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Nielsen, B.; Milledge, J. Antioxidants. Encyclopedia. Available online: https://encyclopedia.pub/entry/9447 (accessed on 19 April 2024).
Nielsen B, Milledge J. Antioxidants. Encyclopedia. Available at: https://encyclopedia.pub/entry/9447. Accessed April 19, 2024.
Nielsen, Birthe, John Milledge. "Antioxidants" Encyclopedia, https://encyclopedia.pub/entry/9447 (accessed April 19, 2024).
Nielsen, B., & Milledge, J. (2021, May 10). Antioxidants. In Encyclopedia. https://encyclopedia.pub/entry/9447
Nielsen, Birthe and John Milledge. "Antioxidants." Encyclopedia. Web. 10 May, 2021.
Antioxidants
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This entry is adapted from the peer-reviewed paper 10.3390/app11093959

There are established links between free radicals and more than sixty different human health conditions, including ageing, cancer, diabetes, Alzheimer’s disease, strokes, heart attacks, and atherosclerosis. Consumption of higher levels of dietary antioxidant enzymes and antioxidant molecules-enriched food or antioxidant supplements has been found to reduce the risk of free radical-related health issues. The green halotolerant microalgae Dunaliella can grow in a wide range of saline environments and is a  potential natural source for antioxidant production.

antioxidant enzymes cultivation conditions

1. Classification of Antioxidants

Antioxidants (enzymatic or non-enzymatic) are classified depending on their mode of action as primary antioxidants (hydrogen or electrons donors) or secondary antioxidants (oxygen scavengers or chelating agents) [1][2]. Antioxidants can also be grouped according to size, solubility, or structure, Figure 1. Abundant enzymatic antioxidants in microalgae are superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione transferase (GST). Non-enzymatic antioxidants consist of compounds such as Vitamin C (ascorbate), glutathione, carotenoid, phenolic compounds, proline, glycine, polyamine, PUFA, and some metals (Cu, Zu) [3][4][5]. Most enzymatic antioxidants and some non-enzymatic antioxidants (glutathione, ascorbate) are hydrophilic and mainly present in the cellular fluids (cytosol or cytoplasmic matrix), whereas the hydrophobic antioxidants (carotenoid, tocopherol) are primary located in the cell membranes [6].
Applsci 11 03959 g001 550
Figure 1. Classification of antioxidants [7][2][6][8][9].

2. Antioxidant Enzymes

Antioxidant enzymes prevent or delay the oxidation of other molecules by neutralising reactive oxygen species (ROS) [6]. These enzymes eliminate ROS by reducing the energy of free radicals or by donating electrons to free radicals [10], and as such, constitute the first level of defence in the cell's antioxidant network. Some molecules are not involved directly in the scavenging of free radicals but rather enhance other antioxidant molecules’ activity and may also be classified as antioxidants [7]. The majority of antioxidant enzymes are metalloenzymes and contain a metal ion in their catalytic site [3].

3. Commercial Applications of Antioxidant Enzymes

Antioxidants, or antioxidant-enriched extracts, are commercially used to prevent oxidative processes and to maintain the flavor, texture, and colour of food during storage. They also find uses as refining, bleaching, deodorising agents in the food processing industries [11][12][13][14][15], in extending the shelf life of lubricating oil and reducing vehicular emissions [16] and in stabilisation of synthetic fibre, rubber, thermoplastic, and adhesives by stopping autocatalytic reactions [17]. In cosmeceutical products, antioxidant compounds are used to prevent skin ageing and UV-induced skin damage and treat the appearance of wrinkles and erythema [18][19][20][21]. Industrial applications of antioxidants are listed in Table 1. The global demand for antioxidants was valued at ~USD 2.25 billion in 2014 and grew at a CAGR (compounds annual growth rate) of ~5.5% between 2015 and 2020 [22]. This increasing global demand is driving the search for synthetic and natural-derived antioxidants.
Table 1. Commercial uses of enzymatic and non-enzymatic antioxidant.
