Topic review

3D Bone Bioprinting

Subjects: Others View times: 32
Submitted by: Ilaria Roato

Definition

Every year, approximately a couple of million bone grafts are performed worldwide to treat bone lesions, of which about 1 million only in Europe, thus bone regeneration is necessary to replace the damaged tissue, while the improvement of bone healing, both qualitatively and quantitatively, is mandatory. Bone tissue is constituted by cells with functions carefully coordinated, and a complex cross-talk between bone forming and inflammatory cells is known to guide successful regeneration, thus repairing bone is not an easy task. Autografts are still considered the gold standard for repairing bone defects, although they are not without significant drawbacks, such as donor site availability and possible morbidity. To overcome the pitfalls of grafts, researchers relied on bone tissue engineering (BTE) and 3D bioprinting techniques to produce cell-laden scaffolds, in which bone biological components are assembled to form a 3D environment. Several techniques of bone bioprinting have been developed: inkjet, extrusion and light-based 3D printers, which use different bioinks, i.e., the printing materials.

1. 3D Bioprinting

3D bioprinting is a cutting-edge technology with a broad utility in bone tissue engineering (BTE) and regenerative medicine (RM) [1][2]. It is used to build constructs starting from a single cell type using layer-by-layer deposition of specific bioinks, which are essentially the biological components needed for the scaffold. Therefore, 3D bioprinting allows to develop highly reproducible, spatially controlled structures made of different materials, growth factors and cells, such as synthetic bone substitutes.The great advantage of 3D bioprinting relies in the potentiality to spatially distribute the cells within the solid or semi-solid biomaterials, thus optimizing tissue regeneration [3]. The development of 3D-bioprinted bone tissues is of great relevance and impact on clinical practice, because it also allows the reconstruction of bone defects with complex shape, just by translating computed tomography (CT) or microCT data of defects to printable image of them, leading to patient-specific implants [4][5][6]. The ideal scaffold should resemble a 3D structure and composition of human bone, it has a resorption rate that gives time to the bone from the recipient site to replace it, it provides nourishment of the graft cells and allows vascularization, which is essential for the graft success and a higher bone healing ability compared to non-osteoinductive ceramics [7]. Moreover, 3D bioprinting allows the production of constructs with different geometrics, porosity, and sizes, which are features relevant to obtain more osteoinductive scaffolds. Generally, osteoblasts or progenitor cells need a proper stimulation by bone morphogenetic proteins (BMPs) [8] for osteogenic differentiation, but some biomaterials, such as calcium phosphate (CaP) ceramics can induce an intrinsic osteoinduction, where mesenchymal stem cells (MSCs) differentiate into osteoblasts, even without exogeneous BMPs, avoiding the adverse effects of BMPs treatment [9][10][11][12]

Bioinks

Bioinks are the key components of bioprinting technology; they include printable organic and inorganic materials, biological factors and other components that enhance cell growth, differentiation and preserve shape fidelity during free-form deposition as extruded filaments [13][14][15][16]. Bioinks useful to obtain effective bone substitutes require properties including biocompatibility, biomimicry, biodegradability, bioprintability and mechanical integrity [17][18]. Thus, the design of the appropriate bioink is probably the main challenge of bioprinting [19][20]. Importantly, bioprinting of bone requires the use of bioinks capable of transitioning from a liquid state to a gel structure, without compromising cell viability and bioactivity [6]. Since bone is exposed to different and not uniform mechanical stress, and to various nutritional and vascular needs, bioinks must possess physical properties providing aid for cell differentiation by ensuring a favorable 3D microenvironment [21]. Starting from the introduction of cross-linkable bioinks, such as methacrylated gelatin and hyaluronan, more and more new materials are being engaged to make optimized bioinks. Another approach relies on the use of composite materials, which combine the advantages of each bioink, improving their mechanical strength, printability, biocompatibility, and gelation characteristics [22][23][24]. Macrostructural and geometry properties of material have a deep impact on the effectiveness of a scaffold, because porous materials, characterized by numerous pores of variable size and connectivity, are suitable for the passage of oxygen, nutrients, and cellular wastes. Notably, the porosity of the cell-laden scaffold is known to affect tissue formation and concomitant angiogenesis, which are two critical aspects for BTE [25][26][27]. Tarafder et al. [28] showed in a rat model that the control of the pore size resulted in an increased compressive strength, cell density, biocompatibility, and osteogenesis [28]. Various scaffolds based on different bioactive nanomaterials have been tested for their capabilities to induce new bone formation. For instance, hydroxyapatite (HA) nanoparticles showed a favorable osteoinductive activity on MSCs. In particular, HA nanostructured with concave macroporosity, derived from CaP crystals, accelerate osteoinduction, since they are chemically and structurally similar to those of the natural bone tissue [29]. Other nanoparticles made by different components, such as molybdenum-doped bioactive glass [30], magnetic iron oxide [31], strontium containing bioactive glass [32], and gold [33] showed osteoinductive abilities. Depending on the final aim, the cells can be deposited onto the scaffold biomaterial during the printing process, generating the scaffold-based bioinks [19] or, alternatively, they can be directly printed embedded in the biomaterial, implementing the scaffold-free bioinks [34][35][36].

