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    Topic review

    Chitosan as Coating for Biocontrol

    Subjects: Biology
    View times: 14

    Definition

    This entry focused on the scientific production, trends, and characteristics of a knowledge domain of high worldwide importance, namely, the use of chitosan as a coating for postharvest disease biocontrol in fruits and vegetables, which are generated mainly by fungi and bacteria such as Aspergillus niger, Rhizopus stolonifera, and Botrytis cinerea. For this, the analysis of 875 published documents in the Scopus database was performed for the years 2011 to 2021. The information of the keywords’ co-occurrence was visualized and studied using the free access VOSviewer software to show the trend of the topic in general.

    1. Introduction

    The most effective and used edible coatings for the protection of fruits and vegetables are made up one or more natural polymers such as cellulose [1], alginate [2], gellan [3], pectin, starch and its derivatives [4], methylcellulose, carboxymethylcellulose [5], Arabic gum [6], whey protein concentrate [7], and chitosan or chitosan nanoemulsion [8][9]. Chitosan is a deacetylated form of chitin, (poly β-(1→4) N-acetyl-d-glucosamine), and is the second most abundant biopolymer found in nature after cellulose, with prominent film-forming properties, non-toxicity, biodegradable and biocompatible properties, high mechanical strength, and excellent antimicrobial activity [10], and it has been used as a coating in various foods [11]. Furthermore, chitosan has been approved by the United States Food and Drug Administration (USFDA) as a food additive and listed as generally recognized as safe (GRAS) in the USA and Japan

    In the last years, chitosan has gained more attention from researchers due to its broad-spectrum activity and high destruction rate against Gram-positive and Gram-negative bacteria [12] and filamentous fungi [13][14][15]. However, its quality, chemical and biological properties, and therefore its applications are closely related to numerous intrinsic and extrinsic factors such as the degree of deacetylation (DD) Chitosan is a polysaccharide mainly obtained from invertebrates; insect cuticles; fungal cell walls; green algae; yeast; crustacean shells such as those of cockles, shrimp, crabs, etc.; by chemical (alkaline hydrolysis with NaOH solutions) and other less-used methods such as alkaline treatment at high temperature and high pressure [16]; or a process that uses ultrasound [17][18][19] and sometimes, less frequently, enzymatic deacetylation [20]. Moreover, it is important to mention that the method used increases or decreases the deacetylation degree (DD) and determines the content of free amino groups and the cationic character [21].

    On the other hand, the DD grade of chitosan indicates the number of acetyl groups removed from chitin, which corresponds to the release of the amino groups from the N-acetylglucosamine monomers. In this regard, He et al. [22] mentioned the chitosan classification according to its DD as being low between 55 and 70%, medium between 70 and 85%, high between 85 and 95%, and ultra-high between 95 and 100% [22][23]. [24] found that the chitosan microspheres with a 63.6% degree of deacetylation (DD) exhibited the highest antibacterial activity concerning the microspheres made with chitosan at 83.7% of DD, which exerted at least some antibacterial activity.

    Furthermore, although the antifungal or antimicrobial mechanism of action of chitosan is still being studied, some hypotheses have already been proposed, e.g., Yang [25] mentioned that this polymer permeates and perforates the fungus nuclei, the protein cell membranes, and intracellular constituents, inactivating bacterial metabolism due to the presence of an amino group that has a positive charge with a pH lower than 6.3 and interacts with negative charges of the cell wall of microorganisms, generating the rupture or lysis of these structures, causing the loss of protein compounds and other intracellular constituents [26]. However, because there are still many factors that need to be analyzed during the coating process such as the coating suspensions properties, the fruit surface microstructure and wettability [1], and the synergy between biocontrol agents and natural bioactive compounds, it is necessary that more studies be conducted [27].

    On the other hand, due to the vast amount of existing information, an “intoxication” problem can be generated, as reported by Flórez-Martínez et al. (2021) [28]. Consequently, in this paper, we carried out a bibliometric analysis to show and quantify the evolution of the research, perspectives, challenges, and prospects, not only a cross-sectional study, as this provides limited information.

    2. Bibliometric Analysis

    For this, a Boolean search string was used. First, the search was realized for those coming from the relevant keyword fields (e.g., chitosan, postharvest, biocontrol, fungi, and phytopathogens). Subsequently, the Scopus service was used, with the option to combine searches, using the “Combine queries” field, where the syntax applied is the # symbol with the “OR” and “AND” operators. AND (EXCLUDE (EXACTKEYWORD, “Biomedical Applications”)); in order to limit the topic additional phrases, (AND (LIMIT-TO (EXACTKEYWORD, “Fruits”))) was added into the query string, which resulted in 92 articles [29][30].