Antioxidants Applications Ref
Natural antioxidants SOD Added to cosmetic products to protect against skin damage [8][12][20][23][24]
Protect against lipid peroxidation, heat, and cold stress in poultry production
As a therapeutic agent for treatment of inflammatory disorders
Normal cells protector during radiotherapy for cancer patients
CAT Eliminate excessive H2O2 in the textile industry, pulp, and paper industry used for bleaching fibres and pulp, and as a bactericidal disinfectant in food processing and the pharmaceutical industry [11][17][25]
In aesthetics (mask treatment) to increase cellular oxygenation in the upper layers of facial epidermis
Reducing the risk of diabetes mellitus
GPX Immune system booster [10][26]
GST Protective role against neurogenerative diseases [26][27]
Decreases the risk of tumours of the head and neck, oral, cavity and colon
Glutathione Anti-wrinkle formation, and as a modifier of skin smoothness [28]
Vitamins As a food preservative and bread improver, protective activity against heart diseases, reduced the risk of colorectal adenomas and prostate cancer, reduction of thyroid hormone levels [29][30][31][32][33]
Flavonoids As cancer preventive agents, protection against type 2 diabetes Functional food additive [34][35][36][37]
Carotenoid Anticancer agents, additive to cosmetics and multivitamin preparation
Food colouring agent, pro-vitamin A in food and animal feed		 [38][39][40]
PUFA Prevention of heart and inflammatory diseases [41][42]
Synthetic Antioxidants BHA Extending the shelf life of vegetable oil, frying oil, animal feed, cereals, chewing gum, potato flakes and cosmetic products [43][44][45]
BHT Increasing the shelf life of animal fats, chewing gum, animal feed, vegetable oils [44][45]
TBHQ Used as preservative for enhancing storage stability of vegetable oils, margarine, fish oil, fried foods, essential oils, nuts, edible animal fats, butterfat, and packed fried foods [44]
Propyl gallate As an antioxidant agent in foods and vegetable oil [44]
BHA = butylated hydroxyl anisole; TBHQ = tert-butylhydroquinone; BHT = butylated hydroxyl toluene
Commercially, synthetic antioxidants such as butylated hydroxyl anisole (BHA), butylated hydroxyl toluene (BHT), α-tocopherol and propyl gallate are used in foods, food packaging, cosmetics and pharmaceutical products [44][46]. However, the physical properties of BHT and BHA (high volatility and instability at elevated temperature), strict legislation on the use of synthetic food additives, and the carcinogenic nature of some synthetic antioxidants [47][48][49][50] have shifted the attention to finding antioxidants from natural sources that are pharmacologically potent and have low or no toxicity.

4. Generation and Detoxification of ROS

ROS is a collective term for oxygen-derived products (free radicals and non-radicals reactive derivatives of oxygen). They are produced in cellular compartments either exogenously (in response to environmental stress such as UV radiation or xenobiotics) or endogenously (from the intracellular metabolic pathway, enzymatic activities, mitochondrial respiration, or photosynthesis) [26]. Accumulation of ROS leads to oxidative stress in cells and causes damage to cellular macromolecules, including proteins, lipids, carbohydrates and DNA [51]. Various sources of ROS and corresponding modes of biochemical metabolism are summarised in Table 2.
Table 2. Production of ROS in cells during biological metabolism and their corresponding neutralising antioxidants.
ROS Reaction Life Span Function Sources of ROS Scavenging Antioxidants
Singlet (1O2)
First excited electronic state of O2		 Chlorophyll triplet state (Chl) is generated sue to insufficient energy dissipation during photosynthesis
Chl + 3O2 → 1O2
Reduction of transition metal (Fe3+)
O2•− + Fe3+ → 1O2 + Fe2+		 3 µs (appx.)