2. 3D Bioprinting Applications to Treat Bone Defects

Besides BTE, 3D bioprinting is strongly relevant in the field of cancer research, where 2D tumor models do not reconstitute the complexity of the dynamic tumor microenvironment [37]. Conversely, 3D-bioprinted models allow for reproduction of cell–cell and cell–matrix interactions and have the advantage to integrate a vascular system to study tumor angiogenesis [38]. Hence, the tumor tissue should be placed within a bioprinted vascularized parenchyma to analyze how cancer cells grow and other carcinogenic events, i.e., intravasation and extravasation [39]. Important to note is that a 3D biomimetic bone matrix has been used to create a model of breast cancer bone metastases, with a bone like microenvironment that provides cross-talk among breast cancer cells, human bone marrow MSCs, and osteoblasts [40]. Zhu et al. [41] used a 3D printed nano-ink, made of hydroxyapatite nanoparticles suspended in hydrogel, to simulate a bone-specific environment to study breast cancer bone invasion.

The potential applications of BTE in orthopedics are enormous since can solve both bone and cartilage problems [42]. A comprehensive review analyzing the application of BTE for orthopedic trauma according to the different anatomical sites, showed its usefulness to treat bone trauma in a patient-specific manner [43]. Alba et al. [44]developed a new method to engineer periosteum tissue by printing periosteal derived cells (PDCs) mixed with alginate on collagen scaffolds. The presence of collagen contributed to maintain the structural integrity and osteogenic differentiation of PDCs, which was demonstrated by osteocalcin and alkaline phosphatase gene expression.

A multi-component bioink, constituted by wood-based nano-cellulose and bioactive glass to strengthen gelatin-alginate bioinks, was tested and resulted effective in sustaining bone cell viability, proliferation, and osteodifferentiation [44].

Cartilage tissue defects are difficult to repair due to cartilage poor self-repairing capacity, thus the potential to re-create functional articular cartilage by 3D bioprinting is contemporary tempting and challenging. Cartilage must sustain heavy loads, therefore a hybrid scaffold, constituted by PCL with rabbit chondrocytes and fibrin collagen hydrogel, was fabricated to enhance mechanical and biological properties for load-bearing cartilage. The authors showed that this hybrid construct formed cartilage-like tissues both in vitro and in vivo, as evidenced by the deposition of type II collagen and glycosaminoglycans [45]. Daly et al. [46] used an MSC-laden bioink (arginine-glycine-aspartic acid (RGD)-modified alginate hydrogels) co-deposited with PCL fibers, which showed s 350-fold increase in compressive modulus of bioink/PCL templates. The constructs had the potential to be implanted as vertebral bodies in load bearing locations.

O’Connell et al. [47][48] developed a device named “Biopen”, which is basically an EBB bioprinter for in vivo application directly during the surgery. This Biopen was utilized to repair chondral defects in a large animal ovine model [49]. Repairing an osteochondral defect remains the most challenging part of engineering implants for full thickness osteochondral lesions, which can be repaired through a modular tissue assembly strategy, according to Schon et al. [50].