    The total number of searches resulted in 875 publications (from January 2011 to 14 April 2021). The raw data obtained (CSV Format) from Scopus was analyzed using the VOSviewer software (www.vosviewer.com, accessed on 25 March 2021; Van Eck and Waltman, 2009–2020, version 1.6.15, Leiden University, Leiden, The Netherlands) for the construction and bibliometric visualization of networks of institutions, countries, keywords, and citations per article.

    San Jose, CA, USA) was used to design and produce the graph shown in Figure 1; then, the “Dynamic fit wizard” plugin was applied for curve fitting, using a linear regression model. The obtained equation was Equation (1). (1)f=y0+a∗x where Y = response (number of published papers);

    3. Results

    Figure 1 shows the number of scientific publications per year, limiting this research to a period from January 2011 to 14 April 2021. It can be observed that the temporal evolution between the number of articles versus years, in this field of research, has had linear growth in the last decade. From 2011 to 2013, few documents per year were published on the subject (11, 34, and 41, respectively), but these have increased. In order to perform the data analysis and have it be explained by a linear regression model using the best fit (R2 = 0.9859) of the results, we did not consider this year and only included studies until the year 2020, as shown in Figure 1.

    In all published documents, the central focus of an article is highlighted using keywords, which are essential and facilitate mapping for readers [29] so that their analysis is necessary. One method is by word cloud (Figure 2), which provides a first view of the dataset, allowing us to explore and visually analyze, as well as to size and create the first classification for our data. The size of the words “chitosan” and “coatings” suggests that in most research, these words have been the most persistent theme. However, this technique only provides qualitative information, and therefore it is necessary to execute a more in-depth analysis.

    A more accurate method is the co-keyword cluster mapping (Figure 3), obtained from author keywords. Here, the software (VOSviewer) analyzed the 84 most frequently terms, and each one of them was repeated at least six times. It is imperative to mention that each circle represents a keyword, and its size indicates its appearance frequency in the articles. Data analysis generated nine clusters marked with different colors, e.g., the first cluster (in red) contained 19 terms, with “chitosan” being the most frequent term, with a greater node keyword, closely associated with the 81 terms belonging at the nine clusters, but mainly with largest nodes such as “edible coating, coating, shelf life, antibacterial activity”.

    It is important to note that the node’s color also determined when the term or keyword was introduced for the first time in the network [14]. These results could be significant for the scientific development research that involves the topic of food waste that generates so many economic losses. For example, the generation of coatings involved added nanoparticles for protection and longer shelf life of different foods. However, much research is still needed to establish the existing interactions between food matrices and these coatings.

    On the other hand, as shown in Figure 4, the keyword “chitosan”, highlighted with the larger circle in blue, also determined a central position, indicating its importance and direct connection with other smaller nodes such as “fruit coating”, “useful life”, “strawberry”, “mango”, “guava”, “tomato”, and “papaya”. Terms that gain importance as will be seen later.

    On the other hand, Table 1 shows the top 20 most-cited articles, extracted from the search of 875 documents. Obtained data such as the year of publication, authorship, journal title, publication count, and citation count were analyzed. Due to the high quantity of published papers, our analysis focused on the most highly cited papers and those related to the keyword “fruits”, which generated 92 documents, analyzed as described in Table 2, Table 3 and Table 4.

    The top of 20 most cited authors and documents between 2011 and 2021.

    This study was published in Scientific Reports and intended to explore the interaction pattern role of the iron oxide nanoparticle (IONP)–bacteria interface that enhances the antimicrobial activity of IONP using positively charged chitosan. In analyzing the rest of the authors and the most cited scientific papers in the domain under study, we noted the importance of the use of chitosan and its multiple applications as well as their effect as a coating in various fruits. In this sense, the second most cited document [31] reported the use of chitosan as an effective control in reducing weight loss, maintaining firmness, delayed changes in the peel color, and soluble solids in papaya (Carica papaya L.), which is one of the most important fruit crops in the world and has a short post-harvest life. However, it did not study the damage by opportunistic plant pathogens capable of producing diseases or loss of crops, which has led to countless studies, as shown in Table 2, Table 3 and Table 4.

    It is worth noting that the emerging interdisciplinary field of nanotechnology has been a recurring phenomenon in recent studies, as shown in Table 1, with the highest cited document or the documents published by various authors [32][33][34][35].

