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Table of Contents

    Topic review

    PRKAR1A and Thyroid Tumors

    Subjects: Oncology
    View times: 15
    Submitted by: Fabio Faucz


    Thyroid cancer is the most common endocrine tumor in the general population and the incidence continues to rise in the United States. The American Cancer Society estimates that there will be 44,280 new cases of thyroid cancer (12,150 in men and 32,130 in women) and about 2200 deaths (1050 in men and 1150 in women) in the United States in 2021. The increased incidence could be possibly attributed to the increased detection of these tumors with imaging technics (like ultrasound and computed tomography (CT)) that better characterize incidental findings of small thyroid nodules.

    1. Introduction

    In the majority of patients (about 90%), well-differentiated epithelial thyroid cancer is present; this is further categorized into papillary thyroid cancer (PTC) and follicular thyroid cancer (FTC), based on histological criteria [1][2]. The long-term survival of those patients is excellent, with 5-year relative survival rate (as of 2010–2016) being as high as 98% in all stages (>99% for local tumors and 55% for tumors with distant metastases) [3]. However, FTC tends to behave more aggressively with distant metastases and vascular invasion [4][5] being more common and thus its prognosis is poorer than PTC [6]. The rest of the thyroid carcinomas (~2–3%) include medullary thyroid carcinomas (MTCs) that originate from the calcitonin-producing parafollicular C cells, while anaplastic carcinomas (ATCs) and poorly differentiated carcinomas account for the remaining 7–8% [2]. In addition to the above tumors, benign thyroid tumors that usually present as thyroid nodules as well, include benign hyperplasia or benign follicular adenomas [1].

    Thyroid malignancies are also associated with at least two syndromes with inherited tumor predisposition, Cowden syndrome (CS, OMIM# 158350) and Carney Complex (CNC, OMIM #160980). CS is a multiple hamartoma syndrome, including FTC, brain and breast cancer. It is caused by inactivating mutations in the PTEN gene, a dual-specificity phosphatase that negatively regulates PI3 Kinase/AKT pathway; mutations in this gene have been detected in 5% of FTCs [7]; however, a mouse harboring a deletion of Pten in the thyroid developed thyroid hyperplasia and not FTC [8].

    2. Medullary Thyroid Cancer as Part of MEN2 Syndromes

    About one-third of MTCs are hereditary, presenting as multicentric and bilateral, in contrast with sporadic cases that are a single unilateral tumor [9][10]. They present as part of MEN2A (70–80%), MEN2B (5%), or familial MTC (FMTC) (10–20%). The first inherited subtype of MTC, MEN2A, consists of primary hyperparathyroidism, pheochromocytoma and MTC in which it can occur early in life (approximately 5 years of age) in contrast with sporadic cases that presents between 15 and 20 years [10][11]. MEN2B is characterized by pheochromocytoma, MTC and non-endocrine diseases such as mucosal neuromas, intestinal tumors (most commonly ganglioneuromas) and Marfanoid habitus [11]. In the case of FMTC, only the thyroid gland is affected, but in a significant number of relatives in the same family, usually between the ages of 20 and 40 [11][12][13]. Activating germline RET mutations have been identified as the main cause of up to 98% of hereditary MTCs and up to half of sporadic cases [14]. Depending on the mutated residue within the RET protein, the phenotype may differ [15][16][17][18]. Families with two or more members with MTC are referred for genetic counseling and screening, if positive they undergo further testing for hyperparathyroidism and pheochromocytoma [3][19][20]. In the case of sporadic MTCs, somatic RET mutations, particularly M918T, has been shown to be associated with more aggressive disease and worse prognosis [20][21].

    3. Anaplastic Thyroid Carcinoma

    ATC is a rare (1–2%) but very aggressive type of thyroid cancer [22] with average age at diagnosis over 70 years [23]. It is considered to evolve from dedifferentiation of a pre-existing DTC caused by accumulation of several genetic alterations that lead to disruption of two signaling pathways that are involved in cell proliferation, PI3K-AKT and MAPK [24][25][26]. The most common mutations include TP53 , which is considered a genetic hallmark of ATC, as well as RAS , BRAF , PIK3CA [27][28], mutations that have also been identified in DTC [29]. Median survival is usually less than 6 months after diagnosis and the mortality rate is >90% [30][31]. Due to its extremely aggressive nature, it is critical to be diagnosed promptly. Clinical symptoms are usually used for the diagnosis, in contrast with DTC in which diagnosis is made by FNA of a suspicious nodule [23]. The symptoms can last from 4 weeks to 11 months and usually consist of a rapidly enlarging neck mass along with vocal cord paralysis and dyspnea [23].