Furthermore, 3D-printed tissue models may be used to test the efficacy and toxicity of new drug candidates mimicking the native tissue, thus fostering translation of new therapeutic molecules into clinics [51][52]. Compared to other types of 3D in vitro systems [53], 3D bioprinting has numerous advantages such as the controllability, the high-throughput capability, and the generation of drug-delivery vehicles precisely [54]. Indeed, the DVDOD technology delivers droplets to a specific location in a volumetric manner with a high-throughput capability. This technique has been tested to bioprint pre-osteoblast cells with alginate hydrogel into bone damaged tissue, in a minimally invasive manner, showing the formation of functional tissue [55].

Recently, a 3D bioprinted pseudo-bone drug delivery scaffold for simvastatin was generated to promote bone healing. This scaffold displayed matrix strength, matrix resilience, and porous morphology of healthy human bone [56].

In another work, 3D printed PCL/hydrogel composite scaffolds, loaded with bioactive small molecules (i.e., resveratrol and strontium ranelate) able to target bone cells, have been generated and studied to treat craniomaxillofacial defects. The authors implanted the 3D printed scaffolds, with and without small molecules into a rat model with a critical-sized mandibular bone defect, demonstrating that the bone scaffolds, carried with small molecules, showed enhanced angiogenesis, inhibition of osteoclast activities, and stimulation of MSC osteogenic differentiation with consequent in vivo mandibular bone formation eight weeks after implantation [57]. In Table 2, we present some works potentially relevant for their clinical implications, where 3D bioprinting resulted as useful in repairing bone defects.

Table 2. Applications of 3D Bioprinting on bone defects.

Cell Types, Molecules Bioink Bioprinting Modality Application
Bone marrow MSCs, osteoblast GelMA + nanocrystalline HA [40] LBB (Stereolithography) Breast cancer bone metastases
Osteoblast, breast cancer cells PEG hydrogel + nanocrystalline HA [58]
Hydrogel resins (PEG, PEG-diacryilate) [41]
LBB
(Stereolithography)
Breast cancer bone metastases
Without cells (PLA) and acrylonitrile butadiene styrene (ABS) [57] EBB with Fused deposition model (FDM) Radius fracture repair
Periosteal derived cells Alginate hydrogel + collagen I, II [44][59] EBB by piston-driven system Periosteum Tissue Engineering
MSCs RGD alginate hydrogels [46] EBB by multiple-head 3D printing system To engineer endochondral bone
ASCs HA-GelMA [49][50] EBB by Biopen Regeneration of chondral lesions
Meniscal fibrochondrocytes (MFCs) meniscus extracellular matrix (MECM)-based hydrogel [60] 3D printing fused deposition modeling Meniscus regeneration
IPS cells, 143B human osteosarcoma cells, preosteoblasts MC3T3 Alginate hydrogel [55] Direct- volumetric Drop-on-demand (DVDOD) technology Microtissue fabrication and drug delivery
Simvastatin copolymeric blend of polymers: polypropylene fumarate (PPF), PEG-PCL-PEG, and pluronic PF 127 [56] LBB Drug delivery
Resveratrol and strontium ranelate PCL/hydrogel [57] EBB Cranio-maxillofacial regeneration

3. Conclusions and Remarks

Even though clinical application of bioprinting technology is still in its infancy, the production of entire and functional organs characterized by relevant dimensions is an attractive challenge in TE. As portrayed before, to get closer to this ambitious goal, several aspects should be considered, such as a functional and hierarchical organized vascular network integrated in the system and the incorporation of the various cell types involved in the organ biology [61][62]. Bone may become paradigmatic in this process, as it seems to be more ahead than other tissues in its way toward clinical application. Significant progress has been made in 3D bioprinting for BTE, combining biomaterials, cells, and factor to obtain engineered bone tissue grafts, able to promote bone regeneration. For instance, bioprinted bone was successfully implanted in pre-clinical models [63] and 3D-printed plastic, ceramic, or metallic implants for bone tissue replacement [62] have been successfully transplanted into humans. Finally, a recent work demonstrated a unique case of transplantation of a 3D-printed bio-resorbable airway splint into an infant [64].

