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Bacteriophages as Fecal Pollution Indicators
Bacteriophages are promising tools for the detection of fecal pollution in different environments, and particularly for viral pathogen risk assessment. Having similar morphological and biological characteristics, bacteriophages mimic the fate and transport of enteric viruses. For this reason, enteric bacteriophages, especially phages infecting Escherichia coli (coliphages), have been proposed as alternatives or complements to fecal indicator bacteria.
Life on our planet cannot exist without water and it is estimated that 50% of the global human population lives close to rivers, lakes, or oceans . Besides its importance in maintaining health and hygiene, water is also essential for economic and productive activities (such as agriculture, industry, tourism, transportation, etc.), recreation and leisure (swimming pools, fountains, etc.), and the preservation and restoration of natural ecosystems. Therefore, a decrease in water quality due to pollution poses a risk to human wellbeing and the natural environment.
Bacteriophages have several advantages over bacterial indicators, as they are more abundant and generally more persistent in the environment and can provide more accurate information about viral pathogens. Bacteriophages serve as useful indicators of fecal contamination, as they are eliminated in feces, and do not replicate in a natural environment unless their host is present and metabolically active . They can also indicate viral contamination, as bacteriophages infecting intestinal bacteria spread into the environment in a similar way to enteric viral pathogens and have similar fates and survival patterns . Besides the technical difficulties, it would be extremely time-consuming and prohibitively expensive, especially for those countries in most urgent need of efficient water quality control.
2. Families of Bacteriophages Used as Indicators of Fecal Pollution
Bacteriophages capable of infecting enteric bacteria are generally classified into three taxonomically diverse groups: somatic coliphages, F-specific coliphages, and bacteriophages capable of infecting Bacteroides spp. . Though less common, enterophages (bacteriophages capable of infecting Enterococcus spp.) also have valuable potential as indicator organisms due to their high concentrations in wastewater, similar survival rates to enteric viruses, and differential prevalence in human or animal gut microbiota . Nevertheless, their suitability has only been tested in tropical regions and further studies are necessary .
Somatic coliphages are a heterogeneous group of bacteriophages capable of infecting E. coli and other coliform bacteria through the cell wall after becoming attached to specific receptors on the outer membrane . Under optimal physiological conditions, lysis occurs approximately 30 min after attachment, and between 100 and 1000 of progeny are released per infected cell . Double-stranded (ds) DNA Microviridae phages have tailless isometric capsids of 25–30 nm; those of the Myoviridae family have capsids of up to 100 nm and a long contractile tail; of Siphoviridae phages are up to 60 nm and have long non-contractile tails; and Podoviridae phages have isometric capsids of up to 65 nm with short tails.
Due to the availability of new genetic data, phage classification by the ICTV is currently undergoing a major overhaul, with new families being described and existing ones divided. Following this reclassification, bacteriophages capable of infecting E. coli from the Caudovirales order (dsDNA viruses) now include the new families Ackermannviridae, Autographviridae,Chaseviridae,Demerecviridae, and Drexlerviridae. As the contribution of the recently described families to phage presence in the environment has still not been clearly defined, the initial four groups of somatic coliphages (Myoviridae,Siphoviridae,Podoviridae, and Microviridae)  remain valid, as they are distinguished on a morphological basis. In contrast, some of the new families, defined by genetic differences, are indistinguishable morphologically (e.g.,Ackermannviridae and Chaseviridaeare are myophages, Autographviridae are podophages, Drexlerviridae and Demerecviridae are siphophages).
Somatic coliphages are the most abundant group of indicator bacteriophages in almost all environmental samples . Their potential replication in environments outside the gut was once a concern , but has proved to be negligible for several reasons: their narrow host range; the high concentrations of host and phages required; the possible interference of accompanying microbiota and other particles with the replicative process; the low metabolic activity of hosts in environmental conditions; and responses to environmental stresses possibly minimize phage infection . It has also been proven that neither coliphages isolated from wastewater nor laboratory stock coliphages are capable of replicating in natural samples, even in tropical climates with more conducive temperatures. It can therefore be concluded that the proportion of somatic coliphages in a natural sample arising from replication in the environment will be practically null .
