Table of Contents

    Topic review

    Tithonia Diversifolia

    Subjects: Plant Sciences
    View times: 134
    Submitted by: Hisashi Kato-Noguchi


    Tithonia diversifolia (Hemsl.) A. Gray (Asteraceae) is native to Mexico and Central America. The species is spreading quickly and has naturalized in more than 70 countries. It has often been recorded as a harmful invasive plant which disturbs native plant communities. Phytotoxic chemical interactions such as allelopathy between invasive plants and native plants have been reported to play an important role in the invasion. Evidence for allelopathy of T. diversifolia has accumulated in the literature over 30 years. Thus, the objective of this review was to discuss the possible involvement of allelopathy in the invasive potential of T. diversifolia. The extracts, root exudates and plant residues of T. diversifolia inhibited the germination and growth of other plant species. The soil-water and soil collected from T. diversifolia fields also shown growth inhibitory effects. The decomposition rate of T. diversifolia residues in soil was reported to be high. Phytotoxic substances such as sesquiterpene lactones were isolated and identified in the extracts of T. diversifolia. Some of phytotoxic substances in T. diversifolia may be released into the soil through the decomposition of the plant residues and the exudation from living tissues of T. diversifolia including its root exudates, and act as allelopathic substances. Those allelopathic substances can inhibit the germination and growth of neighboring plants, and may enhance the competitive ability of the plants and make the plants invasive.

    1. Introduction

    Tithonia diversifolia (Hemsl.) A. Gray (phylum: Spermatophyta, class: Dicotyledonae, order: Asterales, family: Asteraceae, genus: Tithonia) is known as Mexican sunflower, tree marigold, or Nitobe chrysanthemum. It grows rapidly, reaching 2–3 m in height with large alternate lobe leaves (up to 45 cm long). The monocarpic capitulums are 10–30 cm long and bear bright yellow flowers (5–15 cm in diameter). The plant often forms pure stands with high density (8–20 plants/m2) [1][2][3].

    T. diversifolia can be harvested year-round and all parts of the plants have been used by indigenous people as folk medicine for a wide range of diseases and aliments, through topical administration to treat abdominal pain, wounding, dermatosis, and muscular disorder; and through oral administration to treat infection, malaria, fever, hepatitis, and diabetes [3][4]. Thus, the plants have a broad spectrum of medicinal values.

    More than a hundred secondary metabolites in many classes have been isolated from various parts of T. diversifolia extracts, including sesquiterpenoids, diterpenoids, and flavonoids. Tagitinins A, C, and F were first isolated from T. diversifolia [5][6]. The effects of the extracts of T. diversifolia and those compounds isolated from T. diversifolia have been widely studied in human cell lines, microorganisms, and some animal models. These studies showed an extended spectrum of biological activities for the extracts and compounds, such as anti-inflammatory and analgesic activities; antiprotozoal activity, including antimalarial effects; and antiviral and anticancer activities. The compounds of T. diversifolia and their pharmacological activities have been discussed in the review articles [3][4][7]. Therefore, T. diversifolia is one of the important sources of pharmacologically active substances, and the study of these compounds may contribute to developing potential medicines for various treatments.

    T. diversifolia also works as green manure, increasing crop productivity, and acts as fodder for domestic animals because of its high mineral and nutrient values [8][9][10][11]. On the other hand, T. diversifolia aggressively expands its habitat into agricultural and non-agricultural areas, becoming a serious farmland weed and disturbing native plant communities as an invasive plant species [2][7][12]. The species has shown allelopathic potency on the germination and growth of several other plant species [13][14][15]. Allelopathy may play an important role in the invasion of T. diversifolia. The objective of this review was to discuss the possible involvement of allelopathy in the invasive potential of T. diversifolia. Thus, this review summarized the allolopathic properties and invasive traits of T. diversifolia and discussed the importance of allelopathy for its invasive characteristics.

