teratomas resemble benign teratomas that occur in mice and humans during early life []. We consider them ‘unsuccessful’ malignancies and excluded them from consideration with regard to lineage fixation. The classification and origin of spontaneous teratomas in humans is complex and unclear. Some types seem to arise from multiple defects during germline development . As such, their development does not seem to be initiated by an oncogene-driven event.
Do Normal Stem Cells Behave Differently?
As mentioned above, investigators have used treelike models for many years to describe the development of HSCs. In recent years, our view has radically changed. We previously viewed HSCs as a pluripotent population of cells. Branch points in treelike models of haematopoiesis dictate routes that individual HSCs follow towards each end-cell fate via a series of intermediate HPCs. The final step involves commitment to a single lineage. However, HSCs selectively express lineage-associated receptors on their surface: for erythropoietin (Epo) [], macrophage colony-stimulating factor (M-CSF) [], granulocyte colony-stimulating factor (G-CSF) [], granulocyte/macrophage colony-stimulating factor (GM-CSF) [], and FMS-like tyrosine kinase 3 ligand (Flt3L) []. In keeping with findings that support the initiation of lineage affiliation earlier than previously thought (Figure 2), recent studies have shown that, within HSCS, human adult bone-marrow CD34+ cells include pluripotent cells and cells with unipotent myeloid or erythroid potential. In this case, the downstream progeny of HSCs that we have routinely considered to be a single population of cells on the basis of cell surface markers could also be a mixture of cells with lineage signatures. This is true for the population of cells first described as early progenitors with lymphoid and myeloid potential (EPLM) []. RNA sequencing of single cells in a ‘primitive’ subpopulation of EPLM that lacked expression of markers Ly6D, SiglecH, and CD11c revealed that the subpopulation was composed of cells with a myeloid, dendritic cell, or lymphoid signature. Few cells had lymphoid and myeloid potential [].
Figure 2. Continuum haematopoiesis model. In our model, haemopoietic stem cells (HSCs) are a mixture of cells with different lineage signatures (shown by different colours) that ‘choose’ a lineage from all options. HSCs and haematopoietic progenitor cells (HPCs) retain enough versatility to ‘step sideways’ into a different pathway. Erythropoietin (Epo), granulocyte colony-stimulating factor (G-CSF) and granulocyte/macrophage colony-stimulating factor (GM-CSF), and macrophage colony stimulating factor (M-CSF) can induce erythroid, neutrophil, and monocyte fates, respectively, within HSCs/HPCs.
Our ‘developmental’ view of haematopoiesis is that an HSC selects one lineage from a continuum of all end-cell options, and then differentiates to that cell type (Figure 2). We provided evidence to support our viewpoint elsewhere []. Though the model shows that affiliation/commitment to a cell lineage can occur as early as during the HSC stage, haematopoiesis still remains dynamic. HSCs and HPCs can still ‘change their mind’ and adopt an alternative fate. Megakaryocyte-primed HSCs can ‘step sideways’ to erythropoiesis, as there is a ‘shared’ trajectory in these pathways due to a common dependence on transcription factor GATA-1 []. Investigators found that cells are able to move to the left or right of a chosen trajectory in the erythroid, granulocytic/macrophage, and lymphoid pathways. They established this by using the RNA sequencing of more than 1600 single HSCs and HPCs to construct expression maps [].
With regard to HPCs, it was shown that mouse progenitor thymocytes that are well on their way to becoming T cells are not firmly restricted to this option—they can still give rise to macrophages, natural killer cells, and B-lymphocytes. The presence of an appropriate cytokine (e.g., M-CSF for macrophages) is essential to divert their fate in vitro (see below) []. Dispersal of HPCs from the bone marrow in a semisolid medium with the addition of particular growth factors (colony-stimulating factors) leads to the production of colonies containing various cell types depending on the added growth factor(s). We argued that the single cell giving rise to the colony ‘shuffles sideways’ to adopt various pathways because it is out of its normal environment with regard to the control of cell fate []. Single-cell analysis of mixed-lineage states has led to the proposal that bursts of alternative gene expression are an important feature of cell decision making, including the existence of bilineage states [].