The exponential interest in these technologies is leading multidisciplinary teams to develop new bioinks [19] and post-printing procedures. Indeed, thanks to new self-absorbing polymers and the correct incorporation of specific molecules, mechanical, structural, and biocompatibility properties of these materials will be increased to recreate a correct milieu.

The other great technological challenge will be played in the management of post-printing procedures. In fact, more and more companies are developing different types of bioreactor, both in the field of millufluidics and microfluidics. Correct metabolic management and mechanical stimuli of BTE will therefore be possible.

In conclusion, considering the fast evolution of technology, in the next decade it is plausible to expect that volumetric composite tissues with native tissue-like properties will become printable. Indeed, the development of advanced high-resolution bioprinters with multiple modalities and print-heads (such as the newly created ITOP [65]), will lay the foundation for creating complex heterocellular and vascularized tissues. In this regard, the recent development of 4D bioprinting technology [66] could play a key role, since the integration of the concept of time with the 3D bioprinting technology will permit the development of tissues with high levels of complexity and size [67]. This aspect is particularly relevant since natural tissue regeneration is subjected to dynamic modifications of macro-/micro-structures and composition due to different intrinsic and external stimuli. Thus, a sort of maturation and functionalization of the 3D-bioprinted tissue with time is necessary and can be achieved by 4D bioprinting technology [68].

The technological complexity in these fields will make the need for laboratories with extremely multidisciplinary skills increasingly evident. Moreover, standardized regulatory protocols will need to be established, above all considering the even more increasing necessity to translate into clinical practice the use of these TE products.