    Exceptionally, the co-occurrence between keywords allows for the generation of knowledge in search of a common goal. Over the past decade, researchers around the world have developed many different methods to minimize postharvest fruit loss because they have the highest waste rates of any food product (45% waste [36]), which is a global problem. A novel method is the use of chitosan coatings as well as their different combinations with other polymers or with essential oils or nanoparticles, among others, as shown in Table 2. This allows for the storage period to be increased in order to postpone the deterioration of fruits and vegetables and preventing the growth of microorganisms transmitted by food on the surfaces of the products.

    Fungi that affect postharvest fruit quality: analysis from 2011 to 2021.

    The letters correspond to the fungi worked by each author: Letter A, B, and C correspond to [13]; letter B corresponds to [37], and letter C corresponds to [1].

    It was observed that there is plenty of research involving published studies concerning the antimicrobial and antifungal activity of chitosan as well as a combination with other polymers or the application of different essential oils against foodborne pathogens. However, of the total reports (875 documents published), only 93 documents with the keyword “fruits” were analyzed due to the importance of this kind of food. The findings mentioned below and those in Table 2, Table 3, Table 4 and Table 5 correspond to these documents.

    Published results of bacterial contamination by different microorganisms in fruits: from 2011 to 2021.

    Published results from bacterial and fungal contamination by different microorganisms in fruits: from 2011 to 2021.

    Recently, the impact of preharvest foliar spraying with chitosan and postharvest aloe vera gel coating (AVG) on the quality of table grapes during storage was evaluated, thereby extending the shelf life of the fruit up to 15 days by significantly reducing the decomposition index [38]. Another recent finding is the production of edible coating films based on Pickering emulsions, which showed a smaller droplet size, narrower size distribution, and improved stability. These could inhibit the growth of typical spoilage organisms such as S. aureus and E. coli in order to preserve fruits and vegetables [39]. [40] applied this method by adding oleic acid and cellulose nanocrystal in “Bartlett” pears (Pyrus communis L.) for delaying ripening and superficial scald during the long-term cold storage.

    Table 2, Table 3 and Table 4 show studies concerning the application of chitosan as an antimicrobial and antifungal to maintain fruit and vegetable quality at the postharvest stage. It is highlighted that several studies have focused on reducing the antifungal activity of Aspergillus niger, Rhizopus stolonifera, Botrytis cinerea, P. expansum, Alternaria alternate, Colletotrichum gloeosporioides, etc. In some studies, the antifungal activity of chitosan depends on the extraction procedure, the deacetylation percentage, molecular weight, or the microstructure of the fruit and the interaction of the coating material.

    Some fruits such as strawberries [1][13][41][42][37][43][44][45][46][47][48], mango (Mangifera indica L.) , tomato [49][50][51][52][53][54][55][56][57], guava [58][59][60], banana [6][61][62], apples [63][64][65][66], or fresh-cut apple slices [67][68] are especially perishable and therefore there is a larger number of documents focused on decreasing mechanical injury, desiccation, decay, and physiological disorders during storage [41], as observed in Table 2, Table 3, Table 4 and Table 5. In this sense, the most cited document (166 citations) [41] mentioned that the use of chitosan functionalized by acylation with palmitoyl chloride increase its hydrophobicity in order to ensure a controlled release and improve its stability and adherence in strawberries. Notwithstanding, research in this area is still incipient, and therefore is necessary to carry out future research about the topic with other fruits or in different matrixes of food.

    For this purpose, mixtures of chitosan and some other materials have also been used, as shown in Table 5; these results indicated that the coatings could reduce the damage in different fruits or vegetables.

    Moreover, other studies have addressed that the chitosan coating applied in pummelo fruit mitigates the development of juice sac granulation and delays postharvest senescence in the same fruit during room temperature storage [69], and in eggplant cultivars (purple long, purple round, and white long), chitosan was effective in minimizing weight loss, maintained quality, and prolonged storability with good appearance and overall acceptability [70]. However, it is necessary to conduct more research focused on combinations of adequate techniques and different coating materials that consider the intrinsic and extrinsic factors that affect food, as well as allowing for enhancement of shelf life and decreases in the amount of waste.

    Coating materials mixture with chitosan applied to extend the shelf-life and improve the quality of fruits.