4 µs in H2O
100 µs in polar solvents		 Gene up-regulation, molecular defense against photo-oxidative stress Chloroplast β-carotene, lycopene, tocopherol, ASc, plastoquinone, and proline
Superoxide radical
(O2•−)
One electron reduction of 3O2		 Reduction of oxygen (3O2) during electron transport during the photosynthesis process in chloroplasts or during oxidative phosphorylation in the mitochondria
3O2 + e− → O2•−
3O2 + Xanthine (Xanthine oxidase) → O2•− + uric acid
3O2 + NADPH (NADPH oxidase) → O2•− + NADP+ + H+		 2–4 µs Triggering the formation of more ROS which further participates in membrane lipid peroxidation PSI in Chloroplast
oxidative phosphorylation in mitochondria
Peroxisomes
Plasma membrane		 SOD, ASc, glutathione (GSH), flavonoids, Cu
H2O2
Two electron reduction of 3O2 or univalent reduction of O2•−		 Protonation reaction (acidic conditions)
O2•− + H+ + HO2• →H2O2 + 2O2
Glycolate + O2 (Glycolate oxidase) → H2O2 + Glyoxylate
Fatty acids (β−oxidation) → Acetyl coenzyme + H2O2		 1 ms Act as a signaling molecule (low concentration of H2O2), at high concentration of H2O2 triggers tolerance to various stress, as a regulator of physiological processes (photorespiration and photosynthesis) Chloroplast, Mitochondria, and
Peroxisome		 CAT, POD (GPX and APX), peroxiredoxin, ASc, tocopherol, GSH, β-carotene, Se flavonoids, lipoic acid,
HO2• Protonation of superoxide ions
O2•− + H+ → HO2•		   Attacks PUFA in the negatively charged membrane surface Mitochondria, microsomes and peroxisomes  
HO• (Three electron reduction of 3O2) Fenton reaction:
H2O2 + Fe2+ → HO• + HO− + Fe3+
Haber-Weiss reactions:
O2•− + H2O2 → HO• + HO− + O2		 1 ps Attack unsaturated fatty acids in membranes Mitochondria ASc, GSH, flavonoids, lipoic acid, proline
Nitric oxide (NO•) L-arginine + O2 Nitric oxide synthase → NO• + Citrulline   Intercellular messenger, the quencher of O2•−, defense against various protozoa, fungi, and mycobacteria Peroxisome, cytosol GSH
Peroxynitrite (ONOO−) NO• + O2•− → ONOO−   React with amino acids residues in enzymes causing inactivation   Peroxiredoxin, Uric acid
Lipid hydroperoxide Oxidation of PUFA   Tissue injuries and diseases Mitochondrial membrane PUFA  
The majority of ROS are generated when electrons leak from the chloroplastic electron transport systems during photosynthesis, from the mitochondrial electron transport chain during photorespiration, and from the peroxisomal membrane electron transport chain (Figure 2) [52]. Within plant cells, 1–2% of O2 consumption leads to the formation of superoxide (O2•−), and 1–5% of mitochondrial O2 consumption leads to the generation of H2O2 [3]. O2•− is generated during oxidisation of unsaturated fatty acids [53], from the activity of cytochrome P450 [54] and the cytochrome b5 family members [55][56]. Also, O2•− is produced in the peroxisome where xanthine oxidases catalyse the oxidation of xanthine and hypoxanthine to uric acid [3], and in the plasma membrane due to the reduction of O2 by NADPH oxidases [8].
Applsci 11 03959 g002 550
Figure 2. Generation of ROS by energy transfer and during intracellular metabolic pathways in microalgae (FD; ferredoxin, FNR; ferredoxin-NADP+ oxidoreductase; PSI-Photosystem I; PSII-Photosystem II, PC-plastocyanin [52][57]).
Superoxide ions (O2•−) are converted into hydrogen peroxide (H2O2) by the catalytic activity of SOD. H2O2 is also produced in peroxisomes when glycolate from the photorespiration is recycled. In addition, H2O2 can be formed by D-amino acid oxidase, urate oxidase, flavin oxidase, L-α-hydroxy acid oxidase, and fatty acyl-CoA oxidase, and by cell wall peroxidases. Reactive OH• is produced from the reaction of O2•− and H2O2 at neutral pH and ambient temperature (Haber–Weiss reaction) or from H2O2 during the Fenton reaction [58]. In contrast, a proton (H+) addition to O2•− generates perhyroxyl radicals (HO2•). In some cases, intracellular ROS could also form during auto-oxidation of small molecules (epinephrine, flavins, and hydroquinones) [59][60]. The singlet oxygen (1O2) is generated from the reaction of oxygen (3O2) with the triplet state of chlorophyll produced by the dissipation of insufficient energy during photosynthesis [3].