    2. Invasive Traits of T. diversifolia

    T. diversifolia is native to Mexico and Central America, but it is spreading quickly and has naturalized in more than 70 countries. The species has often been recorded as a harmful invasive plant in tropical and subtropical regions, threatening to disrupt agricultural crop production and native plant communities [2][3]. The life history characteristics, such as the high reproduction and high growth rate, as well as phenotypic plasticity of the plants, are important for the naturalization of invasive plants into non-native ranges [16][17][18]. T. diversifolia reproduces asexually and sexually, producing a large number (80,000–160,000 seeds/m2/year) of small seeds [1][12]. The phenotypic plasticity and genetic diversity of T. diversifolia populations were recorded to be high [19][20].

    The interactions of the invasive plants with natural enemies, such as herbivores and pathogens, are also very critical for their naturalization. The high defense capacity from herbivores and pathogens contributes to the ability of invasive plants to naturalize in non-native ranges [21][22][23]. Sesquterpene lactones and flavonoids of T. diversifolia probably act as defensive agents against herbivores and pathogens [3][4][7][24]. Insecticidal properties of the extracts and compounds of T. diversifolia have also been reported [10][24][25][26][27].

    In addition, the interactions of the invasive plants with native plants are crucial. In fact, some invasive plants contain many phytotoxic or allelopathic substances [28][29][30]. Centaures maculosa Lam. is invasive and releases an allelopathic substance, catechin, which is toxic and helps its invasion into bunchgrass fields [28]. Several other observations also suggest that some invasive plant species are allelopathic, and that their allelopathic substances are more toxic against other plant species in the invasive areas than those in native areas of the invasive plants [21][30][31]. Therefore, allelopathy is probably one of the important factors for invasive plants to naturalize and establish their habitats in non-native ranges [30][31]. As describe previously, T. diversifolia is allelopathic, and this allelopathic property may help the invasion of this species into non-native ranges.

    Many of the phytotoxic substances from the invasive plants have been reported to have multiple effects, such as antiherbivore, antifungal, antimicrobial, and allelopathic activities. The functions of these phytotoxic substances were considered to provide the plants with advantages in terms of increasing their populations in the new environments [18][30][31][32]. More than a hundred secondary metabolites have been isolated from various extracts of T. diversifolia, including sesquiterpenoids, diterpenoids, and flavonoids, while these compounds were also reported to possess wide ranges of biological activities [3][4][7][24]. Therefore, these compounds may enhance the competitive ability of T. diversifolia and make the plant invasive. The novel weapon hypothesis states that some invasive plant species gain a competitive advantage through the release of some compounds that are unique to the invading plant communities [30][31]. It is also possible that some of the compounds released from T. diversifolia were unique to the invaded plant communities.

    3. Conclusions

    T. diversifolia works as green manure, increasing crop productivity, and acts as fodder for domestic animals because of its high mineral and nutrient values [8][9][10][11]. However, the species has often been recorded as a harmful invasive plant that disturbs native plant communities [2][7][12]. The evidence summarized in this paper indicates that T. diversifolia is allelopathic and contains several phytotoxic substances, such as sesquiterpene lactones. The evidence also suggests that some of the phytotoxic substances in T. diversifolia are probably released into the soil through the decomposition of the plant residues and the exudation from living plant tissues of T. diversifolia, which act as allelopathic substances. The allelopathic substances can inhibit the germination and growth of neighboring plants [33][29][30][31]. Therefore, the allelopathic substances released from T. diversifolia may provide the plants with a competitive advantage against native plants, and may contribute to the plants establishing their habitats as invasive plant species. Allelopathy of T. diversifolia may be involved in the invasive potential of T. diversifolia.