    4. Conclusions

    This bibliometric review analyzed the evolutionary process over the past decade of topics about chitosan as coating and their fruits and vegetables’ antifungal or antimicrobial effects. VOSviewer software is a useful and versatile tool that allows for easy visualization and analysis of bibliometric networks. In this paper, 875 documents reported that coatings made of chitosan only or chitosan in combination with other biopolymers are a natural and safe post-harvest biocontrol strategy to decrease microbial spoilage mainly by pre- Finally, this work can provide a useful perspective for future research in the studied field since it demonstrates the existence of an emerging area of study that is intended to reduce a global problem caused by the generation of agro-industrial waste due to the loss of post-harvest damaged crops.

    The entry is from 10.3390/membranes11060421

    References

    1. Sun, X.; Wu, Q.; Picha, D.H.; Ferguson, M.H.; Ndukwe, I.E.; Azadi, P. Comparative performance of bio-based coatings formulated with cellulose, chitin, and chitosan nanomaterials suitable for fruit preservation. Carbohydr. Polym. 2021, 259, 117764.
    2. Arroyo, B.J.; Bezerra, A.C.; Oliveira, L.L.; Arroyo, S.J.; de Melo, E.A.; Santos, A.M.P. Antimicrobial active edible coating of alginate and chitosan add ZnO nanoparticles applied in guavas (Psidium guajava L.). Food Chem. 2020, 309, 125566.
    3. Morsy, N.; Rayan, A.M. Effect of different edible coatings on biochemical quality and shelf life of apricots (Prunus armenica L. cv Canino). J. Food Meas. Charact. 2019, 13, 3173–3182.
    4. Bello-Lara, J.E.; De Nayarit, U.A.; Balois-Morales, R.; Juarez-Lopez, P.; Alia-Tejacal, I.; Peña-Valdivia, C.B.; Jiménez-Zurita, J.O.; Sumaya-Martínez, M.T.; Jiménez-Ruíz, E.I.; Universidad Autónoma del Estado de Morelos; et al. Coatings based on starch and pectin from ‘Pear’ banana (Musa ABB), and chitosan applied to postharvest ‘Ataulfo’ mango fruit. Rev. Chapingo Ser. Hortic. 2016, 22, 209–218.
    5. Baswal, A.K.; Dhaliwal, H.S.; Singh, Z.; Mahajan, B.; Kalia, A.; Gill, K.S. Influence of carboxy methylcellulose, chitosan and beeswax coatings on cold storage life and quality of Kinnow mandarin fruit. Sci. Hortic. 2020, 260, 108887.
    6. La, D.D.; Nguyen-Tri, P.; Le, K.H.; Nguyen, P.T.; Nguyen, M.D.-B.; Vo, A.T.; Chang, S.W.; Tran, L.D.; Chung, W.J.; Nguyen, D.D. Effects of antibacterial ZnO nanoparticles on the performance of a chitosan/gum arabic edible coating for post-harvest banana preservation. Prog. Org. Coat. 2021, 151, 106057.
    7. Simonaitiene, D.; Brink, I.; Šipailienė, A.; Leskauskaite, D. The effect of chitosan and whey proteins-chitosan films on the growth ofPenicillium expansumin apples. J. Sci. Food Agric. 2014, 95, 1475–1481.
    8. Aparicio-García, P.F.; Ventura-Aguilar, R.I.; Del Río-García, J.C.; Hernández-López, M.; Guillén-Sánchez, D.; Salazar-Piña, D.A.; Ramos-García, M.d.L.; Bautista-Baños, S. Edible Chitosan/Propolis Coatings and Their Effect on Ripening, Development of Aspergillus flavus, and Sensory Quality in Fig Fruit, during Controlled Storage. Plants 2021, 10, 112.
    9. Gull, A.; Bhat, N.; Wani, S.M.; Masoodi, F.A.; Amin, T.; Ganai, S.A. Shelf life extension of apricot fruit by application of nanochitosan emulsion coatings containing pomegranate peel extract. Food Chem. 2021, 349, 129149.
    10. Hosseinnejad, M.; Jafari, S.M. Evaluation of different factors affecting antimicrobial properties of chitosan. Int. J. Biol. Macromol. 2016, 85, 467–475.