As mentioned above, the presence of M-CSF is required to derive macrophages from thymocyte progenitors. The selective expression of cytokine receptors by HSCs is relevant to how they ‘choose’ to follow a pathway of development. We formerly viewed all the above cytokines as survival and growth factors for cells that are committed to a certain cell lineage(s). We now know that they also instruct lineage choice. Epo commits multipotent HPCs to erythropoiesis []. M-CSF induces a myeloid lineage fate in HSCs [], and a macrophage fate in granulocyte/macrophage progenitors []. G-CSF and GM-CSF induce the generation of neutrophils from granulocyte/macrophage progenitors (Figure 2) [,]. Flt3L drives the myeloid–lymphoid development of primitive mouse bone-marrow cells, suppressing the generation of megakaryocyte and erythroid progenitors []. Like normal haematopoietic cells, leukaemic cells (e.g., AML cells) depend on the presence of growth factors and an appropriate environment for their survival []. Therefore, we must assume growth factors are readily available. A supply of haematopoietic cytokines may continuously ‘shape’ the production of various types of blood cells to meet the overall demand of an organism. This may include diverting HSCs/HPCs towards a certain cell type to deal with particular circumstances or infectious agents []. For example, HSCs, rather than multipotent HPCs, drive erythropoiesis during chronic erythroid stress in Epo-transgenic mice. These mice demonstrate enhanced cell division, with HSCs exhibiting a committed erythroid progenitor profile [].
Given the above evidence, the first oncogenic insult to a tissue-specific stem cell initiates carcinogenesis by restricting lineage choices of LSCs and other CSCs, and directing their progeny into a single developmental pathway [,]. In this scenario, LSCs/CSCs and their progeny lack the versatility of HSCs/HPCs, which is an essential difference between normal and cancer stem cells. However, several components of this phenomenon are poorly understood. Specifically, the degree to which LSCs/CSCs are restricted is unknown, and, in other words, whether they are restricted from all other fate options. The ability to ‘unlock’ these cells from their fates should also be investigated. As stated above, we now know that Epo, M-CSF, G-CSF, GM-CSF, and Flt3L induce a specific cell lineage. We can use these cytokines to test whether they are able to redirect the differentiation of mouse LSCs in B-lymphocyte and T-lymphocyte leukaemias, and whether Epo can divert LSCs in myeloid leukaemia towards erythropoiesis. In addition, by dissecting how epigenetic priming plays a role in the risk and clinical course of leukaemia, we may be able to intervene by developing epigenetic modifiers. Unlike genetic changes, epigenetic changes can be manipulated in order to treat patients and even before a preleukaemic cell has evolved into leukaemia.
The article has been published on 10.3390/ijms21010045
- John E. Dick; Stem cell concepts renew cancer research. Blood 2008, 112, 4793-4807, 10.1182/blood-2008-08-077941.
- S E Salmon; M Seligmann; B-cell neoplasia in man.. The Lancet 1974, 304, 1230-1233, .
- Nicholas A Wright; Boveri at 100: cancer evolution, from preneoplasia to malignancy. The Journal of Pathology 2014, 234, 146-151, 10.1002/path.4408.
- Mel F. Greaves; Ana Teresa Maia; Joseph L. Wiemels; Anthony M. Ford; Leukemia in twins: lessons in natural history. Blood 2003, 102, 2321-2333, 10.1182/blood-2002-12-3817.
- Bonnet, D.; Dick, J.E. Human acute myeloid leukaemia is organised as a hierarchy that originates from a primitive hematopoietic cell. Nat. Med. 1997, 7, 730–737.
- Quintiana, E.; Shakleton, M.; Sabel, M.S.; Fullen, D.R.; Johnson, T.M.; Morrison, S.J. Efficient tumour formation by single human carcinoma cells. Nature 2008, 456, 593–598.
- Vicente-Duenas, C.; Perez-Caro, M.; Abullo-Jimenez, F.; Cobaleda, C.; Sanchez-Garcia, I.; Stem cell driven cancer: “hands-off” regulation of cancer development. Cell Cycle 2009, 8, 1314–1318, 10.1172/JCI39104C1.