They are capable of infecting E. coli and other coliform bacteria through the sexual pili, encoded in the F-plasmid, which is transferable to enteric bacteria via conjugation . This group includes two phage families: the Leviviridae, ssRNA phages with isometric tailless capsids of around 25 nm, and the Inoviridae, ssDNA phages with flexible filamentous capsids of 800 nm . Based on nucleotide analysis, the Leviviridae are divided into two genera,Levivirus and Allolevirus, which include four different genotypes of F-RNA phages: those of subgroups I and II belong to Levivirusand subgroups III and IV belong to Allolevirus genera . The study of these different subgroups is particularly useful for identifying the origin of fecal contamination, since genotypes I and IV predominate in waters contaminated by animal residues whereas genotypes II and III are commonly associated with human contamination , though these associations do not always hold, and cross-reactivity has been recorded .
F-specific bacteriophages can perform more accurately as indicators in samples where they predominate, such as groundwater, clay sediments, and reclaimed waters; they are also useful for monitoring water treatments such as UV disinfection . In contrast, they have a low persistence in surface waters, especially in warmer climates, and are readily inactivated by heat or high pH Therefore, a combination of both types of coliphages may be preferable in some types of samples. Total coliphages can be determined by either summing the results of somatic and F-specific coliphage detection assays or using a host strain that determines both in only one assay .
Concentrarion of Bacteroides phages in feces or fecally contaminated samples are usually lower compared to coliphages . Infectivity seems to be limited by the amount of bacterial capsules, which hamper phage access to the receptors . Bacteroides spp. strains are commonly used for microbial source tracking (MST) as they are strongly associated with a given human or animal host and differ in their capacity to recover phages from samples fecally contaminated by different species . For example, in southern Europe, strain B. thetaiotaomicron GA17 can detect phages of human fecal origin, unlike other strains such as B. fragilis RYC2056. Other strains such as B. thetaiotaomicron CW18, B. fragilis PG76, PL122, and PZ8 have been isolated and used to detect phages as markers of bovine, porcine, and aviary fecal contamination . Despite their low concentrations in water, Bacteroides-infecting phages are more resistant to most inactivating factors and treatments than coliphages. Their replication outside the gut is even more improbable, as the host strains are strictly anaerobic and require specific nutrients, such as hemin, that are scarcely found in the environment .
Metagenomic studies using sequences from fecal samples available in databases discovered the most abundant phage in the human fecal virome, named crAssphage (cross assembly phage) . Therefore, expanding our knowledge about crAssphage replication could help to promote their use as fecal indicators with culture techniques . Nevertheless, crAssphage have also been found in several animal sources, so further research is required on possible animal-associated variants or specific genome regions more suitable for animal source discrimination . These characteristics make crAssphage a very promising alternative as indicator microorganisms of viral fecal pollution, which could be used in MST for monitoring human fecal pollution of water .
3. Methods to Detect Bacteriophages
Strategies for detecting phages in samples can be culture-dependent or molecular, each with its own advantages, disadvantages, and appropriate applications.
Culture-dependent methods, available since phages were first discovered, provide qualitative or quantitative information about infectious phages in samples . The ISO provides standardized methodologies for detecting somatic , F-specific , andBacteroides-infecting phages  (ISO 10705), each of which includes two different approaches: a spot test (a qualitative presence/absence protocol that can be adapted to quantitative results using a most probable number approximation) and a double agar layer (DAL) assay (a quantitative protocol for counting plaque-forming units (PFU) in samples). ISO methods can be easily implemented in routine microbiology laboratories without previous experience in working with phages , and provide optional steps for laboratories with limited equipment, and quality control assays. U.S. EPA standardized protocols for detecting somatic and F-specific coliphages  also include two different methods compatible with both types of coliphages: Method 1601 (a quantitative method based on single agar layer (SAL) assays for PFU enumeration) and Method 1602 (a qualitative method based on presence/absence assays), both of which have been successfully validated, have simplified versions , and have been recently revised in Methods 1642 and 1643 .
ISO and U.S. EPA employ different host strains for the targeted phages, but their equivalent protocols usually give similar counts . To detect F-specific coliphages, host strains must express the sexual pili, encoded in the F plasmid or F-derived plasmids; those in current use areSalmonella entericaWG49 (NCTC 12484, CECT 4625, ATCC 700730)  Both strains have markers for improving strain selection and stability: Ampicilin resistance (E. coliHS/Famp) and lactose degradation capacity (S. entericaWG49)
Host strains initially proposed for the F-specific protocols could also detect somatic coliphages and were suggested for the monitoring of total coliphages, although a standardized culture method has not been described. E. coli C3000 (ATCC 15597), which is mainly used in the U.S., detects lower amounts of somatic coliphages than the standardized strains, and E. coli CB390 (CECT9198), which can recover both groups of coliphages with similar efficiency to its standardized counterparts . The standardized method for detecting Bacteroides-infecting bacteriophages uses B. fragilis RYC2056 (ATCC 700786) as a host strain, although other strains can be employed to discriminate between human and animal fecal pollution .