    11. Kritchenkov, A.S.; Egorov, A.R.; Kurasova, M.N.; Volkova, O.V.; Meledina, T.V.; Lipkan, N.A.; Tskhovrebov, A.G.; Kurliuk, A.V.; Shakola, T.V.; Dysin, A.P.; et al. Novel non-toxic high efficient antibacterial azido chitosan derivatives with potential application in food coatings. Food Chem. 2019, 301, 125247.
    12. Kong, M.; Chen, X.G.; Xing, K.; Park, H.J. Antimicrobial properties of chitosan and mode of action: A state of the art review. Int. J. Food Microbiol. 2010, 144, 51–63.
    13. Melo, N.F.C.B.; de Lima, M.A.B.; Stamford, T.L.M.; Galembeck, A.; Flores, M.A.; Takaki, G.M.D.C.; Medeiros, J.A.D.C.; Stamford-Arnaud, T.M.; Stamford, T.C.M. In vivo and in vitro antifungal effect of fungal chitosan nanocomposite edible coating against strawberry phytopathogenic fungi. Int. J. Food Sci. Technol. 2020, 55, 3381–3391.
    14. Barbosa, M.W. Uncovering research streams on agri-food supply chain management: A bibliometric study. Glob. Food Secur. 2021, 28, 100517.
    15. Istúriz-Zapata, M.; Hernández-López, M.; Correa-Pacheco, Z.; Barrera-Necha, L. Quality of cold-stored cucumber as affected by nanostructured coatings of chitosan with cinnamon essential oil and cinnamaldehyde. LWT 2020, 123, 109089.
    16. Abdou, E.S.; Nagy, K.S.; Elsabee, M.Z. Extraction and characterization of chitin and chitosan from local sources. Bioresour. Technol. 2008, 99, 1359–1367.
    17. Kittur, F.S.; Harish Prashanth, K.V.; Udaya Sankar, K.; Tharanathanm, R.N. Characterization of chitin, chitosan and their carboxymethyl derivatives by differential scanning calorimetry. Carbohydr. Polym. 2002, 49, 185–193.
    18. Berger, L.R.R.; Stamford, T.C.M.; De Oliveira, K.; Árabe, R.; Pessoa, A.D.M.P.; De Lima, M.A.B.; Pintado, M.M.; Câmara, M.P.S.; Franco, L.D.O.; Magnani, M.; et al. Chitosan produced from Mucorales fungi using agroindustrial by-products and its efficacy to inhibit Colletotrichum species. Int. J. Biol. Macromol. 2018, 108, 635–641.
    19. Gonil, P.; Sajomsang, W. Applications of magnetic resonance spectroscopy to chitin from insect cuticles. Int. J. Biol. Macromol. 2012, 51, 514–522.
    20. EL Knidri, H.; Dahmani, J.; Addaou, A.; Laajeb, A.; Lahsini, A. Rapid and efficient extraction of chitin and chitosan for scale-up production: Effect of process parameters on deacetylation degree and molecular weight. Int. J. Biol. Macromol. 2019, 139, 1092–1102.
    21. Weißpflog, J.; Vehlow, D.; Müller, M.; Kohn, B.; Scheler, U.; Boye, S.; Schwarz, S. Characterization of chitosan with different degree of deacetylation and equal viscosity in dissolved and solid state–Insights by various complimentary methods. Int. J. Biol. Macromol. 2021, 171, 242–261.
    22. He, X.; Li, K.; Xing, R.; Liu, S.; Hu, L.; Li, P. The production of fully deacetylated chitosan by compression method. Egypt. J. Aquat. Res. 2016, 42, 75–81.
    23. Tavares, L.; Flores, E.E.E.; Rodrigues, R.C.; Hertz, P.F.; Noreña, C.P.Z. Effect of deacetylation degree of chitosan on rheological properties and physical chemical characteristics of genipin-crosslinked chitosan beads. Food Hydrocoll. 2020, 106, 105876.
    24. Kong, M.; Chen, X.-G.; Xue, Y.-P.; Liu, C.; Yu, L.-J.; Ji, Q.-X.; Cha, D.S.; Park, H.J. Preparation and antibacterial activity of chitosan microshperes in a solid dispersing system. Front. Mater. Sci. China 2008, 2, 214–220.
    25. Yang, J.; Sun, J.; An, X.; Zheng, M.; Lu, Z.; Lu, F.; Zhang, C. Preparation of ferulic acid-grafted chitosan using recombinant bacterial laccase and its application in mango preservation. RSC Adv. 2018, 8, 6759–6767.