The entry is from 10.3390/ijms21197012

References

  1. Jakab, K.; Norotte, C.; Marga, F.; Murphy, K.; Vunjak-Novakovic, G.; Forgacs, G. Tissue engineering by self-assembly and bio-printing of living cells. Biofabrication 2010, 2, 22001.
  2. Moroni, L.; de Wijn, J.R.; van Blitterswijk, C.A. 3D fiber-deposited scaffolds for tissue engineering: Influence of pores geometry and architecture on dynamic mechanical properties. Biomaterials 2006, 27, 974–985.
  3. Gao, G.; Cui, X. Three-dimensional bioprinting in tissue engineering and regenerative medicine. Biotechnol. Lett. 2016, 38, 203–211.
  4. Li, L.; Yu, F.; Shi, J.; Shen, S.; Teng, H.; Yang, J.; Wang, X.; Jiang, Q. In situ repair of bone and cartilage defects using 3D scanning and 3D printing. Sci. Rep. 2017, 7, 9416.
  5. Costa, J.B.; Silva-Correia, J.; Ribeiro, V.P.; da Silva Morais, A.; Oliveira, J.M.; Reis, R.L. Engineering patient-specific bioprinted constructs for treatment of degenerated intervertebral disc. Mater. Today Commun. 2019, 19, 506–512.
  6. Datta, P.; Ozbolat, V.; Ayan, B.; Dhawan, A.; Ozbolat, I.T. Bone tissue bioprinting for craniofacial reconstruction. Biotechnol. Bioeng. 2017, 114, 2424–2431.
  7. Habibovic, P.; Yuan, H.; van den Doel, M.; Sees, T.M.; van Blitterswijk, C.A.; de Groot, K. Relevance of osteoinductive biomaterials in critical-sized orthotopic defect. J. Orthop. Res. Off. Publ. Orthop. Res. Soc. 2006, 24, 867–876.
  8. Barradas, A.M.C.; Yuan, H.; van Blitterswijk, C.A.; Habibovic, P. Osteoinductive biomaterials: Current knowledge of properties, experimental models and biological mechanisms. Eur. Cell. Mater. 2011, 21, 407–429.
  9. Wong, D.A.; Kumar, A.; Jatana, S.; Ghiselli, G.; Wong, K. Neurologic impairment from ectopic bone in the lumbar canal: A potential complication of off-label PLIF/TLIF use of bone morphogenetic protein-2 (BMP-2). Spine J. 2008, 8, 1011–1018.
  10. Kaneko, H.; Arakawa, T.; Mano, H.; Kaneda, T.; Ogasawara, A.; Nakagawa, M.; Toyama, Y.; Yabe, Y.; Kumegawa, M.; Hakeda, Y. Direct stimulation of osteoclastic bone resorption by bone morphogenetic protein (BMP)-2 and expression of BMP receptors in mature osteoclasts. Bone 2000, 27, 479–486.
  11. Smucker, J.D.; Rhee, J.M.; Singh, K.; Yoon, S.T.; Heller, J.G. Increased swelling complications associated with off-label usage of rhBMP-2 in the anterior cervical spine. Spine 2006, 31, 2813–2819.
  12. Zara, J.N.; Siu, R.K.; Zhang, X.; Shen, J.; Ngo, R.; Lee, M.; Li, W.; Chiang, M.; Chung, J.; Kwak, J.; et al. High doses of bone morphogenetic protein 2 induce structurally abnormal bone and inflammation in vivo. Tissue Eng. Part A 2011, 17, 1389–1399.
  13. Datta, P.; Barui, A.; Wu, Y.; Ozbolat, V.; Moncal, K.K.; Ozbolat, I.T. Essential steps in bioprinting: From pre- to post-bioprinting. Biotechnol. Adv. 2018, 36, 1481–1504.
  14. Chimene, D.; Lennox, K.K.; Kaunas, R.R.; Gaharwar, A.K. Advanced Bioinks for 3D Printing: A Materials Science Perspective. Ann. Biomed. Eng. 2016, 44, 2090–2102.
  15. Stanton, M.M.; Samitier, J.; Sánchez, S. Bioprinting of 3D hydrogels. Lab Chip 2015, 15, 3111–3115.
  16. Hölzl, K.; Lin, S.; Tytgat, L.; Van Vlierberghe, S.; Gu, L.; Ovsianikov, A. Bioink properties before, during and after 3D bioprinting. Biofabrication 2016, 8, 32002.
  17. Mandrycky, C.; Wang, Z.; Kim, K.; Kim, D.-H. 3D bioprinting for engineering complex tissues. Biotechnol. Adv. 2016, 34, 422–434.