- Lucy R. Yates; Peter J. Campbell; Evolution of the cancer genome.. Nature Reviews Genetics 2012, 13, 795-806, 10.1038/nrg3317.
- Mark T. Drayson; Robert H. Michell; Jennifer Durham; Geoffrey Brown; Cell Proliferation and CD11b Expression Are Controlled Independently during HL60 Cell Differentiation Initiated by 1,25α-Dihydroxyvitamin D3 or All-trans-Retinoic Acid. Experimental Cell Research 2001, 266, 126-134, 10.1006/excr.2001.5200.
- Brown, G.; Hughes, P.J.; Michell, R.H. Cell differentiation and proliferation-simultaneous but independent? Exp. Cell Res. 2003, 291, 282–288.
- Philip J. Fialkow; A. Michael Denman; Robert J. Jacobson; Mark N. Lowenthal; Chronic Myelocytic Leukemia. Journal of Clinical Investigation 1978, 62, 815-823, 10.1172/JCI109193.
- Kowal-Vern, A.; Mazzella, F.M.; Cotelingham, J.D.; Shrit, M.A.; Rector, J.T.; Schumacher, H.R.; Diagnosis andcharacterisation of acute erythroleukaemia subsets by determining the percentages of myeloblasts and proerythroblasts in 69 cases. Am. J. Hematol. 2000, 65, 5–13, .
- M. Greaves; Molecular genetics, natural history and the demise of childhood leukaemia. European Journal of Cancer 1999, 35, 173-185, 10.1016/s0959-8049(98)00433-x.
- C. V. Cox; Allison Blair; A Primitive Cell Origin for B-Cell Precursor ALL?. Stem Cell Reviews and Reports 2005, 1, 189-196, 10.1385/scr:1:3:189.
- Grimwade, D.; Enver, T.; Acute promyelocytic leukaemia: Where does it stem from?. Leukemia 2004, 1, 375–384, .
- Robin H. Edwards; Mariusz A. Wasik; Janet Finan; Roberto Rodriguez; Jonni Moore; Malek Kamoun; Hanna Rennert; Jennifer Bird; Peter C. Nowell; Kevin E. Salhany; et al. Evidence for early hematopoietic progenitor cell involvement in acute promyelocytic leukemia.. American Journal of Clinical Pathology 1999, 112, 819-827, 10.1093/ajcp/112.6.819.
- Yoshikane Kikushige; Fumihiko Ishikawa; Toshihiro Miyamoto; Takahiro Shima; Shingo Urata; Goichi Yoshimoto; Yasuo Mori; Tadafumi Iino; Takuji Yamauchi; Tetsuya Eto; et al. Self-Renewing Hematopoietic Stem Cell Is the Primary Target in Pathogenesis of Human Chronic Lymphocytic Leukemia. Cancer Cell 2011, 20, 246-259, 10.1016/j.ccr.2011.06.029.
- Nowell, P.; Hungerford, D.; A minute chromosome in human granulocytic leukaemia. Science 1960, 132, 1497, .
- Yunis, J.J.; Oken, M.M.; Kaplan, M.E.; Ensrud, K.M.; Howe, R.R.; Theologides, A.; Distinctive chromosomal abnormalities in histologic subtypes of non-Hodgkin’s lymphoma. N. Eng. J. Med. 1982, 307, 1231–1236, .
- Chang-Hoon, N.; Rabbits, T.H.; The role of LMO2 in development and in T cell leukaemia after chromosomal translocation or retroviral insertion. Mol. Ther. 2006, 13, 15–25, .
- S A Shurtleff; A Buijs; F G Behm; J E Rubnitz; S C Raimondi; M L Hancock; G C Chan; C H Pui; G Grosveld; J R Downing; et al. TEL/AML1 fusion resulting from a cryptic t(12;21) is the most common genetic lesion in pediatric ALL and defines a subgroup of patients with an excellent prognosis.. Leukemia 1995, 9, 1985-1989, .