    26. Tantala, J.; Thumanu, K.; Rachtanapun, C. An assessment of antibacterial mode of action of chitosan on Listeria innocua cells using real-time HATR-FTIR spectroscopy. Int. J. Biol. Macromol. 2019, 135, 386–393.
    27. Yang, Y.; Ge, L. Sensor coating employed to preliminarily evaluate the banana ripeness. Colloids Surf. A Physicochem. Eng. Asp. 2021, 616, 126057.
    28. Flórez-Martínez, D.H.; Contreras-Pedraza, C.A.; Rodríguez, J. A systematic analysis of non-centrifugal sugar cane processing: Research and new trends. Trends Food Sci. Technol. 2021, 107, 415–428.
    29. Hamidah, I.; Pawinanto, R.E.; Mulyanti, B.; Yunas, J. A bibliometric analysis of micro electro mechanical system energy harvester research. Heliyon 2021, 7, e06406.
    30. Khudzari, J.M.; Kurian, J.; Tartakovsky, B.; Raghavan, G. Bibliometric analysis of global research trends on microbial fuel cells using Scopus database. Biochem. Eng. J. 2018, 136, 51–60.
    31. Ali, A.; Muhammad, M.T.M.; Sijam, K.; Siddiqui, Y. Effect of chitosan coatings on the physicochemical characteristics of Eksotika II papaya (Carica papaya L.) fruit during cold storage. Food Chem. 2011, 124, 620–626.
    32. Von Moos, N.; Slaveykova, V.I. Oxidative stress induced by inorganic nanoparticles in bacteria and aquatic microalgae–state of the art and knowledge gaps. Nanotoxicology 2013, 8, 605–630.
    33. Regiel-Futyra, A.; Kus-Liśkiewicz, M.; Sebastian, V.; Irusta, S.; Arruebo, M.; Stochel, G.; Kyzioł, A. Development of Noncytotoxic Chitosan–Gold Nanocomposites as Efficient Antibacterial Materials. ACS Appl. Mater. Interfaces 2015, 7, 1087–1099.
    34. De Faria, A.F.; Perreault, F.; Shaulsky, E.; Chavez, L.H.A.; Elimelech, M. Antimicrobial Electrospun Biopolymer Nanofiber Mats Functionalized with Graphene Oxide–Silver Nanocomposites. ACS Appl. Mater. Interfaces 2015, 7, 12751–12759.
    35. Perelshtein, I.; Ruderman, Y.; Perkas, N.; Tzanov, T.; Beddow, J.; Joyce, E.; Mason, T.J.; Blanes, M.; Mollá, K.; Patlolla, A.; et al. Chitosan and chitosan–ZnO-based complex nanoparticles: Formation, characterization, and antibacterial activity. J. Mater. Chem. B 2013, 1, 1968–1976.
    36. Kitinoja, L.; Kader, A. Measuring postharvest losses of fresh fruits and vegetables in developing countries. PEF White Pap. 2015, 15, 26.
    37. Khalifa, I.; Barakat, H.; El-Mansy, H.A.; Soliman, S.A. Effect of Chitosan-Olive Oil Processing Residues Coatings on Keeping Quality of Cold-Storage Strawberry (Fragaria ananassa. Var. Festival). J. Food Qual. 2016, 39, 504–515.
    38. Nia, A.E.; Taghipour, S.; Siahmansour, S. Pre-harvest application of chitosan and postharvest Aloe vera gel coating enhances quality of table grape (Vitis vinifera L. cv. ‘Yaghouti’) during postharvest period. Food Chem. 2021, 347, 129012.
    39. Xie, B.; Zhang, X.; Luo, X.; Wang, Y.; Li, Y.; Li, B.; Liu, S. Edible coating based on beeswax-in-water Pickering emulsion stabilized by cellulose nanofibrils and carboxymethyl chitosan. Food Chem. 2020, 331, 127108.
    40. Jung, J.; Deng, Z.; Zhao, Y. Mechanisms and performance of cellulose nanocrystals Pickering emulsion chitosan coatings for reducing ethylene production and physiological disorders in postharvest ‘Bartlett’ pears (Pyrus communis L.) during cold storage. Food Chem. 2020, 309, 125693.
    41. Vu, K.; Hollingsworth, R.; Leroux, E.; Salmieri, S.; Lacroix, M. Development of edible bioactive coating based on modified chitosan for increasing the shelf life of strawberries. Food Res. Int. 2011, 44, 198–203.
    42. Wang, S.Y.; Gao, H. Effect of chitosan-based edible coating on antioxidants, antioxidant enzyme system, and postharvest fruit quality of strawberries (Fragaria × aranassa Duch.). LWT 2013, 52, 71–79.