Under normal physiological conditions, ROS is neutralised by the cells’ antioxidant systems where antioxidant enzymes and antioxidant molecules maintain the delicate intracellular redox balance and mitigate undesirable cellular damage caused by ROS, Table 2 [6][9].
Different isozymes of SOD exist (Mn-SOD in mitochondria and peroxisome, Fe-SOD in the chloroplast, and Cu/Zn-SOD isozyme in cytosol) but they all participate in scavenging of O2•−. In addition to SOD, some antioxidants molecules (Vitamin C, glutathione, etc.) also eliminate O2•− [3]. Further, the individual or cumulative catalytic activity of catalase or peroxidases decomposes H2O2 into H2O and O2. CAT, peroxidases (GPX, APX), and SOD show a synergistic effect in the scavenging of O2•−. In addition to eliminating H2O2, GPX can protect cells by preventing intracellular lipid peroxidation [6]. APX may be more efficient compared to CAT or GPX in detoxification of H2O2 due to its higher affinity for H2O2. APX reduces H2O2 into H2O in chloroplasts, cytosol, mitochondria, and peroxisomes, and in the apoplastic space using Ascorbic acid (ASc) as an electron donor [61].
Algal cells accumulate ASc with 30–40% remaining in the chloroplast. Ascorbic acid is water-soluble and acts as a potent antioxidant because of its ability to donate electrons in enzymatic and non-enzymatic reactions [62]. It protects cells by directly scavenging O2•−, HO2•− and regenerating the tocopherol from tocopheroxyl radicals [63]. All intracellular compartments generate the reduced form of glutathione, which plays a role as an excellent scavenger of many ROS such as O2•−, HO•, O3, NO2, lipid hydroperoxides [9] due to the redox-active thiol group that becomes oxidised when GSH reduces ROS [64]. Carotenoid also protects cells from light-induced oxidative stress by quenching 1O2 or dissipating excess heat (excitation energy) or scavenging peroxy radicals [3][6].
As the accumulation of enzymatic and non-enzymatic antioxidants in the cell depends on the external environment, manipulating cultivation conditions could enhance the intracellular antioxidant levels.

References

  1. Rahal, A.; Kumar, A.; Singh, V.; Yadav, B.; Tiwari, R.; Chakraborty, S.; Dhama, K. Oxidative stress, prooxidants, and antioxidants: The interplay. Biomed. Res. Int. 2014, 2014, 761264.
  2. Panda, S.K. Assay Guided Comparison for Enzymatic and Non-Enzymatic Antioxidant Activities with Special Reference to Medicinal Plants. In Antioxidant Enzyme; Intech: Rijeka, Croatia, 2012; Volume 14, pp. 381–400. Available online: (accessed on 26 April 2021).
  3. Gill, S.S.; Tuteja, N. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 2010, 48, 909–930.
  4. Mtaki, K.; Kyewalyanga, M.S.; Mtolera, M.S.P. Assessment of antioxidant contents and free radical-scavenging capacity of chlorella vulgaris cultivated in low cost media. Appl. Sci. 2020, 10, 8611.
  5. Rezayian, M.; Niknam, V.; Ebrahimzadeh, H. Oxidative damage and antioxidative system in algae. Toxicol. Rep. 2019, 6, 1309–1313.
  6. Nimse, S.B.; Pal, D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Adv. 2015, 5, 27986–28006.
  7. Rahman, K. Studies on free radicals, antioxidants, and co-factors. Clin. Interv. Aging 2007, 2, 219–236.
  8. Kaushal, J.; Mehandia, S.; Singh, G.; Raina, A.; Arya, S.K. Catalase enzyme: Application in bioremediation and food industry. Biocatal. Agric. Biotechnol. 2018, 16, 192–199.
  9. Gopal, R.K.; Elumalai, S. Industrial Production of Superoxide Dismutase (SOD): A Mini Review. J. Probiotics Health 2017, 5.
  10. Krishnamurthy, P.; Wadhwani, A. Antioxidant Enzymes and Human Health. In Antioxidant Enzyme; Intech: Rijeka, Croatia, 2012; Volume 1, pp. 3–18. Available online: (accessed on 26 April 2021).
  11. Pandey, V.P.; Awasthi, M.; Singh, S.; Tiwari, S.; Dwivedi, U.N. A Comprehensive Review on Function and Application of Plant Peroxidases. Biochem. Anal. Biochem. 2017, 6, 1–16.