- María Pérez-Caro; César Cobaleda; Inés González-Herrero; Carolina Vicente-Dueñas; Camino Bermejo-Rodríguez; Margarita Sánchez-Beato; Alberto Orfao; Belén Pintado; Teresa Flores; Manuel Sánchez-Martín; et al. Cancer induction by restriction of oncogene expression to the stem cell compartment. The EMBO Journal 2008, 28, 8-20, 10.1038/emboj.2008.253.
- Carolina Vicente-Dueñas; Isabel Romero-Camarero; Inés González-Herrero; Esther Alonso-Escudero; Fernando Abollo-Jiménez; Xiaoyu Jiang; Norma C Gutierrez; Alberto Orfao; Nieves Marín; Luisa María Villar; et al. A novel molecular mechanism involved in multiple myeloma development revealed by targeting MafB to haematopoietic progenitors.. The EMBO Journal 2012, 31, 3704-3717, 10.1038/emboj.2012.227.
- Michael R. Green; Carolina Vicente-Dueñas; Isabel Romero-Camarero; Chih Long Liu; Bo Dai; Ines Gonzalez-Herrero; Idoia Garcia-Ramirez; Esther Alonso-Escudero; Javeed Iqbal; Wing C. Chan; et al. Transient expression of Bcl6 is sufficient for oncogenic function and induction of mature B-cell lymphoma.. Nature Communications 2014, 5, 3904-3904, 10.1038/ncomms4904.
- Nicolò Riggi; Mario-Luca Suvà; Claudio De Vito; Paolo Provero; Jean-Christophe Stehle; Karine Baumer; Luisa Cironi; Michalina Janiszewska; Tanja Petricevic; Mizio Suvà; et al. EWS-FLI-1 modulates miRNA145 and SOX2 expression to initiate mesenchymal stem cell reprogramming toward Ewing sarcoma cancer stem cells. Genes & Development 2010, 24, 916-932, 10.1101/gad.1899710.
- C B Garcia; C M Shaffer; M P Alfaro; A L Smith; J Sun; Z Zhao; P P Young; M N VanSaun; J E Eid; Reprogramming of mesenchymal stem cells by the synovial sarcoma-associated oncogene SYT-SSX2.. Oncogene 2011, 31, 2323-2334, 10.1038/onc.2011.418.
- Alberto Martin-Lorenzo; Franziska Auer; Lai N. Chan; Idoia Garcia-Ramirez; Ines Gonzalez-Herrero; Guillermo Rodríguez-Hernández; Christoph Bartenhagen; Martin Dugas; Michael Gombert; Sebastian Ginzel; et al. Loss of Pax5 Exploits Sca1-BCR-ABLp190Susceptibility to Confer the Metabolic Shift Essential for pB-ALL. Cancer Research 2018, 78, 2669-2679, 10.1158/0008-5472.can-17-3262.
- Idoia García‐Ramírez; Sanil Bhatia; Guillermo Rodríguez‐Hernández; Inés González‐Herrero; Carolin Walter; Sara González De Tena‐Dávila; Salma Parvin; Oskar Haas; Wilhelm Woessmann; Martin Stanulla; et al. Lmo2 expression defines tumor cell identity during T‐cell leukemogenesis. The EMBO Journal 2018, 37, e98783, 10.15252/embj.201798783.
- Vicente-Dueñas, C.; González-Herrero, I.; Sehgal, L.; García-Ramírez, I.; Rodríguez-Hernández, G.; Pintado, B.; Blanco, O.; Criado, F.J.G.; Cenador, M.B.G.; Green, M.R.; et al. Dnm1 links BCR-ABLp210 to epigenetic tumour stem cell priming in myeloid leukaemia. Leukaemia 2019, 33, 249–278.
- Carolina Vicente-Dueñas; Julia Hauer; César Cobaleda; Arndt Borkhardt; Isidro Sanchez-Garcia; Epigenetic Priming in Cancer Initiation.. Trends in Cancer 2018, 4, 408-417, 10.1016/j.trecan.2018.04.007.
- Adriana Sánchez-Danés; Cédric Blanpain; Deciphering the cells of origin of squamous cell carcinomas. Nature Reviews Cancer 2018, 18, 549-561, 10.1038/s41568-018-0024-5.