    43. Ventura-Aguilar, R.; Bautista-Baños, S.; Flores-García, G.; Zavaleta-Avejar, L. Impact of chitosan based edible coatings functionalized with natural compounds on Colletotrichum fragariae development and the quality of strawberries. Food Chem. 2018, 262, 142–149.
    44. Duran, M.; Aday, M.S.; Zorba, N.N.D.; Temizkan, R.; Büyükcan, M.B.; Caner, C. Potential of antimicrobial active packaging ‘containing natamycin, nisin, pomegranate and grape seed extract in chitosan coating’ to extend shelf life of fresh strawberry. Food Bioprod. Process. 2016, 98, 354–363.
    45. Valenzuela, C.; Tapia, C.; López, L.; Bunger, A.; Escalona, V.; Abugoch, L. Effect of edible quinoa protein-chitosan based films on refrigerated strawberry (Fragaria × ananassa) quality. Electron. J. Biotechnol. 2015, 18, 406–411.
    46. Muley, A.B.; Singhal, R.S. Extension of postharvest shelf life of strawberries (Fragaria ananassa) using a coating of chitosan-whey protein isolate conjugate. Food Chem. 2020, 329, 127213.
    47. Benhabiles, M.S.; Drouiche, N.; Lounici, H.; Pauss, A.; Mameri, N. Effect of shrimp chitosan coatings as affected by chitosan extraction processes on postharvest quality of strawberry. J. Food Meas. Charact. 2013, 7, 215–221.
    48. Perdones, Á.; Escriche, I.; Chiralt, A.; Vargas, M. Effect of chitosan–lemon essential oil coatings on volatile profile of strawberries during storage. Food Chem. 2016, 197, 979–986.
    49. Robledo, N.; Vera, P.; López, L.; Yazdani-Pedram, M.; Tapia, C.; Abugoch, L. Thymol nanoemulsions incorporated in quinoa protein/chitosan edible films; antifungal effect in cherry tomatoes. Food Chem. 2018, 246, 211–219.
    50. Cui, H.; Yuan, L.; Li, W.; Lin, L. Edible film incorporated with chitosan andArtemisia annuaoil nanoliposomes for inactivation ofEscherichia coliO157:H7 on cherry tomato. Int. J. Food Sci. Technol. 2017, 52, 687–698.
    51. Peralta-Ruiz, Y.; Tovar, C.D.G.; Sinning-Mangonez, A.; Coronell, E.A.; Marino, M.F.; Chaves-Lopez, C. Reduction of Postharvest Quality Loss and Microbiological Decay of Tomato “Chonto” (Solanum lycopersicum L.) Using Chitosan-E Essential Oil-Based Edible Coatings under Low-Temperature Storage. Polymers 2020, 12, 1822.
    52. Araújo, J.M.S.; De Siqueira, A.C.P.; Blank, A.F.; Narain, N.; de Aquino Santana, L.C.L. A Cassava Starch–Chitosan Edible Coating Enriched with Lippia sidoides Cham. Essential Oil and Pomegranate Peel Extract for Preservation of Italian Tomatoes (Lycopersicon esculentum Mill.) Stored at Room Temperature. Food Bioprocess. Technol. 2018, 11, 1750–1760.
    53. Breda, C.A.; Morgado, D.L.; De Assis, O.B.G.; Duarte, M.C.T. Effect of chitosan coating enriched with pequi (Caryocar brasiliense Camb.) peel extract on quality and safety of tomatoes (Lycopersicon esculentum Mill.) during storage. J. Food Process. Preserv. 2017, 41, e13268.
    54. Pagno, C.H.; Castagna, A.; Trivellini, A.; Mensuali-Sodi, A.; Ranieri, A.; Ferreira, E.A.; Rios, A.D.O.; Flôres, S.H. The nutraceutical quality of tomato fruit during domestic storage is affected by chitosan coating. J. Food Process. Preserv. 2017, 42, e13326.
    55. Ruzaina, I.; Rashid, N.A.; Jia, W.; Som, H.Z.M.; Seng, C.C.; Sikin, A.M.; Wahab, N.A.; Abidin, M.Z.; Zhong, F.; Li, Y. Effect of Different Degree of Deacetylation, Molecular Weight of Chitosan and Palm Stearin and Palm Kernel Olein Concentration on Chitosan as Edible Packaging for Cherry Tomato. J. Food Process. Preserv. 2016, 41, e13090.