Standardized culture methods are simple, robust, cost-effective, and easily prepared, especially for coliphages, which do not require anaerobic growth conditions. The methods can be scaled to different sample volumes, maintaining the same proportions between medium, host strain, and sample. The material, media, reagents and labor have a similar cost to the methods currently used in routine analysis laboratories to detect fecal coliforms/E. coli. The standard methods could be optimized further to improve phage recovery and the cost/benefit relationship; modifications could include diluting the medium concentration, substituting components, and optimizing incubation protocols .
In general, standardized methods are time-consuming, requiring more than one working day to obtain reliable results (at least 18 h for coliphage plaques and more than 48 h for Bacteroides bacteriophages). To prevent potentially virally contaminated water being used for consumption, irrigation, or recreation , the results need to be obtained on the same day as the analysis. Shorter operative times and incubation periods, as well as more user-friendly handling, are also warranted by the increasing implementation of coliphages in guidelines and regulations. Several modified standardized methods have already been developed in this respect , the most promising being Easyphage and Quantiphage, which incorporate non-agar-based supports for plaque counting and previously prepared components for greater speed and simplicity .
Molecular methods, although fast and sensitive, have a major drawback in that they cannot provide information about infectivity, which therefore requires additional steps. Without infectivity data, viral concentration, and human health risks are often overestimated . Molecular methods can be serological or involve nucleic acid-based or microelectronic sensors.
Serological techniques are rapid and can be applied in situ, but they require pre-enrichments, and antisera are less available than nucleic acid probes and primers . They are mainly applied to detect F-specific coliphages, using latex agglutination or neutralization methods , being less suitable for the highly diverse and complex somatic coliphages, though CLAT- (Culture, Latex Agglutination, and Typing) based analyses have been developed for some specific families .
Nucleic acid methods are based on plaque hybridization, employing specific probes or, more frequently, qPCR/RTqPCR assays. In plaque hybridization methods, specific probes designed for each targeted phage or phage group are applied to plaques obtained by culture . The quantities detected by molecular methods tend to be higher compared with culture methods, as GC signals are more persistent in the environment and more resistant to treatments than infectious viruses . PCR-based approaches can also be inhibited by organic substances such as phenolic compounds, which are occasionally present in environmental samples .
Microelectronic methods involve the detection of viral particles, or the lysis of host bacteria caused by bacteriophage infection . Although fast, with results being obtained in less than 1 h, their sensitivity and precision do not yet match DAL-based methods . Their usage is normally restricted to the detection of a specific phage, rather than to analyze environmental samples containing different phages at varying concentrations. Consequently, no microelectronic method so far has achieved a useful or feasible application to determine infectious bacteriophages as indicators of fecal pollution in environmental samples.
Viral concentration methods to optimize detection processes have been developed in parallel with standardized methodologies. For the analysis of larger volumes, the samples need to be concentrated, especially if quantification is required, as in drinking water samples with low levels of contamination. When turbidity is low, a simple, inexpensive, and practical procedure is recommended, using mixed cellulose and acetate membrane filters with a pore size of 0.45 μm after the addition of salts and pH adjustment . When turbidity is high, flocculation with magnesium hydroxide is feasible for all three groups of fecal indicator phages .
4. Application of Bacteriophages as Indicators of Fecal Pollution
As already mentioned, FIB are used to estimate the microbiological quality of water, but they may not be suitable or sufficiently reliable to predict the presence of enteric viruses. In general, enteric viruses have higher survival rates during wastewater and drinking water treatment than bacterial indicators and greater persistence in environmental waters . Therefore, the use of bacterial indicators alone could underestimate the microbiological contamination of water and the associated human health risks. Adding at least one viral indicator to the analysis provides a more accurate assessment of water quality and promotes more confidence in its safety.
The primary origin of coliphages in water environments are human and animal feces. They can reach water through raw or treated human and animal wastewater, septic tank overflow, sewer leakage, and the spread of solid waste (sewage sludge, slurry, manure, and the feces of pets, farmed animals, and wild animals) . As early as 1948, Guelin  already saw the potential of coliphages as indicators of enteric microorganisms in water, observing their good correlation with the numbers of coliform bacteria in fresh and marine water. Since then, many other studies have assessed the potential of bacteriophages as indicators of fecal contamination in different water environments (Figure 1):
Wastewater treatment plants: Bacteriophages are considered to be useful tools to evaluate the efficacy of wastewater treatment plants  because their reduction by certain pathogen removal methods is similar to that of human enteric viruses  whereas the reduction of traditional FIB is significantly higher . Coliphage concentration in wastewater shows no seasonality and remains consistently high throughout the year worldwide, as occurs with bacterial indicators . Coliphage densities in wastewater are quite variable but the lower density of F-specific coliphages compared to somatic coliphages in both treated and untreated wastewater sources , potentially limits their use as indicators in this environment.