    The entry is from 10.3390/plants9060766


    1. Muoghalu, J.I. Growth, reproduction and resource allocation of Tithonia diversifolia and Tithonia rotundifolia. Weed Res. 2008, 48, 157–162.
    2. Obiakara, M.C.; Fourcade, Y. Climatic niche and potential distribution of Tithonia diversifolia (Hemsl.) A. Gray in Africa. PLoS ONE 2018, 13, e0202421.
    3. Tagne, A.M.; Marino, F.; Cosentino, M. Tithonia diversifolia (Hemsl.) A. Gray as a medicinal plant: A comprehensive review of its ethnopharmacology, phytochemistry, pharmacotoxicology and clinical relevance. J. Ethnopharmacol. 2018, 220, 94–116.
    4. Chagas-Paula, D.A.; Oliveira, R.B.; Rocha, B.A.; Da Costa, F.B. Ethnobotany, chemistry, and biological activities of the genus Tithonia (Asteraceae). Chem. Biodivers. 2012, 9, 210–235.
    5. Baruah, N.C.; Sharma, R.P.; Madhusudanan, K.P.; Thyagarajan, G. Sesquiterpene lactones of Tithonia diversifolia. Stereochemistry of the tagitinins and related compounds. J. Org. Chem. 1979, 44, 1831–1835.
    6. Zhao, G.; Li, X.; Chen, W.; Xi, Z.; Sun, L. Three new sesquiterpenes from Tithonia diversifolia and their anti-hyperglycemic activity. Fitoterapia 2012, 83, 1590–1597.
    7. Ajao, A.A.; Moteetee, A.N. Tithonia diversifolia (Hemsl) A. Gray. (Asteraceae: Heliantheae), an invasive plant of significant ethnopharmacological importance: A review. South Afr. J. Bot. 2017, 113, 396–403.
    8. Jama, B.; Palm, C.A.; Buresh, R.J.; Niang, A.; Gachengo, C.; Nziguheba, G.; Amadalo, B. Tithonia diversifolia as a green manure for soil fertility improvement in western Kenya: A review. Agrofor. Syst. 2000, 49, 201–221.
    9. Hahn-Von-Hessberg, C.M.; Grajales-Quintero, A.; Narváez-Solarte, W. Apparent digestibility coefficient of common fodder plants in the andean region for the feeding of nile tilapia (Oreochromis niloticus). Inf. Technol. 2016, 27, 63–72.
    10. Tembo, Y.; Mkindi, A.G.; Mkenda, P.A.; Mpumi, N.; Mwanauta, R.; Stevenson, P.C.; Ndakidemi, P.A.; Belmain, S.R. Pesticidal plant extracts improve yield and reduce insect pests on legume crops without harming beneficial arthropods. Front. Plant Sci. 2018, 9, 1425.
    11. Adekiya, A.O. Green manures and poultry feather effects on soil characteristics, growth, yield, and mineral contents of tomato. Sci. Hortic. 2019, 257, 108721.
    12. Muoghalu, J.I.; Chuba, D.K. Seed germination and reproductive strategies of Tithonia diversifolia (Hemsl.) Gray and Tithonia rotundifolia (P.M) Blake. Appl. Ecol. Environ. Res. 2005, 3, 39–46.
    13. Tongma, S.; Kobayashi, K.; Usui, K. Allelopathic activity of Mexican sunflower (Tithonia diversifolia (Hemsl.) A. Gray) in soil under natural field conditions and different moisture conditions. Weed Biol. Manag. 2001, 1, 115–119.
    14. Miranda, M.A.F.M.; Varela, R.M.; Torres, A.; Molinillo, J.M.G.; Gualtieri, S.C.J.; Macías, F.A. Phytotoxins from Tithonia diversifolia. J. Nat. Prod. 2015, 78, 1083–1092.
    15. Suzuki, M.; Iwasaki, A.; Suenaga, K.; Kato-Noguchi, H. Phytotoxic property of the invasive plant Tithonia diversifolia and a phytotoxic substance. Acta Biol. Hung. 2017, 68, 187–195.