    56. Mustafa, M.A.; Ali, A.; Manickam, S.; Siddiqui, Y. Ultrasound-Assisted Chitosan–Surfactant Nanostructure Assemblies: Towards Maintaining Postharvest Quality of Tomatoes. Food Bioprocess. Technol. 2014, 7, 2102–2111.
    57. Benhabiles, M.S.; Tazdait, D.; Abdi, N.; Lounici, H.; Drouiche, N.; Goosen, M.F.A.; Mameri, N. Assessment of coating tomato fruit with shrimp shell chitosan and N,O-carboxymethyl chitosan on postharvest preservation. J. Food Meas. Charact. 2013, 7, 66–74.
    58. Nascimento, J.I.G.; Stamford, T.C.M.; Melo, N.F.C.B.; Nunes, I.D.S.; Lima, M.A.B.; Pintado, M.M.E.; Stamford-Arnaud, T.M.; Stamford, N.P.; Stamford, T.L.M. Chitosan–citric acid edible coating to control Colletotrichum gloeosporioides and maintain quality parameters of fresh-cut guava. Int. J. Biol. Macromol. 2020, 163, 1127–1135.
    59. De Aquino, A.B.; Blank, A.F.; de Aquino Santana, L.C.L. Impact of edible chitosan–cassava starch coatings enriched with Lippia gracilis Schauer genotype mixtures on the shelf life of guavas (Psidium guajava L.) during storage at room temperature. Food Chem. 2015, 171, 108–116.
    60. Silva, W.B.; Silva, G.M.C.; Santana, D.B.; Salvador, A.R.; Medeiros, D.B.; Belghith, I.; da Silva, N.M.; Cordeiro, M.H.M.; Misobutsi, G.P. Chitosan delays ripening and ROS production in guava (Psidium guajava L.) fruit. Food Chem. 2018, 242, 232–238.
    61. Hu, D.; Wang, H.; Wang, L. Physical properties and antibacterial activity of quaternized chitosan/carboxymethyl cellulose blend films. LWT 2016, 65, 398–405.
    62. Awad, M.A.; Al-Qurashi, A.D.; Mohamed, S.A.; El-Shishtawy, R.M. Quality and biochemical changes of ‘Hindi-Besennara’ mangoes during shelf life as affected by chitosan, gallic acid and chitosan gallate. J. Food Sci. Technol. 2017, 54, 4139–4148.
    63. Shao, X.; Tu, K.; Tu, S.; Tu, J. A Combination of Heat Treatment and Chitosan Coating Delays Ripening and Reduces Decay in “Gala” Apple Fruit. J. Food Qual. 2012, 35, 83–92.
    64. Assis, O.B.G.; De Britto, D. Evaluation of the antifungal properties of chitosan coating on cut apples using a non-invasive image analysis technique. Polym. Int. 2011, 60, 932–936.
    65. Khalifa, I.; Barakat, H.; El-Mansy, H.A.; Soliman, S.A. Preserving apple (Malus domestica var. Anna) fruit bioactive substances using olive wastes extract-chitosan film coating. Inf. Process. Agric. 2017, 4, 90–99.
    66. Gardesh, A.S.K.; Badii, F.; Hashemi, M.; Ardakani, A.Y.; Maftoonazad, N.; Gorji, A.M. Effect of nanochitosan based coating on climacteric behavior and postharvest shelf-life extension of apple cv. Golab Kohanz. LWT 2016, 70, 33–40.
    67. Karagöz, Ş.; Demirdöven, A. Effect of chitosan coatings with and without Stevia rebaudiana and modified atmosphere packaging on quality of cold stored fresh-cut apples. LWT 2019, 108, 332–337.
    68. Pilon, L.; Spricigo, P.C.; Miranda, M.; De Moura, M.R.; Assis, O.B.G.; Mattoso, L.H.C.; Ferreira, M.D. Chitosan nanoparticle coatings reduce microbial growth on fresh-cut apples while not affecting quality attributes. Int. J. Food Sci. Technol. 2014, 50, 440–448.
    69. Chen, C.; Peng, X.; Chen, J.; Gan, Z.; Wan, C. Mitigating effects of chitosan coating on postharvest senescence and energy depletion of harvested pummelo fruit response to granulation stress. Food Chem. 2021, 348, 129113.
    70. Sharma, S.; Prasad, R.N.; Tiwari, S.; Chaurasia, S.N.S.; Shekhar, S.; Singh, J. Effect of chitosan coating on postharvest quality and enzymatic activity of eggplant (Solanum melongena L.) cultivars. J. Food Process. Preserv. 2021, 45, e15098.
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