Drinking water : The presence of coliphages or Bacteroides-infecting phages in drinking water sources is a likely indicator of fecal contamination or an inadequate treatment . Generally, the levels of bacterial indicators, viruses, and bacteriophages in these sources are low and seldom detected after treatment. The few reports describing the potential of phages to assess the quality of drinking water suggest once again that they outperform conventional FIB, undergoing less reduction after different drinking water treatments .
Recreational water: The sanitary quality of recreational waters is monitored using FIB according to the EU Directive 2006/7/EC , but alternative indicators, such as Clostridium perfringens and bacteriophages, have also been proposed . Somatic coliphages are found in recreational water , and at beaches with unknown sources of fecal contamination, the presence of coliphages correlates with the occurrence of diseases more often than the presence of FIB . It has been reported that in waters with detectable coliphages there was an increased incidence of gastrointestinal illness among bathers when fecal pollution was likely present, but not otherwise . These findings indicate that coliphages may be suitable for application as indicators of bathing water quality.
Groundwater: Contaminants reaching surface waters also affect groundwater, the routes including failure in septic systems, leaking sewer lines and passage through soils and fissures. A study shows that one bacterial indicator and one phage indicator provide more information than two bacterial indicators when assessing the microbiological quality of groundwater . However, to date, only one regulation (2006) includes bacteriophages as indicators of enteric viral pollution in groundwater, reflecting that further research is needed in this area.
Solid or semisolid matrices play an important role in the persistence and dispersion of pathogens in the water cycle, as they can contain large amounts of pathogens, especially viruses, if contaminated with fecal waste .
To optimize the use of bacteriophages as indicators in solid matrices (Figure 1), standardized methodologies for their extraction, detection, and enumeration need to be developed. Nevertheless, in general, studies indicate that somatic coliphages are found in solid matrices at higher levels than traditional FIB and F-specific RNA coliphages. In addition, they persist longer in soils and sediments and are more resistant to sludge and manure treatments. For an extensive review of this question, and more data on solid matrices, see Martín-Díaz et al.
Coliphages can be found in food when fecally contaminated water is used to grow vegetables and fruits, in meat processing, or to farm shellfish. Bivalve shellfish are regularly implicated in foodborne viral disease outbreaks because there is no effective way to rid them of viral contamination without changing their sensory characteristics. However, the utility of phages as routine indicators of viral pollution in shellfish, in contaminated sites, or under normal growing conditions, is controversial; studies with conflicting results have been reviewed . Regardless, the use of bacterial indicators alone is clearly insufficient to prevent viral disease outbreaks stemming from shellfish consumption  and more accurate data about enteric viruses could be provided by the addition of a viral indicator.
Regarding fish, a comparative study found somatic coliphages in Atlantic, farmed and frozen fish, with 30%, 10%, and 20% of the samples testing positive, respectively; in contrast E. coli was only found in 10% of the Atlantic fish samples .
Hsu et al. found somatic coliphages in 88% of ground meat and poultry meat samples and F-specific coliphages in 63%. They also evaluated the risk of fecal contamination at three control points (evisceration, washing and chilling) and observed that the reduction of F-specific coliphages during these processing steps matched that of FIB . Somatic coliphages have also been reported in minced pork, minced chicken, and ham, with 60%, 100%, and 40% of the samples testing positive, respectively. No E. coli was found in ham samples, and only 30% of minced pork and 90% of minced chicken samples tested positive for this bacterial indicator .
In a study of animal feeds, Maciorowski et al. analyzed animal feeds, feed ingredients, and poultry diets for the presence of coliphages, finding somatic and F-specific coliphages in all the tested samples, even after 14 months of storage at −20 °C .
Bacteriophages can also be used as fecal indicators in vegetables, as they have been found in lettuce and cucumber . In lettuce, the number of samples positive forE. coliwas slightly higher compared to somatic coliphages (50% and 40%, respectively); however, 20% of cucumber samples were positive for coliphages and none containedE. coli.
All these data suggest bacteriophages perform well as fecal indicators in food (Figure 1) as they seem to remain longer in the different food matrices than bacteria.
The entry is from 10.3390/v13061089
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