  18. Adepu, S.; Dhiman, N.; Laha, A.; Sharma, C.S.; Ramakrishna, S.; Khandelwal, M. Three-dimensional bioprinting for bone tissue regeneration. Curr. Opin. Biomed. Eng. 2017, 2, 22–28.
  19. Hospodiuk, M.; Dey, M.; Sosnoski, D.; Ozbolat, I.T. The bioink: A comprehensive review on bioprintable materials. Biotechnol. Adv. 2017, 35, 217–239.
  20. Zhang, B.; Gao, L.; Ma, L.; Luo, Y.; Yang, H.; Cui, Z. 3D Bioprinting: A Novel Avenue for Manufacturing Tissues and Organs. Engineering 2019, 5, 777–794.
  21. Chawla, S.; Midha, S.; Sharma, A.; Ghosh, S. Silk-Based Bioinks for 3D Bioprinting. Adv. Healthc. Mater. 2018, 7, 1701204.
  22. Skardal, A.; Zhang, J.; McCoard, L.; Xu, X.; Oottamasathien, S.; Prestwich, G.D. Photocrosslinkable hyaluronan-gelatin hydrogels for two-step bioprinting. Tissue Eng. Part A 2010, 16, 2675–2685.
  23. Thakur, P.T.; Cabrera, D.D.; DeCarolis, N.; Boni, A.A. Innovation and Commercialization Strategies for Three-Dimensional- Bioprinting Technology: A Lean Business Model Perspective. J. Commer. Biotechnol. 2018, 24, 78–87.
  24. Lieben, L. Regenerative medicine: The future of 3D printing of human tissues is taking shape. Nat. Rev. Rheumatol. 2016, 12, 191.
  25. Karageorgiou, V.; Kaplan, D. Porosity of 3D biomaterial scaffolds and osteogenesis. Biomaterials 2005, 26, 5474–5491.
  26. Hollister, S.J. Porous scaffold design for tissue engineering. Nat. Mater. 2005, 4, 518–524.
  27. Fedorovich, N.E.; Kuipers, E.; Gawlitta, D.; Dhert, W.J.A.; Alblas, J. Scaffold porosity and oxygenation of printed hydrogel constructs affect functionality of embedded osteogenic progenitors. Tissue Eng. Part A 2011, 17, 2473–2486.
  28. Tarafder, S.; Balla, V.K.; Davies, N.M.; Bandyopadhyay, A.; Bose, S. Microwave-sintered 3D printed tricalcium phosphate scaffolds for bone tissue engineering. J. Tissue Eng. Regen. Med. 2013, 7, 631–641.
  29. Liang, H.; Xu, X.; Feng, X.; Ma, L.; Deng, X.; Wu, S.; Liu, X.; Yang, C. Gold nanoparticles-loaded hydroxyapatite composites guide osteogenic differentiation of human mesenchymal stem cells through Wnt/β-catenin signaling pathway. Int. J. Nanomedicine 2019, 14, 6151–6163.
  30. Guo, Y.; Xue, Y.; Niu, W.; Chen, M.; Wang, M.; Ma, P.; Lei, B. Monodispersed Bioactive Glass Nanoparticles Enhance the Osteogenic Differentiation of Adipose-Derived Stem Cells through Activating TGF-Beta/Smad3 Signaling Pathway. Part. Part. Syst. Charact. 2018, 35.
  31. Wang, Q.; Chen, B.; Ma, F.; Lin, S.; Cao, M.; Li, Y.; Gu, N. Magnetic iron oxide nanoparticles accelerate osteogenic differentiation of mesenchymal stem cells via modulation of long noncoding RNA INZEB2. Nano Res. 2017, 10, 626–642.
  32. Naruphontjirakul, P.; Tsigkou, O.; Li, S.; Porter, A.E.; Jones, J.R. Human mesenchymal stem cells differentiate into an osteogenic lineage in presence of strontium containing bioactive glass nanoparticles. Acta Biomater. 2019, 90, 373–392.
  33. Zhang, D.; Liu, D.; Zhang, J.; Fong, C.; Yang, M. Gold nanoparticles stimulate differentiation and mineralization of primary osteoblasts through the ERK/MAPK signaling pathway. Mater. Sci. Eng. C Mater. Biol. Appl. 2014, 42, 70–77.
  34. Norotte, C.; Marga, F.S.; Niklason, L.E.; Forgacs, G. Scaffold-free vascular tissue engineering using bioprinting. Biomaterials 2009, 30, 5910–5917.
