Recent advances of cellulase immobilization onto magnetic nanoparticles

Subjects: Biotechnology View times: 269
Submitted by: Kamyar Khoshnevisan


Cellulosic enzymes including cellulase, play an important role in biotechnological processes in the fields of food, cosmetics, detergents, pulp, paper and related industries. Low thermal and storage stability of cellulase, presence of impurities, enzyme leakage and reusability poses a great challenge in all these processes. These challenges can be overcome via enzyme immobilization methods. In recent years, cellulase immobilization onto nanomaterials has become the focus of researchers attention owing to surface features of these materials. However, the application of these nanomaterials is limited due to the efficacy of their recovery process. Application of magnetic nanoparticles (MNPs) has been suggested as a solution to this problem since they can be easily removed from the reaction mixture by applying an external magnet. Recently, MNPs have been extensively employed for enzyme immobilization owing to their low toxicity and various practical advantages. In the present review, recent advances in cellulase immobilization onto functionalized MNPs is summarized. Finally, we have discussed enhanced enzyme reusability, activity and stability as well as improved enzyme recovery. Enzyme immobilization techniques offer promising potential for industrial applications.

 Aim of this study


A summary of different methods used for MNPs functionalization for cellulase immobilization is presented in Figure 1. These functionalization methods are further described and thier advantagous features are thoroughly discussed. The current review is focused on recent findings on immobilized cellulases on MNPs supports with various functionalized groups and their advantagous.

 An overview of functionalized-MNP by different methods.


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