- Hee-Je Kim; Mixed-phenotype acute leukemia (MPAL) and beyond. Blood Research 2016, 51, 215-216, 10.5045/br.2016.51.4.215.
- Mohamed Abdelhaleem; Frequent but nonrandom expression of myeloid markers on de novo childhood acute lymphoblastic leukemia. Experimental and Molecular Pathology 2007, 83, 138-141, 10.1016/j.yexmp.2007.02.004.
- K Iijima; K Sugita; T Inukai; K Goi; T Tezuka; K Uno; H Sato; K Kagami; S Nakazawa; Expression of thrombopoietin receptor and its functional role in human B-precursor leukemia cells with 11q23 translocation or Philadelphia chromosome.. Leukemia 2000, 14, 1598-1605, 10.1038/sj.leu.2401893.
- M Pérez-Caro; N Gutierrez-Cianca; I González-Herrero; I López-Hernández; T Flores; A Orfao; Manuel Sanchez-Martin; Alfonso Gutierrez-Adan; Belen Pintado; Isidro Sanchez-Garcia; et al. Sustained leukaemic phenotype after inactivation of BCR-ABLp190 in mice. Oncogene 2006, 26, 1702-1713, 10.1038/sj.onc.1209968.
- Raghu Kalluri; Robert A. Weinberg; The basics of epithelial-mesenchymal transition. Journal of Clinical Investigation 2009, 119, 1420-1428, 10.1172/JCI39104C1.
- Justine J Cunningham; Thomas M Ulbright; Martin F Pera; Leendert H J Looijenga; Lessons from human teratomas to guide development of safe stem cell therapies. Nature Biotechnology 2012, 30, 849-857, 10.1038/nbt.2329.
- K Shinjo; A Takeshita; M Higuchi; K Ohnishi; R Ohno; Erythropoietin receptor expression on human bone marrow erythroid precursor cells by a newly-devised quantitative flow-cytometric assay.. British Journal of Haematology 1997, 96, 551-558, .
- Motonari Kondo; David C. Scherer; Toshihiro Miyamoto; Angela G. King; Koichi Akashi; Kazuo Sugamura; Irving L. Weissman; Cell-fate conversion of lymphoid-committed progenitors by instructive actions of cytokines. Nature 2000, 407, 383-386, 10.1038/35030112.
- Noushine Mossadegh-Keller; Sandrine Sarrazin; Prashanth K. Kandalla; Leon Espinosa; E. Richard Stanley; Stephen L. Nutt; Jordan Moore; Michael H. Sieweke; M-CSF instructs myeloid lineage fate in single haematopoietic stem cells. Nature 2013, 497, 239-243, 10.1038/nature12026.
- Ciaran James Mooney; Alan Cunningham; Panagiotis Tsapogas; Kai-Michael Toellner; Geoffrey Brown; Selective Expression of Flt3 within the Mouse Hematopoietic Stem Cell Compartment. International Journal of Molecular Sciences 2017, 18, 1037, 10.3390/ijms18051037.
- Liu, F.; Poursine-Laurent, J.; Link, D.C.; Expression of the G-CSF receptor on hematopoietic progenitor cells is not required for their mobilisation by G-CSF. Blood 2000, 95, 3025–3031, .
- Gina Balciunaite; Rod Ceredig; Steffen Massa; Antonius G. Rolink; Rhodri Ceredig; A B220+ CD117+ CD19± hematopoietic progenitor with potent lymphoid and myeloid developmental potential. European Journal of Immunology 2005, 35, 2019-2030, 10.1002/eji.200526318.
- Llucia Alberti-Servera; Lilly Muenchow1; Panagiotis Tsapogas1; Giuseppina Capoferri1; Katja Eschbach2; Christian Beisel2; Rhodri Ceredig3; 5 And Robert Ivanek4; Antonius Rolink1; Single‐cell RNA sequencing reveals developmental heterogeneity among early lymphoid progenitors. The EMBO Journal 2017, 36, 3619-3633, 10.15252/embj.201797105.