  12. Keokamnerd, T.; Acton, J.C.; Han, I.Y.; Dawson, P.L. Effect of commercial rosemary oleoresin preparations on ground chicken thigh meat quality packaged in a high-oxygen atmosphere. Poult. Sci. 2008, 87, 170–179.
  13. Hamid, M. Khalil-ur-Rehman Potential applications of peroxidases. Food Chem. 2009, 115, 1177–1186.
  14. Canter, N. Use of antioxidants in automotive lubricants. Tribol. Lubr. Technol. 2008, 64, 12.
  15. Jegannathan, K.R.; Nielsen, P.H. Environmental assessment of enzyme use in industrial production-a literature review. J. Clean. Prod. 2013, 42, 228–240.
  16. Godic, A.; Poljšak, B.; Adamic, M.; Dahmane, R. The role of antioxidants in skin cancer prevention and treatment. Oxid. Med. Cell. Longev. 2014, 2014, 860479.
  17. Allemann, I.B.; Baumann, L. Antioxidants used in skin care formulations. Ski. Ther. Lett 2008, 13, 5–9.
  18. Stephenie, S.; Chang, Y.P.; Gnanasekaran, A.; Esa, N.M.; Gnanaraj, C. An insight on superoxide dismutase (SOD) from plants for mammalian health enhancement. J. Funct. Foods 2020, 68, 103917.
  19. Bafana, A.; Dutt, S.; Kumar, S.; Ahuja, P.S. Superoxide dismutase: An industrial perspective. Crit. Rev. Biotechnol. 2011, 31, 65–76.
  20. Beach, D. Antioxidants (Natural, and Synthetic) Market for Pharmaceuticals, Food & Beverages Sector, Feed Additives, Cosmetics Industry, and Other Applications: Global Industry Perspective, Comprehensive Analysis, and Forecast, 2014–2020, Marrket Research Store. 2016. Available online: (accessed on 26 April 2021).
  21. Sasse, A.; Colindres, P.; Brewer, M.S. Effect of natural and synthetic antioxidants on the oxidative stability of cooked, frozen pork patties. J. Food Sci. 2009, 74, 1–6.
  22. Schillaci, C.; Nepravishta, R.; Bellomaria, A. Antioxidants in food and pharmaceutical research. Albanian J. Pharm. Sci. 2014, 1, 15–25.
  23. Ito, N.; Hirose, M.; Fukushima, S.; Tsuda, H.; Shirai, T.; Tatematsu, M. Studies on antioxidants: Their carcinogenic and modifying effects on chemical carcinogenesis. Food Chem. Toxicol. 1986, 24, 1071–1082.
  24. Thompson, D.C.; Trush, M.A. Studies on the mechanism of enhancement of butylated hydroxytoluene-induced mouse lung toxicity by butylated hydroxyanisole. Toxicol. Appl. Pharmacol. 1988, 96, 122–131.
  25. Silaghi-dumitrescu, L.; Loghin, F. Evaluation of the possible endocrine disruptive effect of butylated hydroxyanisole, butylated hydroxytoluene and propyl gallate in immature female rats. Farmacia 2013, 61, 202–211.
  26. Thorat, I.D.; Jagtap, D.D.; Mohapatra, D.; Joshi, D.C.; Sutar, R.F.; Kapdi, S.S. Antioxidants, their properties, uses in food products and their legal implications. Int. J. Food Stud. 2013, 2, 81–104.
  27. El Shafey, H.M.; Bahashwan, S.A.; Alghaithy, A.A.; Ghanem, S. Microbial superoxide dismutase enzyme as therapeutic agent and future gene therapy. Curr. Res. Technol. Educ. Top. Appl. Microbiol. Microb. Biotechnol. 2010, 1, 435–443.
  28. Surai, P.; Surai, P.F. Antioxidant Systems in Poultry Biology: Superoxide Dismutase. Anim. Nutr. 2015, 1, 1–17.
  29. Gridley, D.S.; Green, L.M.; Nelson, G.A.; Pecaut, M.J.; Slater, J.M. Therapeutic Utilities of SOD Mimetics: Cancer, Radiotherapy and SOD Mimetics. In Madame Curie Bioscience Database [Internet]; Landes Bioscience: Austin, TX, USA, 2013. Available online: (accessed on 26 April 2021).