    16. Thompson, J.D.; McNeilly, T.; Gray, A.J. Population variation in Spartina anglica C.E. Hubbard. I. Evidence from a common garden experiment. New Phytol. 1991, 117, 115–128.
    17. Mack, R.M. Predicting the identity and fate of plant invaders: Emergent and emerging approaches. Biol. Conserv. 1996, 78, 107–121.
    18. Cappuccino, N.; Arnason, J.T. Novel chemistry of invasive exotic plants. Biol. Lett. 2006, 2, 189–193.
    19. Yang, J.; Tang, L.; Guan, Y.-L.; Sun, W.-B. Genetic diversity of an alien invasive plant Mexican sunflower (Tithonia diversifolia) in China. Weed Sci. 2012, 60, 552–557.
    20. Sampaio, B.L.; Edrada-Ebel, R.; Da Costa, F.B. Effect of the environment on the secondary metabolic profile of Tithonia diversifolia: A model for environmental metabolomics of plants. Sci. Rep. 2016, 6, 29265.
    21. Cappuccino, N.; Carpenter, D. Invasive exotic plants suffer less herbivory than non-invasive plants. Biol. Lett. 2005, 1, 435–438.
    22. Keane, R.M.; Crawley, M.L. Exotic plant invasions and the enemy release hypothesis. Trends Ecol. Evol. 2002, 17, 164–170.
    23. Mitchell, C.E.; Power, A.G. Release of invasive plants from fungal and viral pathogens. Nature 2003, 421, 625–627.
    24. Ambrósio, S.R.; Oki, Y.; Heleno, V.C.G.; Chaves, J.S.; Nascimento, P.G.B.D.; Lichston, J.E.; Constantino, M.G.; Varanda, E.M.; Da Costa, F.B. Constituents of glandular trichomes of Tithonia diversifolia: Relationships to herbivory and antifeedant activity. Phytochemistry 2008, 69, 2052–2060.
    25. Pavela, R.; Dall′Acqua, S.; Sut, S.; Baldan, V.; Kamte, S.L.N.; Nya, P.C.B.; Cappellacci, L.; Petrelli, R.; Nicoletti, M.; Canale, A.; et al. Oviposition inhibitory activity of the Mexican sunflower Tithonia diversifolia (Asteraceae) polar extracts against the two-spotted spider mite Tetranychus urticae (Tetranychidae). Physiol. Mol. Plant Pathol. 2018, 101, 85–92.
    26. Kerebba, N.; Oyedeji, O.O.; Byamukama, R.; Kuria, S.K. Pesticidal activity of Tithonia diversifolia (Hemsl.) A. Gray and Tephrosia vogelii (Hook f.); phytochemical isolation and characterization: A review. South Afr. J. Bot. 2019, 121, 366–376.
    27. Venâncio, H.; Bianchi, R.A.; Lobato, T.O.S.; Sampaio, M.V.; Santos, J.C. Tritrophic interaction between the Mexican sunflower, the aphid aphis gossypii and natural enemies in a greenhouse experiment. Acta Sci. Biol. Sci. 2020, 42, e47120.
    28. Callaway, R.M.; Aschehoug, E.T. Invasive plants versus their new and old neighbors: A mechanism for exotic invasion. Science 2000, 290, 521–523.
    29. Callaway, R.M.; Ridenour, W.M. Novel weapons: Invasive success and the evolution of increased competitive ability. Front. Ecol. Environ. 2004, 2, 419–426.
    30. Chengxu, W.; Mingxing, Z.; Xuhui, C.; Bo, Q. Review on allelopathy of exotic invasive plants. Procedia Eng. 2011, 18, 240–246.
    31. Meiners, S.J.; Kong, C.H.; Ladwig, L.M.; Pisula, N.L.; Lang, K.A. Developing an ecological context for allelopathy. Plant Ecol. 2012, 213, 1861–1867.
    32. Lockwood, J.L.; Simberloff, D.; McKinney, M.L.; Von Holle, B. How many, and which, plants will invade natural areas. Biol. Invasions 2001, 3, 1–8.
    33. Belz, R.G. Allelopathy in crop/weed interactions—An update. Pestic. Manag. Sci. 2007, 63, 308–326.