- Ceredig, R.; Rolink, A.G.; Brown, G. Models of hematopoiesis: Seeing the wood for the trees. Nat. Rev. Immunol. 2009, 9, 93–300.
- Geoffrey Brown; Panagiotis Tsapogas; Rhodri Ceredig; The changing face of hematopoiesis: a spectrum of options is available to stem cells. Immunology and Cell Biology 2018, 96, 898-911, 10.1111/imcb.12055.
- Geoffrey Brown; Rhodri Ceredig; Modeling the Hematopoietic Landscape. Frontiers in Cell and Developmental Biology 2019, 7, 104, 10.3389/fcell.2019.00104.
- Bethan Psaila; Adam J. Mead; Single-cell approaches reveal novel cellular pathways for megakaryocyte and erythroid differentiation. Blood 2019, 133, 1427-1435, 10.1182/blood-2018-11-835371.
- Sonia Nestorowa; Fiona K. Hamey; Blanca Pijuan Sala; Evangelia Diamanti; Mairi Shepherd; Elisa Laurenti; Nicola K. Wilson; David G. Kent; Berthold Göttgens; A single-cell resolution map of mouse hematopoietic stem and progenitor cell differentiation. Blood 2016, 128, e20-e31, 10.1182/blood-2016-05-716480.
- Gina Balciunaite; Rhodri Ceredig; Antonius G. Rolink; The earliest subpopulation of mouse thymocytes contains potent T, significant macrophage, and natural killer cell but no B-lymphocyte potential. Blood 2005, 105, 1930-1936, 10.1182/blood-2004-08-3087.
- Andre Olsson; Meenakshi Venkatasubramanian; Viren K. Chaudhri; Bruce J. Aronow; Nathan Salomonis; Harinder Singh; H. Leighton Grimes; Single-cell analysis of mixed-lineage states leading to a binary cell fate choice. Nature 2016, 537, 698-702, 10.1038/nature19348.
- Amit Grover; Elena Mancini; Susan Moore; Adam J. Mead; Deborah Atkinson; Kasper Dindler Rasmussen; Donal O’Carroll; Sten Eirik W. Jacobsen; Claus Nerlov; Erythropoietin guides multipotent hematopoietic progenitor cells toward an erythroid fate. The Journal of Experimental Medicine 2014, 211, 181-188, 10.1084/jem.20131189.
- Michael A. Rieger; Philipp S. Hoppe; Benjamin M. Smejkal; Andrea C. Eitelhuber; Timm Schroeder; Hematopoietic Cytokines Can Instruct Lineage Choice. Science 2009, 325, 217-218, 10.1126/science.1171461.
- D. Metcalf; A. W. Burgess; Clonal analysis of progenitor cell commitment to granulocyte or macrophage production. Journal of Cellular Physiology 1982, 111, 275-283, 10.1002/jcp.1041110308.
- Panagiotis Tsapogas; Lee Kim Swee; Anja Nusser; Natko Nuber; Matthias Kreuzaler; Giuseppina Capoferri; Hannie Rolink; Rhodri Ceredig; Antonius Rolink; In vivo evidence for an instructive role of fms-like tyrosine kinase-3 (FLT3) ligand in hematopoietic development. Haematologica 2014, 99, 638-646, 10.3324/haematol.2013.089482.
- Griffin, J.D.; Lowenberg, B.; Clonogenic cells in acute myeloblastic leukaemia. Blood 1986, 68, 1185–1195, .
- Rashim Pal Singh; Tatyana Grinenko; Beáta Ramasz; Kristin Franke; Mathias Lesche; Andreas Dahl; Max Gassmann; Triantafyllos Chavakis; Ian Henry; Ben Wielockx; et al. Hematopoietic Stem Cells but Not Multipotent Progenitors Drive Erythropoiesis during Chronic Erythroid Stress in EPO Transgenic Mice. Stem Cell Reports 2018, 10, 1908-1919, 10.1016/j.stemcr.2018.04.012.
- Geoffrey Brown; Isidro Sánchez-García; Is lineage decision-making restricted during tumoral reprograming of haematopoietic stem cells?. Oncotarget 2015, 6, 43326-43341, 10.18632/oncotarget.6145.