    4. Systemic Treatments for Thyroid Cancer

    Two multikinase inhibitors (MKI), lenvatinib and sorafenib, are currently approved by the US Food and Drug Administration (FDA) for the treatment of advanced DTC. Sorafenib was approved based on the favorable results of a placebo-controlled phase 3 clinical trial (DECISION) [32]. The positive results of the lenvatinib phase 3 SELECT trial [33] as well as a phase 2 study led to the approval of that drug [34]. Cabozatinib and vandetanib are approved by the FDA for the treatment of MTC. Vandetanib is approved for symptomatic, unresectable, locally advanced, or metastatic MTC in patients based on a phase 3 trial (ZETA) [35]. Cabozantinib was studied in a phase 3 clinical trial (EXAM) [36] and showed good results while another clinical trial in MTC patients is still active (EXAMINER, NCT01896479). RET-inhibitors have been studied as well for thyroid cancers that harbor RET mutations (NCT03157128, NCT04211337, NCT03906331, NCT04280081, NCT03037385).

    4.1. Immunotherapy

    In the recent years, immunotherapy has emerged as a new transformative approach into the body’s natural antitumor defenses. To date, there is no approved immunotherapy for advanced thyroid cancer. A few clinical trials using novel immunotherapy agents like programmed cell death protein 1 (PD-1) checkpoint inhibitors are ongoing. Pembrolizumab in an Ib phase trial (KEYNOTE) showed a tumor size reduction of 35–50% in PTC and FTC. The use of another anti-PD1 agent (spartalizumab) was evaluated in progressive ATC that responded to therapy [37]. In an ongoing phase 2 clinical trial (NCT03246958), the efficacy of the combination of nivolumab (anti-PD1-1) and ipilimumab (anti-CTLA-4- cytotoxic T-lymphocyte-associated protein 4) was evaluated in patients with aggressive thyroid cancer. In addition, multiple clinical trials with VEGF and/or VEGF inhibitor and immune checkpoint inhibitors have been designed. Pemproblizumab plus lenvatinib was investigated in a phase 2 trial for unresectable ATC (NCT04171622) as well as in a randomized study in a small group of advanced ATC and PDTC [38]. The same combination is under study in DTC and PDTC naïve or progressing after lenvatinib patients (NCT02973997). Triple combined therapy (cabozantinib plus nivolumab and ipilimumab) is under evaluation for DTC and PDTC (NCT03914300).

    4.2. Treatment for PRKAR1A-Associated Thyroid Tumors

    To date, there is no medical treatment targeting cAMP/PKA signaling in CNC. Surgical treatment is the treatment of choice in patients with PRKAR1A-associated thyroid tumor [39].

    5. Clinical Surveillance in Patients with PRKAR1A-Associated Thyroid Tumors

    Human studies in CNC underly the importance of investigating thyroid nodules in these patients. Multiple thyroid nodules are present in up to 75% of patients with CNC on thyroid ultrasound; the majority of them are non-functioning follicular adenomas [40]. However, thyroid carcinomas are common as well. Early detection is vital and CNC patients should be followed with long-term clinical and/or ultrasound surveillance with biopsy of suspicious nodules, for early detection of carcinomas [40].

    Because CNC is inherited in an autosomal dominant manner, each child of an affected individual has a 50% chance of inheriting the pathogenic variant. Most of the affected patients (approximately 70%) have an affected parent. In the case that the pathogenic variant is known in a family, prenatal testing may be recommended [40].

    6. Conclusions

    In summary, recent advances in molecular mechanisms of thyroid cancer have improved cancer prognosis and detection. PRKAR1A, a regulator of PKA activity, is possibly involved in the molecular events that contribute to thyroid cancer. Identifying the genetic basis of PRKAR1A-associated thyroid tumors is important as it will provide better clinical management to these patients.

    The entry is from 10.3390/cancers13153834


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