  30. Miłek, J.; Wójcik, M.; Verschelde, W. Thermal stability for the effective use of commercial catalase. Polish J. Chem. Technol. 2014, 16, 75–79.
  31. Morón, Ú.M.; Castilla-Cortázar, I. Protection Against Oxidative Stress and “IGF-I Deficiency Conditions”. In Antioxidant Enzyme; Intechopen: Rijeka, Croatia, 2012; Available online: (accessed on 26 April 2021).
  32. Chenevix Trench, G.; Young, J.; Coggan, M.; Board, P. Glutathione S-transferase M1 and T1 polymorphisms: Susceptibility to colon cancer and age of onset. Carcinogenesis 1995, 16, 1655–1657.
  33. Hashizume, E.; Chan, G.P. Skin-whitening and skin-condition-improving effects of topical oxidized glutathione: A double- blind and placebo-controlled clinical trial in healthy women. Clin. Cosmet. Investig. Dermatol. 2014, 267–274.
  34. Barrita, J.; Sánchez, M. Antioxidant Role of Ascorbic Acid and His Protective Effects on Chronic Diseases. Oxidative Stress Chronic Degener. Dis. A Role Antioxid. 2013, 450–484.
  35. Borek, C. Antioxidants and the prevention of hormonally regulated cancer. J. Men’s Health Gend. 2005, 2, 346–352.
  36. Constantinou, C.; Papas, A.; Constantinou, A.I. Vitamin E and cancer: An insight into the anticancer activities of vitamin E isomers and analogs. Int. J. Cancer 2008, 123, 739–752.
  37. Petrulea, M.; Muresan, A.; Duncea, I. Oxidative Stress and Antioxidant Status in Hypo- and Hyperthyroidism. In Antioxidant Enzyme; Intechopen: Rijeka, Croatia, 2012; Available online: (accessed on 26 April 2021).
  38. Bhalamurugan, G.L.; Valerie, O.; Mark, L. Valuable bioproducts obtained from microalgal biomass and their commercial applications: A review. Environ. Eng. Res. 2018, 23, 229–241.
  39. Singh, P.; Baranwal, M.; Reddy, S.M. Antioxidant and cytotoxic activity of carotenes produced by Dunaliella salina under stress. Pharm. Biol. 2016, 54, 2269–2275.
  40. Kaliora, A.C.; Dedoussis, G.V.Z.; Schmidt, H. Dietary antioxidants in preventing atherogenesis. Atherosclerosis 2006, 187, 1–17.
  41. Sak, K. Cytotoxicity of dietary flavonoids on different human cancer types. Pharmacogn. Rev. 2014, 8, 122–146.
  42. Vinayagam, R.; Xu, B. Antidiabetic properties of dietary flavonoids: A cellular mechanism review. Nutr. Metab. 2015, 12, 1–20.
  43. Jiang, W.; Wei, H.; He, B. Dietary flavonoids intake and the risk of coronary heart disease: A dose-response meta-analysis of 15 prospective studies. Thromb. Res. 2015, 135, 459–463.
  44. Hertog, M.G.L.; Feskens, E.J.M.; Kromhout, D.; Hertog, M.G.L.; Hollman, P.C.H.; Hertog, M.G.L.; Katan, M.B. Dietary antioxidant flavonoids and risk of coronary heart disease: The Zutphen Elderly Study. Lancet 1993, 342, 1007–1011.
  45. Yaakob, Z.; Ali, E.; Zainal, A.; Mohamad, M.; Takriff, M.S. An overview: Biomolecules from microalgae for animal feed and aquaculture. J. Biol. Res. 2014, 21, 6.
  46. Martins, D.A.; Custódio, L.; Barreira, L.; Pereira, H.; Ben-Hamadou, R.; Varela, J.; Abu-Salah, K.M. Alternative sources of n-3 long-chain polyunsaturated fatty acids in marine microalgae. Mar. Drugs 2013, 11, 2259–2281.