  35. Yu, Y.; Moncal, K.K.; Li, J.; Peng, W.; Rivero, I.; Martin, J.A.; Ozbolat, I.T. Three-dimensional bioprinting using self-assembling scalable scaffold-free “tissue strands” as a new bioink. Sci. Rep. 2016, 6, 28714.
  36. Ozbolat, I.T. Scaffold-based or scaffold-free bioprinting: Competing or complementing approaches? J. Nanotechnol. Eng. Med. 2015, 6, 024701.
  37. Perkins, J.D. Are we reporting the same thing? Liver Transplant. Off. Publ. Am. Assoc. Study Liver Dis. Int. Liver Transplant. Soc. 2007, 13, 465–466.
  38. Qiao, H.; Tang, T. Engineering 3D approaches to model the dynamic microenvironments of cancer bone metastasis. Bone Res. 2018, 6, 3.
  39. Ozbolat, I.T.; Peng, W.; Ozbolat, V. Application areas of 3D bioprinting. Drug Discov. Today 2016, 21, 1257–1271.
  40. Zhou, X.; Zhu, W.; Nowicki, M.; Miao, S.; Cui, H.; Holmes, B.; Glazer, R.I.; Zhang, L.G. 3D Bioprinting a Cell-Laden Bone Matrix for Breast Cancer Metastasis Study. ACS Appl. Mater. Interfaces 2016, 8, 30017–30026.
  41. Zhu, W.; Holmes, B.; Glazer, R.I.; Zhang, L.G. 3D printed nanocomposite matrix for the study of breast cancer bone metastasis. Nanomedicine 2016, 12, 69–79.
  42. Dhawan, A.; Kennedy, P.M.; Rizk, E.B.; Ozbolat, I.T. Three-dimensional Bioprinting for Bone and Cartilage Restoration in Orthopaedic Surgery. J. Am. Acad. Orthop. Surg. 2019, 27, e215–e226.
  43. Lal, H.; Patralekh, M.K. 3D printing and its applications in orthopaedic trauma: A technological marvel. J. Clin. Orthop. Trauma 2018, 9, 260–268.
  44. Alba, B.; Swami, P.; Tanna, N.; Grande, D. A Novel Technique for Tissue Engineering Periosteum Using Three-Dimensional Bioprinting. Plast. Reconstr. Surg. Glob. Open 2018, 6, 98.
  45. Xu, T.; Binder, K.W.; Albanna, M.Z.; Dice, D.; Zhao, W.; Yoo, J.J.; Atala, A. Hybrid printing of mechanically and biologically improved constructs for cartilage tissue engineering applications. Biofabrication 2013, 5, 15001.
  46. Daly, A.C.; Cunniffe, G.M.; Sathy, B.N.; Jeon, O.; Alsberg, E.; Kelly, D.J. 3D Bioprinting of Developmentally Inspired Templates for Whole Bone Organ Engineering. Adv. Healthc. Mater. 2016, 5, 2353–2362.
  47. Duchi, S.; Onofrillo, C.; O’Connell, C.D.; Blanchard, R.; Augustine, C.; Quigley, A.F.; Kapsa, R.M.I.; Pivonka, P.; Wallace, G.; Di Bella, C.; et al. Handheld Co-Axial Bioprinting: Application to in situ surgical cartilage repair. Sci. Rep. 2017, 7, 5837.
  48. O’Connell, C.D.; Di Bella, C.; Thompson, F.; Augustine, C.; Beirne, S.; Cornock, R.; Richards, C.J.; Chung, J.; Gambhir, S.; Yue, Z.; et al. Development of the Biopen: A handheld device for surgical printing of adipose stem cells at a chondral wound site. Biofabrication 2016, 8, 15019.
  49. Di Bella, C.; Duchi, S.; O’Connell, C.D.; Blanchard, R.; Augustine, C.; Yue, Z.; Thompson, F.; Richards, C.; Beirne, S.; Onofrillo, C.; et al. In situ handheld three-dimensional bioprinting for cartilage regeneration. J. Tissue Eng. Regen. Med. 2018, 12, 611–621.
  50. Schon, B.S.; Hooper, G.J.; Woodfield, T.B.F. Modular Tissue Assembly Strategies for Biofabrication of Engineered Cartilage. Ann. Biomed. Eng. 2017, 45, 100–114.
  51. Ozbolat, I.T.; Hospodiuk, M. Current advances and future perspectives in extrusion-based bioprinting. Biomaterials 2016, 76, 321–343.
  52. Snyder, J.E.; Hamid, Q.; Wang, C.; Chang, R.; Emami, K.; Wu, H.; Sun, W. Bioprinting cell-laden matrigel for radioprotection study of liver by pro-drug conversion in a dual-tissue microfluidic chip. Biofabrication 2011, 3, 34112.