  47. Harris, W.S.; Mozaffarian, D.; Rimm, E.; Kris-Etherton, P.; Rudel, L.L.; Appel, L.J.; Engler, M.M.; Engler, M.B.; Sacks, F. Omega-6 fatty acids and risk for cardiovascular disease: A science advisory from the American Heart Association nutrition subcommittee of the council on nutrition, physical activity, and metabolism; council on cardiovascular nursing; and council on epidem. Circulation 2009, 119, 902–907.
  48. Lundebye, A.-K.; Hove, H.; Måge, A.; Bohne, V.J.B.; Hamre, K. Levels of synthetic antioxidants (ethoxyquin, butylated hydroxytoluene and butylated hydroxyanisole) in fish feed and commercially farmed fish. Food Addit. Contam. Part A Chem. Anal. Control. Expo. Risk Assess. 2010, 27, 1652–1657.
  49. Dawidowicz, A.L.; Olszowy, M.; Jóźwik-Dolęba, M. Antagonistic Antioxidant Effect in Butylated Hydroxytoluene/Butylated Hydroxyanisole Mixture. J. Food Process. Preserv. 2015, 39, 2240–2248.
  50. Mittler, R.; Vanderauwera, S.; Gollery, M.; Van Breusegem, F. Reactive oxygen gene network of plants. Trends Plant Sci. 2004, 9, 490–498.
  51. Cirulis, J.T.; Scott, J.A.; Ross, G.M. Management of oxidative stress by microalgae. Can. J. Physiol. Pharmacol. 2013, 91, 15–21.
  52. Kaushik, D.; Roychoudhury, A. Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants. Front. Environ. Sci. 2014, 2, 53.
  53. Bak, S.; Beisson, F.; Bishop, G.; Hamberger, B.; Höfer, R.; Paquette, S.; Werck-Reichhart, D. Cytochromes P450. Arab. B 2011, 9, e0144.
  54. Martin-Montalvo, A.; Sun, Y.; Diaz-Ruiz, A.; Ali, A.; Gutierrez, V.; Palacios, H.H.; Curtis, J.; Siendones, E.; Ariza, J.; Abulwerdi, G.A. Cytochrome b5 reductase and the control of lipid metabolism and healthspan. NPJ Aging Mech. Dis. 2016, 2, 16006.
  55. Pruchniak, M.P.; Araźna, M.; Demkc, U. Biochemistry of oxidative stress. Adv. Exp. Med. Biol. 2016, 878, 9–19.
  56. Barbusiński, K. Controversy Over Fenton Mechanism. Ecol. Chem. Eng. S 2009, 16, 347–358.
  57. Tatsumi, H.; Nakase, H.; Kano, K.; Ikeda, T. Mechanistic study of the autoxidation of reduced flavin and quinone compounds. J. Electroanal. Chem. 1998, 443, 236–242.
  58. Vašková, J.; Vaško, L.; Kron, I. Oxidative Processes and Antioxidative Metaloenzymes. In Antioxidant Enzyme; Intechopen: Rijeka, Croatia, 2012; Volume 2, pp. 19–58. Available online: (accessed on 26 April 2021).
  59. Gupta, V.K.; Sharma, S.K. Plants as Natural Antioxidants. Indian J. Nat. Prod. Resour. 2006, 5, 326–334.
  60. Sofo, A.; Scopa, A.; Nuzzaci, M.; Vitti, A. Ascorbate peroxidase and catalase activities and their genetic regulation in plants subjected to drought and salinity stresses. Int. J. Mol. Sci. 2015, 16, 13561–13578.
  61. Sharma, P.; Jha, A.B.; Dubey, R.S.; Pessarakli, M. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. J. Bot. 2012, 2012, 217037.
  62. Mallick, N.; Mohn, F.H. Reactive oxygen species: Response of algal cells. J. Plant Physiol. 2000, 157, 183–193.
  63. Marí, M.; Morales, A.; Colell, A.; García-Ruiz, C.; Fernández-Checa, J.C. Mitochondrial glutathione, a key survival antioxidant. Antioxid. Redox Signal. 2009, 11, 2685–2700.
  64. Jajic, I.; Sarna, T.; Strzalka, K. Senescence, Stress, and Reactive Oxygen Species. Plants 2015, 4, 393–411.
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