  53. Xu, F.; Wu, J.; Wang, S.; Durmus, N.G.; Gurkan, U.A.; Demirci, U. Microengineering methods for cell-based microarrays and high-throughput drug-screening applications. Biofabrication 2011, 3, 34101.
  54. Peng, W.; Datta, P.; Ayan, B.; Ozbolat, V.; Sosnoski, D.; Ozbolat, I.T. 3D bioprinting for drug discovery and development in pharmaceutics. Acta Biomater. 2017, 57, 26–46.
  55. Grottkau, B.E.; Hui, Z.; Pang, Y. A Novel 3D Bioprinter Using Direct-Volumetric Drop-On-Demand Technology for Fabricating Micro-Tissues and Drug-Delivery. Int. J. Mol. Sci. 2020, 21, 3482.
  56. Kondiah, P.J.; Kondiah, P.P.D.; Choonara, Y.E.; Marimuthu, T.; Pillay, V. A 3D Bioprinted Pseudo-Bone Drug Delivery Scaffold for Bone Tissue Engineering. Pharmaceutics 2020, 12, 166.
  57. Zhang, W.; Shi, W.; Wu, S.; Kuss, M.; Jiang, X.; Untrauer, J.B.; Reid, S.P.; Duan, B. 3D printed composite scaffolds with dual small molecule delivery for mandibular bone regeneration. Biofabrication 2020, 12, 35020.
  58. Zhu, W.; Castro, N.J.; Cui, H.; Zhou, X.; Boualam, B.; McGrane, R.; Glazer, R.I.; Zhang, L.G. A 3D printed nano bone matrix for characterization of breast cancer cell and osteoblast interactions. Nanotechnology 2016, 27, 315103.
  59. Jia, J.; Richards, D.J.; Pollard, S.; Tan, Y.; Rodriguez, J.; Visconti, R.P.; Trusk, T.C.; Yost, M.J.; Yao, H.; Markwald, R.R.; et al. Engineering alginate as bioink for bioprinting. Acta Biomater. 2014, 10, 4323–4331.
  60. Chen, M.; Feng, Z.; Guo, W.; Yang, D.; Gao, S.; Li, Y.; Shen, S.; Yuan, Z.; Huang, B.; Zhang, Y.; et al. PCL-MECM-Based Hydrogel Hybrid Scaffolds and Meniscal Fibrochondrocytes Promote Whole Meniscus Regeneration in a Rabbit Meniscectomy Model. ACS Appl. Mater. Interfaces 2019, 11, 41626–41639.
  61. Dolati, F.; Yu, Y.; Zhang, Y.; De Jesus, A.M.; Sander, E.A.; Ozbolat, I.T. In vitro evaluation of carbon-nanotube-reinforced bioprintable vascular conduits. Nanotechnology 2014, 25, 145101.
  62. Zhang, Y.; Yu, Y.; Akkouch, A.; Dababneh, A.; Dolati, F.; Ozbolat, I.T. In Vitro Study of Directly Bioprinted Perfusable Vasculature Conduits. Biomater. Sci. 2015, 3, 134–143.
  63. Keriquel, V.; Guillemot, F.; Arnault, I.; Guillotin, B.; Miraux, S.; Amédée, J.; Fricain, J.-C.; Catros, S. In vivo bioprinting for computer- and robotic-assisted medical intervention: Preliminary study in mice. Biofabrication 2010, 2, 14101.
  64. Bose, S.; Vahabzadeh, S.; Bandyopadhyay, A. Bone tissue engineering using 3D printing. Mater. Today 2013, 16, 496–504.
  65. Kang, H.-W.; Lee, S.J.; Ko, I.K.; Kengla, C.; Yoo, J.J.; Atala, A. A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nat. Biotechnol. 2016, 34, 312–319.
  66. Li, Y.-C.; Zhang, Y.; Akpek, A.; Shin, S.; Khademhosseini, A. 4D bioprinting: The next-generation technology for biofabrication enabled by stimuli-responsive materials. Biofabrication 2016, 9, 12001.
  67. Leberfinger, A.N.; Dinda, S.; Wu, Y.; Koduru, S.V.; Ozbolat, V.; Ravnic, D.J.; Ozbolat, I.T. Bioprinting functional tissues. Acta Biomater. 2019, 95, 32–49.
  68. Wan, Z.; Zhang, P.; Liu, Y.; Lv, L.; Zhou, Y. Four-dimensional bioprinting: Current developments and applications in bone tissue engineering. Acta Biomater. 2020, 101, 26–42.

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Molecular Biology ; – Created: 09 Sep 2020; Latest updated: 27 Oct 2020